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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004110                                                              18    1935
NP_005202                                                              16    5631
NP_006197                                                              16    5631
NP_002600                                                              16    5631
NP_001275634                                                           16    5631
XP_005268521                                                           16    5631
XP_006714104                                                           16    5631
XP_005265800                                                           16    5631
XP_006714103                                                           16    5631
XP_006714102                                                           16    5631
NP_001175                                                              16    7355

>NP_004110
          Length = 993

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ALMSVLKMM 9
           ALMS LKMM
Sbjct: 657 ALMSELKMM 665



>NP_005202
          Length = 972

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   ALMSVLKMM 9
           ALMS LK+M
Sbjct: 629 ALMSELKIM 637



>NP_006197
          Length = 1089

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   ALMSVLKMM 9
           ALMS LK+M
Sbjct: 640 ALMSELKIM 648



>NP_002600
          Length = 1106

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   ALMSVLKMM 9
           ALMS LK+M
Sbjct: 647 ALMSELKIM 655



>NP_001275634
          Length = 972

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   ALMSVLKMM 9
           ALMS LK+M
Sbjct: 629 ALMSELKIM 637



>XP_005268521
          Length = 1042

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   ALMSVLKMM 9
           ALMS LK+M
Sbjct: 583 ALMSELKIM 591



>XP_006714104
          Length = 832

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   ALMSVLKMM 9
           ALMS LK+M
Sbjct: 665 ALMSELKIM 673



>XP_005265800
          Length = 1089

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   ALMSVLKMM 9
           ALMS LK+M
Sbjct: 640 ALMSELKIM 648



>XP_006714103
          Length = 1111

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   ALMSVLKMM 9
           ALMS LK+M
Sbjct: 662 ALMSELKIM 670



>XP_006714102
          Length = 1114

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   ALMSVLKMM 9
           ALMS LK+M
Sbjct: 665 ALMSELKIM 673



>NP_001175
          Length = 2644

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1    ALMSVLKMM 9
            +LMS++K+M
Sbjct: 1154 SLMSLMKLM 1162



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.342    0.132    0.302 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 172,515
Number of extensions: 92
Number of successful extensions: 11
Number of sequences better than 10000.0: 11
Number of HSP's gapped: 11
Number of HSP's successfully gapped: 11
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.7 bits)
S2: 28 (15.4 bits)