Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              22     103
NP_001075109                                                           22     103
XP_005272075                                                           18    1935
NP_057688                                                              18    1935
NP_003842                                                              17    4312
NP_006166                                                              17    4312
NP_001248392                                                           17    4312
NP_932326                                                              17    4312
XP_005269924                                                           17    4312
XP_005269922                                                           17    4312
XP_006717933                                                           17    4312
XP_005269921                                                           17    4312
NP_060617                                                              16    5631
NP_000568                                                              16    5631
NP_776214                                                              16    5631
NP_776213                                                              16    5631
NP_001094385                                                           16    5631
NP_001028204                                                           16    5631
NP_001028203                                                           16    5631
NP_001028202                                                           16    5631
XP_005255865                                                           16    5631
XP_006721206                                                           16    5631
NP_689961                                                              16    7355
NP_001136221                                                           16    7355
NP_116202                                                              16    7355
NP_112600                                                              16    7355
NP_001073137                                                           16    7355
NP_001273382                                                           16    7355
XP_006720018                                                           16    7355
XP_005254139                                                           16    7355
XP_006720017                                                           16    7355
XP_006715958                                                           16    7355
XP_006715957                                                           16    7355
NP_620595                                                              15    9605
NP_620594                                                              15    9605
NP_620596                                                              15    9605
NP_005547                                                              15    9605
NP_004035                                                              15    9605
NP_001041                                                              15    9605
NP_005081                                                              15    9605
NP_001185517                                                           15    9605
NP_001158058                                                           15    9605
XP_006712608                                                           15    9605
XP_006711681                                                           15    9605
XP_006711680                                                           15    9605
XP_005245637                                                           15    9605
NP_001108104                                                           15    9605
>NP_008835
          Length = 4128

 Score = 21.9 bits (45), Expect =   103
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    AKMDAMNIW 9
            AKMD MNIW
Sbjct: 3171 AKMDPMNIW 3179
>NP_001075109
          Length = 4097

 Score = 21.9 bits (45), Expect =   103
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    AKMDAMNIW 9
            AKMD MNIW
Sbjct: 3171 AKMDPMNIW 3179
>XP_005272075
          Length = 1561

 Score = 17.7 bits (34), Expect =  1935
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   DAMNIW 9
           DAMN+W
Sbjct: 767 DAMNLW 772
>NP_057688
          Length = 1761

 Score = 17.7 bits (34), Expect =  1935
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   DAMNIW 9
           DAMN+W
Sbjct: 967 DAMNLW 972
>NP_003842
          Length = 220

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   AKMDAMNIW 9
           AK++  NIW
Sbjct: 190 AKLNITNIW 198
>NP_006166
          Length = 1695

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   AKMDAMNI 8
           AK++AMNI
Sbjct: 982 AKLNAMNI 989
>NP_001248392
          Length = 1731

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    AKMDAMNI 8
            AK++AMNI
Sbjct: 1017 AKLNAMNI 1024
>NP_932326
          Length = 1730

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    AKMDAMNI 8
            AK++AMNI
Sbjct: 1017 AKLNAMNI 1024
>XP_005269924
          Length = 1627

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    AKMDAMNI 8
            AK++AMNI
Sbjct: 1017 AKLNAMNI 1024
>XP_005269922
          Length = 1695

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   AKMDAMNI 8
           AK++AMNI
Sbjct: 981 AKLNAMNI 988
>XP_006717933
          Length = 1696

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   AKMDAMNI 8
           AK++AMNI
Sbjct: 982 AKLNAMNI 989
>XP_005269921
          Length = 1696

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   AKMDAMNI 8
           AK++AMNI
Sbjct: 982 AKLNAMNI 989
>NP_060617
          Length = 179

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 4   DAMNIW 9
           DAM++W
Sbjct: 116 DAMDVW 121
>NP_000568
          Length = 159

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1  AKMDAMNIW 9
          +KM A  IW
Sbjct: 15 SKMQAFRIW 23
>NP_776214
          Length = 177

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1  AKMDAMNIW 9
          +KM A  IW
Sbjct: 33 SKMQAFRIW 41
>NP_776213
          Length = 180

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1  AKMDAMNIW 9
          +KM A  IW
Sbjct: 36 SKMQAFRIW 44
>NP_001094385
          Length = 926

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   AKMDAMNIW 9
           A MD +N W
Sbjct: 377 APMDVLNEW 385
>NP_001028204
          Length = 179

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 4   DAMNIW 9
           DAM++W
Sbjct: 116 DAMDVW 121
>NP_001028203
          Length = 201

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 4   DAMNIW 9
           DAM++W
Sbjct: 138 DAMDVW 143
>NP_001028202
          Length = 213

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 4   DAMNIW 9
           DAM++W
Sbjct: 150 DAMDVW 155
>XP_005255865
          Length = 917

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   AKMDAMNIW 9
           A MD +N W
Sbjct: 377 APMDVLNEW 385
>XP_006721206
          Length = 921

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   AKMDAMNIW 9
           A MD +N W
Sbjct: 372 APMDVLNEW 380
>NP_689961
          Length = 862

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   AKMDAMNIW 9
           +K D MN W
Sbjct: 293 SKCDGMNGW 301
>NP_001136221
          Length = 1029

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   AKMDAMNIW 9
           +K D MN W
Sbjct: 460 SKCDGMNGW 468
>NP_116202
          Length = 760

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 3   MDAMNIW 9
           +DA+N W
Sbjct: 620 VDALNAW 626
>NP_112600
          Length = 442

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 2   KMDAMNIW 9
           +MD  N+W
Sbjct: 234 EMDLRNLW 241
>NP_001073137
          Length = 741

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 3   MDAMNIW 9
           +DA+N W
Sbjct: 601 VDALNAW 607
>NP_001273382
          Length = 630

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 3   MDAMNIW 9
           +DA+N W
Sbjct: 490 VDALNAW 496
>XP_006720018
          Length = 514

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 3   MDAMNIW 9
           +DA+N W
Sbjct: 374 VDALNAW 380
>XP_005254139
          Length = 524

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 3   MDAMNIW 9
           +DA+N W
Sbjct: 384 VDALNAW 390
>XP_006720017
          Length = 741

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 3   MDAMNIW 9
           +DA+N W
Sbjct: 601 VDALNAW 607
>XP_006715958
          Length = 915

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   AKMDAMNIW 9
           +K D MN W
Sbjct: 346 SKCDGMNGW 354
>XP_006715957
          Length = 915

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   AKMDAMNIW 9
           +K D MN W
Sbjct: 346 SKCDGMNGW 354
>NP_620595
          Length = 1340

 Score = 15.4 bits (28), Expect =  9605
 Identities = 3/8 (37%), Positives = 6/8 (75%)

Query: 2   KMDAMNIW 9
           +MD+  +W
Sbjct: 504 RMDSQQVW 511
>NP_620594
          Length = 1427

 Score = 15.4 bits (28), Expect =  9605
 Identities = 3/8 (37%), Positives = 6/8 (75%)

Query: 2   KMDAMNIW 9
           +MD+  +W
Sbjct: 535 RMDSQQVW 542
>NP_620596
          Length = 1371

 Score = 15.4 bits (28), Expect =  9605
 Identities = 3/8 (37%), Positives = 6/8 (75%)

Query: 2   KMDAMNIW 9
           +MD+  +W
Sbjct: 535 RMDSQQVW 542
>NP_005547
          Length = 469

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   AKMDAMN 7
           AK+DA+N
Sbjct: 213 AKVDALN 219
>NP_004035
          Length = 592

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 5/6 (83%)

Query: 4   DAMNIW 9
           DA+N W
Sbjct: 242 DALNAW 247
>NP_001041
          Length = 369

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   AKMDAMNIW 9
           AKM  M +W
Sbjct: 159 AKMITMAVW 167
>NP_005081
          Length = 1012

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 5/6 (83%)

Query: 4   DAMNIW 9
           DA+N W
Sbjct: 162 DAVNFW 167
>NP_001185517
          Length = 893

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 5/6 (83%)

Query: 4   DAMNIW 9
           DA+N W
Sbjct: 162 DAVNFW 167
>NP_001158058
          Length = 3708

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 5/8 (62%)

Query: 2   KMDAMNIW 9
           KMD   IW
Sbjct: 161 KMDLEQIW 168
>XP_006712608
          Length = 533

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 5/6 (83%)

Query: 4   DAMNIW 9
           DA+N W
Sbjct: 183 DALNAW 188
>XP_006711681
          Length = 3448

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 5/8 (62%)

Query: 2   KMDAMNIW 9
           KMD   IW
Sbjct: 161 KMDLEQIW 168
>XP_006711680
          Length = 3617

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 5/8 (62%)

Query: 2   KMDAMNIW 9
           KMD   IW
Sbjct: 161 KMDLEQIW 168
>XP_005245637
          Length = 3683

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 5/8 (62%)

Query: 2   KMDAMNIW 9
           KMD   IW
Sbjct: 161 KMDLEQIW 168
>NP_001108104
          Length = 316

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 5/6 (83%)

Query: 4   DAMNIW 9
           DA+N W
Sbjct: 162 DAVNFW 167
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.325    0.123    0.447 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 398,824
Number of extensions: 720
Number of successful extensions: 47
Number of sequences better than 10000.0: 47
Number of HSP's gapped: 47
Number of HSP's successfully gapped: 47
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)

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