Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000537                                                              20     509
NP_001263689                                                           20     509
NP_001119584                                                           20     509
NP_001119586                                                           20     509
NP_001263625                                                           20     509
NP_001119590                                                           20     509
NP_001263690                                                           20     509
NP_001119585                                                           20     509
NP_001263624                                                           20     509
NP_001119588                                                           20     509
NP_001119587                                                           20     509
NP_001119589                                                           20     509
NP_001263627                                                           18    1482
NP_001263628                                                           18    1482
NP_001263626                                                           18    1482
NP_620055                                                              17    3301
NP_060214                                                              17    3301
NP_620056                                                              17    3301
NP_620057                                                              17    3301
XP_005271027                                                           17    3301
XP_005271023                                                           17    3301
XP_006710772                                                           17    3301
XP_005271021                                                           17    3301
XP_005271020                                                           17    3301
XP_006710771                                                           17    3301
XP_005259653                                                           16    5631
NP_064555                                                              16    5631
NP_061923                                                              16    7355
XP_005263135                                                           16    7355
XP_005263134                                                           16    7355
XP_006714303                                                           16    7355
XP_005263133                                                           16    7355
XP_005263132                                                           16    7355
XP_005263131                                                           16    7355
XP_005263130                                                           16    7355
NP_005319                                                              15    9605
XP_005250117                                                           15    9605
XP_005250100                                                           15    9605
NP_003583                                                              15    9605
>NP_000537
          Length = 393

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   AMTIYKQSQ 9
           AM IYKQSQ
Sbjct: 159 AMAIYKQSQ 167
>NP_001263689
          Length = 354

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   AMTIYKQSQ 9
           AM IYKQSQ
Sbjct: 120 AMAIYKQSQ 128
>NP_001119584
          Length = 393

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   AMTIYKQSQ 9
           AM IYKQSQ
Sbjct: 159 AMAIYKQSQ 167
>NP_001119586
          Length = 341

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   AMTIYKQSQ 9
           AM IYKQSQ
Sbjct: 159 AMAIYKQSQ 167
>NP_001263625
          Length = 302

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   AMTIYKQSQ 9
           AM IYKQSQ
Sbjct: 120 AMAIYKQSQ 128
>NP_001119590
          Length = 354

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   AMTIYKQSQ 9
           AM IYKQSQ
Sbjct: 120 AMAIYKQSQ 128
>NP_001263690
          Length = 354

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   AMTIYKQSQ 9
           AM IYKQSQ
Sbjct: 120 AMAIYKQSQ 128
>NP_001119585
          Length = 346

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   AMTIYKQSQ 9
           AM IYKQSQ
Sbjct: 159 AMAIYKQSQ 167
>NP_001263624
          Length = 307

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   AMTIYKQSQ 9
           AM IYKQSQ
Sbjct: 120 AMAIYKQSQ 128
>NP_001119588
          Length = 209

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  AMTIYKQSQ 9
          AM IYKQSQ
Sbjct: 27 AMAIYKQSQ 35
>NP_001119587
          Length = 261

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  AMTIYKQSQ 9
          AM IYKQSQ
Sbjct: 27 AMAIYKQSQ 35
>NP_001119589
          Length = 214

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  AMTIYKQSQ 9
          AM IYKQSQ
Sbjct: 27 AMAIYKQSQ 35
>NP_001263627
          Length = 182

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2 MTIYKQSQ 9
         M IYKQSQ
Sbjct: 1 MAIYKQSQ 8
>NP_001263628
          Length = 187

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2 MTIYKQSQ 9
         M IYKQSQ
Sbjct: 1 MAIYKQSQ 8
>NP_001263626
          Length = 234

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2 MTIYKQSQ 9
         M IYKQSQ
Sbjct: 1 MAIYKQSQ 8
>NP_620055
          Length = 558

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   TIYKQSQ 9
           TIY+QSQ
Sbjct: 249 TIYRQSQ 255
>NP_060214
          Length = 575

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   TIYKQSQ 9
           TIY+QSQ
Sbjct: 266 TIYRQSQ 272
>NP_620056
          Length = 544

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   TIYKQSQ 9
           TIY+QSQ
Sbjct: 266 TIYRQSQ 272
>NP_620057
          Length = 555

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   TIYKQSQ 9
           TIY+QSQ
Sbjct: 266 TIYRQSQ 272
>XP_005271027
          Length = 319

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   TIYKQSQ 9
           TIY+QSQ
Sbjct: 266 TIYRQSQ 272
>XP_005271023
          Length = 418

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   TIYKQSQ 9
           TIY+QSQ
Sbjct: 266 TIYRQSQ 272
>XP_006710772
          Length = 447

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   TIYKQSQ 9
           TIY+QSQ
Sbjct: 266 TIYRQSQ 272
>XP_005271021
          Length = 453

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   TIYKQSQ 9
           TIY+QSQ
Sbjct: 144 TIYRQSQ 150
>XP_005271020
          Length = 453

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   TIYKQSQ 9
           TIY+QSQ
Sbjct: 144 TIYRQSQ 150
>XP_006710771
          Length = 478

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   TIYKQSQ 9
           TIY+QSQ
Sbjct: 266 TIYRQSQ 272
>XP_005259653
          Length = 562

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   MTIYKQSQ 9
           ++IYKQ+Q
Sbjct: 153 LSIYKQTQ 160
>NP_064555
          Length = 563

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   MTIYKQSQ 9
           ++IYKQ+Q
Sbjct: 153 LSIYKQTQ 160
>NP_061923
          Length = 1073

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   AMTIYKQ 7
           AMT+Y+Q
Sbjct: 156 AMTLYEQ 162
>XP_005263135
          Length = 488

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   AMTIYKQ 7
           AMT+Y+Q
Sbjct: 156 AMTLYEQ 162
>XP_005263134
          Length = 800

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   AMTIYKQ 7
           AMT+Y+Q
Sbjct: 156 AMTLYEQ 162
>XP_006714303
          Length = 957

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  AMTIYKQ 7
          AMT+Y+Q
Sbjct: 40 AMTLYEQ 46
>XP_005263133
          Length = 1022

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   AMTIYKQ 7
           AMT+Y+Q
Sbjct: 156 AMTLYEQ 162
>XP_005263132
          Length = 1023

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   AMTIYKQ 7
           AMT+Y+Q
Sbjct: 156 AMTLYEQ 162
>XP_005263131
          Length = 1073

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   AMTIYKQ 7
           AMT+Y+Q
Sbjct: 156 AMTLYEQ 162
>XP_005263130
          Length = 1073

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   AMTIYKQ 7
           AMT+Y+Q
Sbjct: 156 AMTLYEQ 162
>NP_005319
          Length = 552

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   TIYKQSQ 9
           TIYK+S+
Sbjct: 495 TIYKESK 501
>XP_005250117
          Length = 776

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   MTIYKQS 8
           +T+YK+S
Sbjct: 225 LTVYKES 231
>XP_005250100
          Length = 308

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   IYKQSQ 9
           +YKQSQ
Sbjct: 285 LYKQSQ 290
>NP_003583
          Length = 776

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   MTIYKQS 8
           +T+YK+S
Sbjct: 225 LTVYKES 231
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.313    0.113    0.278 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 371,201
Number of extensions: 325
Number of successful extensions: 39
Number of sequences better than 10000.0: 39
Number of HSP's gapped: 39
Number of HSP's successfully gapped: 39
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)

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