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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_063946                                                              17    3301
NP_001137446                                                           17    3301
NP_001035902                                                           17    4312
NP_003887                                                              17    4312
XP_005264687                                                           17    4312
XP_006712190                                                           17    4312
NP_055273                                                              16    5631
NP_775960                                                              16    5631
XP_005251991                                                           16    5631
XP_005245150                                                           16    5631
XP_006711340                                                           16    5631
NP_001180211                                                           16    7355
NP_001182058                                                           16    7355
NP_001054                                                              16    7355
NP_001092805                                                           16    7355
NP_055574                                                              16    7355
NP_001137246                                                           16    7355
XP_005270285                                                           16    7355
XP_005270284                                                           16    7355
XP_006716479                                                           16    7355
XP_006713948                                                           16    7355
NP_115815                                                              16    7355
NP_002323                                                              15    9605
NP_060762                                                              15    9605
NP_001230655                                                           15    9605
NP_001230654                                                           15    9605
NP_001123356                                                           15    9605
NP_055429                                                              15    9605
NP_001265124                                                           15    9605
NP_078905                                                              15    9605
NP_057010                                                              15    9605
XP_005263275                                                           15    9605
XP_006713261                                                           15    9605
XP_006713260                                                           15    9605
XP_006713259                                                           15    9605
XP_006713258                                                           15    9605

>NP_063946
          Length = 780

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   AKTCRAAVG 9
           +KTC+ A+G
Sbjct: 445 SKTCKPALG 453



>NP_001137446
          Length = 745

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   AKTCRAAVG 9
           +KTC+ A+G
Sbjct: 410 SKTCKPALG 418



>NP_001035902
          Length = 395

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   AKTCRAAV 8
           AKTCR  V
Sbjct: 166 AKTCRRCV 173



>NP_003887
          Length = 418

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   AKTCRAAV 8
           AKTCR  V
Sbjct: 189 AKTCRRCV 196



>XP_005264687
          Length = 365

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   AKTCRAAV 8
           AKTCR  V
Sbjct: 189 AKTCRRCV 196



>XP_006712190
          Length = 378

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   AKTCRAAV 8
           AKTCR  V
Sbjct: 149 AKTCRRCV 156



>NP_055273
          Length = 609

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   TCRAAVG 9
           TCR +VG
Sbjct: 401 TCRTSVG 407



>NP_775960
          Length = 1285

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    TCRAAVG 9
            TCRAA G
Sbjct: 1187 TCRAAGG 1193



>XP_005251991
          Length = 953

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   TCRAAVG 9
           TCRAA G
Sbjct: 855 TCRAAGG 861



>XP_005245150
          Length = 609

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   TCRAAVG 9
           TCR +VG
Sbjct: 401 TCRTSVG 407



>XP_006711340
          Length = 684

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   TCRAAVG 9
           TCR +VG
Sbjct: 476 TCRTSVG 482



>NP_001180211
          Length = 646

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   CRAAVG 9
           CRAA+G
Sbjct: 543 CRAAMG 548



>NP_001182058
          Length = 603

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   TCRAAVG 9
           TCR A+G
Sbjct: 255 TCRRALG 261



>NP_001054
          Length = 698

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   KTCRAAVG 9
           K+C  AVG
Sbjct: 467 KSCHTAVG 474



>NP_001092805
          Length = 330

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   KTCRAAVG 9
           KTC  +VG
Sbjct: 322 KTCNISVG 329



>NP_055574
          Length = 330

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   KTCRAAVG 9
           KTC  +VG
Sbjct: 322 KTCNISVG 329



>NP_001137246
          Length = 350

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   KTCRAAVG 9
           KTC  +VG
Sbjct: 342 KTCNISVG 349



>XP_005270285
          Length = 330

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   KTCRAAVG 9
           KTC  +VG
Sbjct: 322 KTCNISVG 329



>XP_005270284
          Length = 330

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   KTCRAAVG 9
           KTC  +VG
Sbjct: 322 KTCNISVG 329



>XP_006716479
          Length = 330

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   KTCRAAVG 9
           KTC  +VG
Sbjct: 322 KTCNISVG 329



>XP_006713948
          Length = 747

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   CRAAVG 9
           CRAA+G
Sbjct: 544 CRAAMG 549



>NP_115815
          Length = 376

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   KTCRAAVG 9
           KTC  +VG
Sbjct: 368 KTCNISVG 375



>NP_002323
          Length = 4544

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   AKTCRAAVG 9
           A+TCR   G
Sbjct: 501 ARTCRCRSG 509



>NP_060762
          Length = 528

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   AKTCRAAVG 9
           A TC AA+G
Sbjct: 181 AFTCYAALG 189



>NP_001230655
          Length = 313

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4  CRAAVG 9
          CR AVG
Sbjct: 92 CRCAVG 97



>NP_001230654
          Length = 313

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4  CRAAVG 9
          CR AVG
Sbjct: 92 CRCAVG 97



>NP_001123356
          Length = 313

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4  CRAAVG 9
          CR AVG
Sbjct: 92 CRCAVG 97



>NP_055429
          Length = 1843

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   AKTCRAAV 8
           A  CRAAV
Sbjct: 628 ADMCRAAV 635



>NP_001265124
          Length = 313

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4  CRAAVG 9
          CR AVG
Sbjct: 92 CRCAVG 97



>NP_078905
          Length = 418

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   AKTCRAAV 8
           +K C+AA+
Sbjct: 271 SKVCKAAI 278



>NP_057010
          Length = 313

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4  CRAAVG 9
          CR AVG
Sbjct: 92 CRCAVG 97



>XP_005263275
          Length = 389

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   AKTCRAAV 8
           +K C+AA+
Sbjct: 242 SKVCKAAI 249



>XP_006713261
          Length = 242

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4  CRAAVG 9
          CR AVG
Sbjct: 21 CRCAVG 26



>XP_006713260
          Length = 313

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4  CRAAVG 9
          CR AVG
Sbjct: 92 CRCAVG 97



>XP_006713259
          Length = 313

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4  CRAAVG 9
          CR AVG
Sbjct: 92 CRCAVG 97



>XP_006713258
          Length = 313

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4  CRAAVG 9
          CR AVG
Sbjct: 92 CRCAVG 97



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.324    0.124    0.385 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 508,865
Number of extensions: 512
Number of successful extensions: 36
Number of sequences better than 10000.0: 36
Number of HSP's gapped: 36
Number of HSP's successfully gapped: 36
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)