Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              20     509
NP_001075109                                                           20     509
NP_054790                                                              16    5631
XP_006723818                                                           16    5631
XP_006723817                                                           16    5631
XP_006723816                                                           16    5631
XP_006723815                                                           16    5631
XP_006723814                                                           16    5631
XP_006723813                                                           16    5631
XP_005260405                                                           16    5631
NP_001094058                                                           16    7355
NP_061323                                                              16    7355
XP_006716433                                                           16    7355
XP_005273633                                                           16    7355
XP_006716432                                                           16    7355
XP_005273632                                                           16    7355
NP_079033                                                              15    9605
NP_001275915                                                           15    9605
NP_001275914                                                           15    9605
NP_001537                                                              15    9605
NP_009099                                                              15    9605
NP_005654                                                              15    9605
NP_056052                                                              15    9605
NP_079467                                                              15    9605
NP_001138648                                                           15    9605
XP_005256997                                                           15    9605
XP_005256995                                                           15    9605
XP_006717353                                                           15    9605
XP_006717352                                                           15    9605
XP_005266166                                                           15    9605
XP_005266167                                                           15    9605
XP_005266165                                                           15    9605
XP_005266164                                                           15    9605
XP_006717351                                                           15    9605
XP_005266163                                                           15    9605
XP_005266162                                                           15    9605
XP_006713980                                                           15    9605
XP_006713979                                                           15    9605
XP_005245556                                                           15    9605
>NP_008835
          Length = 4128

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    ANALNSEPA 9
            ANAL+SEPA
Sbjct: 3338 ANALSSEPA 3346
>NP_001075109
          Length = 4097

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    ANALNSEPA 9
            ANAL+SEPA
Sbjct: 3338 ANALSSEPA 3346
>NP_054790
          Length = 2063

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    NALNSEPA 9
            NAL+S PA
Sbjct: 1716 NALSSSPA 1723
>XP_006723818
          Length = 1975

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    NALNSEPA 9
            NAL+S PA
Sbjct: 1716 NALSSSPA 1723
>XP_006723817
          Length = 2020

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    NALNSEPA 9
            NAL+S PA
Sbjct: 1673 NALSSSPA 1680
>XP_006723816
          Length = 2034

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    NALNSEPA 9
            NAL+S PA
Sbjct: 1716 NALSSSPA 1723
>XP_006723815
          Length = 2051

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    NALNSEPA 9
            NAL+S PA
Sbjct: 1704 NALSSSPA 1711
>XP_006723814
          Length = 2052

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    NALNSEPA 9
            NAL+S PA
Sbjct: 1705 NALSSSPA 1712
>XP_006723813
          Length = 2062

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    NALNSEPA 9
            NAL+S PA
Sbjct: 1716 NALSSSPA 1723
>XP_005260405
          Length = 2063

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    NALNSEPA 9
            NAL+S PA
Sbjct: 1716 NALSSSPA 1723
>NP_001094058
          Length = 1191

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   NALNSEP 8
           NA++SEP
Sbjct: 849 NAIDSEP 855
>NP_061323
          Length = 1064

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   NALNSEP 8
           NA++SEP
Sbjct: 849 NAIDSEP 855
>XP_006716433
          Length = 3069

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   NALNSE 7
           NALNSE
Sbjct: 223 NALNSE 228
>XP_005273633
          Length = 3069

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   NALNSE 7
           NALNSE
Sbjct: 223 NALNSE 228
>XP_006716432
          Length = 3172

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   NALNSE 7
           NALNSE
Sbjct: 326 NALNSE 331
>XP_005273632
          Length = 3172

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   NALNSE 7
           NALNSE
Sbjct: 326 NALNSE 331
>NP_079033
          Length = 1298

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    NALNSEP 8
            NA++SEP
Sbjct: 1027 NAVDSEP 1033
>NP_001275915
          Length = 1616

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    ANALNS 6
            ANALNS
Sbjct: 1261 ANALNS 1266
>NP_001275914
          Length = 1621

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    ANALNS 6
            ANALNS
Sbjct: 1266 ANALNS 1271
>NP_001537
          Length = 161

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   ALNSEPA 9
           ALN++PA
Sbjct: 142 ALNTDPA 148
>NP_009099
          Length = 1581

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    ANALNS 6
            ANALNS
Sbjct: 1226 ANALNS 1231
>NP_005654
          Length = 539

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   ALNSEPA 9
           +LN+EPA
Sbjct: 323 SLNNEPA 329
>NP_056052
          Length = 1354

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   NALNSEP 8
           N+LNS P
Sbjct: 662 NSLNSNP 668
>NP_079467
          Length = 932

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   NALNSEP 8
           N+LNS P
Sbjct: 838 NSLNSHP 844
>NP_001138648
          Length = 538

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  ANALNSEPA 9
          +NA ++EPA
Sbjct: 22 SNATSAEPA 30
>XP_005256997
          Length = 1560

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    ANALNS 6
            ANALNS
Sbjct: 1205 ANALNS 1210
>XP_005256995
          Length = 1621

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    ANALNS 6
            ANALNS
Sbjct: 1266 ANALNS 1271
>XP_006717353
          Length = 1243

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   NALNSEP 8
           NA++SEP
Sbjct: 972 NAVDSEP 978
>XP_006717352
          Length = 1250

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   NALNSEP 8
           NA++SEP
Sbjct: 979 NAVDSEP 985
>XP_005266166
          Length = 1267

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    NALNSEP 8
            NA++SEP
Sbjct: 996  NAVDSEP 1002
>XP_005266167
          Length = 1267

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    NALNSEP 8
            NA++SEP
Sbjct: 996  NAVDSEP 1002
>XP_005266165
          Length = 1272

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    NALNSEP 8
            NA++SEP
Sbjct: 1001 NAVDSEP 1007
>XP_005266164
          Length = 1291

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    NALNSEP 8
            NA++SEP
Sbjct: 1020 NAVDSEP 1026
>XP_006717351
          Length = 1292

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    NALNSEP 8
            NA++SEP
Sbjct: 1021 NAVDSEP 1027
>XP_005266163
          Length = 1294

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    NALNSEP 8
            NA++SEP
Sbjct: 1023 NAVDSEP 1029
>XP_005266162
          Length = 1295

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    NALNSEP 8
            NA++SEP
Sbjct: 1024 NAVDSEP 1030
>XP_006713980
          Length = 458

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   ALNSEPA 9
           +LN+EPA
Sbjct: 242 SLNNEPA 248
>XP_006713979
          Length = 564

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   ALNSEPA 9
           +LN+EPA
Sbjct: 348 SLNNEPA 354
>XP_005245556
          Length = 933

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   NALNSEP 8
           N+LNS P
Sbjct: 839 NSLNSHP 845
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.289    0.105    0.267 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 678,629
Number of extensions: 793
Number of successful extensions: 39
Number of sequences better than 10000.0: 39
Number of HSP's gapped: 39
Number of HSP's successfully gapped: 39
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (14.9 bits)
S2: 28 (15.4 bits)

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