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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_733751                                                              19     869
XP_006716142                                                           19     869
XP_005250088                                                           19     869
XP_006716141                                                           19     869
XP_005250085                                                           19     869
XP_006716140                                                           19     869
XP_005250084                                                           19     869
XP_005250083                                                           19     869
XP_005250082                                                           19     869
NP_848547                                                              17    4312
XP_006721193                                                           17    4312
XP_006721192                                                           17    4312
NP_001349                                                              16    7355
NP_056056                                                              16    7355
XP_006724152                                                           16    7355
XP_005261319                                                           16    7355
XP_006724151                                                           16    7355
XP_006724150                                                           16    7355
XP_006724149                                                           16    7355
XP_006724148                                                           16    7355
XP_006724147                                                           16    7355
XP_006724146                                                           16    7355
XP_006724145                                                           16    7355
XP_006724144                                                           16    7355
XP_006723786                                                           16    7355
XP_006723783                                                           16    7355
XP_006723781                                                           16    7355
XP_006723780                                                           16    7355
XP_006723779                                                           16    7355
NP_066554                                                              15    9605
NP_001243404                                                           15    9605
XP_005261676                                                           15    9605
XP_006724320                                                           15    9605

>NP_733751
          Length = 4911

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    AMIRQRSE 8
            AMIRQRSE
Sbjct: 3074 AMIRQRSE 3081



>XP_006716142
          Length = 4927

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    AMIRQRSE 8
            AMIRQRSE
Sbjct: 3091 AMIRQRSE 3098



>XP_005250088
          Length = 4928

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    AMIRQRSE 8
            AMIRQRSE
Sbjct: 3091 AMIRQRSE 3098



>XP_006716141
          Length = 4959

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    AMIRQRSE 8
            AMIRQRSE
Sbjct: 3091 AMIRQRSE 3098



>XP_005250085
          Length = 4979

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    AMIRQRSE 8
            AMIRQRSE
Sbjct: 3091 AMIRQRSE 3098



>XP_006716140
          Length = 4982

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    AMIRQRSE 8
            AMIRQRSE
Sbjct: 3091 AMIRQRSE 3098



>XP_005250084
          Length = 4982

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    AMIRQRSE 8
            AMIRQRSE
Sbjct: 3091 AMIRQRSE 3098



>XP_005250083
          Length = 4982

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    AMIRQRSE 8
            AMIRQRSE
Sbjct: 3090 AMIRQRSE 3097



>XP_005250082
          Length = 4983

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    AMIRQRSE 8
            AMIRQRSE
Sbjct: 3091 AMIRQRSE 3098



>NP_848547
          Length = 725

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   AMIRQRSE 8
           AMI+QR+E
Sbjct: 325 AMIKQRAE 332



>XP_006721193
          Length = 475

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  AMIRQRSE 8
          AMI+QR+E
Sbjct: 73 AMIKQRAE 80



>XP_006721192
          Length = 727

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   AMIRQRSE 8
           AMI+QR+E
Sbjct: 325 AMIKQRAE 332



>NP_001349
          Length = 795

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   MIRQRSEL 9
           ++RQRS+L
Sbjct: 279 VVRQRSDL 286



>NP_056056
          Length = 2002

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    AMIRQRSEL 9
            AM+R  SEL
Sbjct: 1906 AMVRYESEL 1914



>XP_006724152
          Length = 1382

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    AMIRQRSEL 9
            AM+R  SEL
Sbjct: 1286 AMVRYESEL 1294



>XP_005261319
          Length = 2002

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    AMIRQRSEL 9
            AM+R  SEL
Sbjct: 1906 AMVRYESEL 1914



>XP_006724151
          Length = 2063

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    AMIRQRSEL 9
            AM+R  SEL
Sbjct: 1967 AMVRYESEL 1975



>XP_006724150
          Length = 2154

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    AMIRQRSEL 9
            AM+R  SEL
Sbjct: 2058 AMVRYESEL 2066



>XP_006724149
          Length = 2168

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    AMIRQRSEL 9
            AM+R  SEL
Sbjct: 2072 AMVRYESEL 2080



>XP_006724148
          Length = 2176

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    AMIRQRSEL 9
            AM+R  SEL
Sbjct: 2080 AMVRYESEL 2088



>XP_006724147
          Length = 2187

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    AMIRQRSEL 9
            AM+R  SEL
Sbjct: 2091 AMVRYESEL 2099



>XP_006724146
          Length = 2193

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    AMIRQRSEL 9
            AM+R  SEL
Sbjct: 2097 AMVRYESEL 2105



>XP_006724145
          Length = 2210

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    AMIRQRSEL 9
            AM+R  SEL
Sbjct: 2114 AMVRYESEL 2122



>XP_006724144
          Length = 2214

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    AMIRQRSEL 9
            AM+R  SEL
Sbjct: 2118 AMVRYESEL 2126



>XP_006723786
          Length = 355

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   AMIRQRSE 8
           A +RQRSE
Sbjct: 314 ARLRQRSE 321



>XP_006723783
          Length = 410

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   AMIRQRSE 8
           A +RQRSE
Sbjct: 369 ARLRQRSE 376



>XP_006723781
          Length = 485

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   AMIRQRSE 8
           A +RQRSE
Sbjct: 314 ARLRQRSE 321



>XP_006723780
          Length = 517

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   AMIRQRSE 8
           A +RQRSE
Sbjct: 346 ARLRQRSE 353



>XP_006723779
          Length = 540

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   AMIRQRSE 8
           A +RQRSE
Sbjct: 369 ARLRQRSE 376



>NP_066554
          Length = 1020

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   RQRSEL 9
           RQRSEL
Sbjct: 344 RQRSEL 349



>NP_001243404
          Length = 465

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   RQRSEL 9
           RQRSEL
Sbjct: 360 RQRSEL 365



>XP_005261676
          Length = 986

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   RQRSEL 9
           RQRSEL
Sbjct: 344 RQRSEL 349



>XP_006724320
          Length = 1006

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   RQRSEL 9
           RQRSEL
Sbjct: 344 RQRSEL 349



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.329    0.130    0.306 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 328,727
Number of extensions: 345
Number of successful extensions: 33
Number of sequences better than 10000.0: 33
Number of HSP's gapped: 33
Number of HSP's successfully gapped: 33
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)