Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

• Home Page
• Cancer-specific
• Epitope Search
• Data Retrieval
• Browse Gene
• Browse Tissue
• Sequence Similarity
• Partially Personalized
• Submit a Protein
• Vaccine Target
• Proteome Data
• NGS data
• BLAST & Predict
• Fully Personalized
• Proteins Pair
• Proteome Pair
• Advanced Tools
• Epitope Mapping
• Cross Reactivity
• Important Information
• Acknowledgement
• Algorithm
• Help
• Developers
• Contact

Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005148                                                              19     869
NP_009297                                                              19     869
NP_001271141                                                           17    4312
NP_001239264                                                           17    4312
NP_001271142                                                           17    4312
NP_775083                                                              17    4312
NP_001035905                                                           17    4312
NP_001177371                                                           17    4312
NP_116255                                                              17    4312
XP_005254784                                                           17    4312
XP_005254783                                                           17    4312
XP_006714775                                                           17    4312
XP_006714774                                                           17    4312
XP_006714773                                                           17    4312
XP_006714772                                                           17    4312
XP_006714771                                                           17    4312
XP_006714770                                                           17    4312
XP_006714769                                                           17    4312
XP_006714768                                                           17    4312
XP_006714767                                                           17    4312
XP_006714766                                                           17    4312
XP_006714765                                                           17    4312
XP_006714764                                                           17    4312
XP_006714763                                                           17    4312
XP_006714762                                                           17    4312
XP_006714761                                                           17    4312
XP_006714760                                                           17    4312
XP_006714759                                                           17    4312
NP_689914                                                              16    7355
NP_076985                                                              15    9605

>NP_005148
          Length = 1130

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    AISRSSEQM 9
            AISR+SEQM
Sbjct: 1042 AISRNSEQM 1050



>NP_009297
          Length = 1149

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    AISRSSEQM 9
            AISR+SEQM
Sbjct: 1061 AISRNSEQM 1069



>NP_001271141
          Length = 666

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  AISRSSEQ 8
          A+SRS+EQ
Sbjct: 42 AVSRSAEQ 49



>NP_001239264
          Length = 1302

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    ISRSSEQM 9
            ISRS EQM
Sbjct: 1091 ISRSREQM 1098



>NP_001271142
          Length = 636

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  AISRSSEQ 8
          A+SRS+EQ
Sbjct: 61 AVSRSAEQ 68



>NP_775083
          Length = 686

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  AISRSSEQ 8
          A+SRS+EQ
Sbjct: 61 AVSRSAEQ 68



>NP_001035905
          Length = 750

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   AISRSSEQ 8
           A+SRS+EQ
Sbjct: 125 AVSRSAEQ 132



>NP_001177371
          Length = 695

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  AISRSSEQ 8
          A+SRS+EQ
Sbjct: 83 AVSRSAEQ 90



>NP_116255
          Length = 1302

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    ISRSSEQM 9
            ISRS EQM
Sbjct: 1091 ISRSREQM 1098



>XP_005254784
          Length = 1303

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    ISRSSEQM 9
            ISRS EQM
Sbjct: 1091 ISRSREQM 1098



>XP_005254783
          Length = 1303

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    ISRSSEQM 9
            ISRS EQM
Sbjct: 1091 ISRSREQM 1098



>XP_006714775
          Length = 654

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  AISRSSEQ 8
          A+SRS+EQ
Sbjct: 42 AVSRSAEQ 49



>XP_006714774
          Length = 667

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  AISRSSEQ 8
          A+SRS+EQ
Sbjct: 42 AVSRSAEQ 49



>XP_006714773
          Length = 673

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  AISRSSEQ 8
          A+SRS+EQ
Sbjct: 61 AVSRSAEQ 68



>XP_006714772
          Length = 689

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  AISRSSEQ 8
          A+SRS+EQ
Sbjct: 64 AVSRSAEQ 71



>XP_006714771
          Length = 695

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  AISRSSEQ 8
          A+SRS+EQ
Sbjct: 83 AVSRSAEQ 90



>XP_006714770
          Length = 708

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  AISRSSEQ 8
          A+SRS+EQ
Sbjct: 83 AVSRSAEQ 90



>XP_006714769
          Length = 735

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   AISRSSEQ 8
           A+SRS+EQ
Sbjct: 110 AVSRSAEQ 117



>XP_006714768
          Length = 749

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   AISRSSEQ 8
           A+SRS+EQ
Sbjct: 125 AVSRSAEQ 132



>XP_006714767
          Length = 754

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   AISRSSEQ 8
           A+SRS+EQ
Sbjct: 129 AVSRSAEQ 136



>XP_006714766
          Length = 756

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   AISRSSEQ 8
           A+SRS+EQ
Sbjct: 144 AVSRSAEQ 151



>XP_006714765
          Length = 757

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   AISRSSEQ 8
           A+SRS+EQ
Sbjct: 132 AVSRSAEQ 139



>XP_006714764
          Length = 769

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   AISRSSEQ 8
           A+SRS+EQ
Sbjct: 144 AVSRSAEQ 151



>XP_006714763
          Length = 772

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   AISRSSEQ 8
           A+SRS+EQ
Sbjct: 147 AVSRSAEQ 154



>XP_006714762
          Length = 776

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   AISRSSEQ 8
           A+SRS+EQ
Sbjct: 151 AVSRSAEQ 158



>XP_006714761
          Length = 778

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   AISRSSEQ 8
           A+SRS+EQ
Sbjct: 166 AVSRSAEQ 173



>XP_006714760
          Length = 790

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   AISRSSEQ 8
           A+SRS+EQ
Sbjct: 166 AVSRSAEQ 173



>XP_006714759
          Length = 791

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   AISRSSEQ 8
           A+SRS+EQ
Sbjct: 166 AVSRSAEQ 173



>NP_689914
          Length = 5058

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    AISRSSEQ 8
            AISR+SE+
Sbjct: 2481 AISRASEE 2488



>NP_076985
          Length = 1104

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   ISRSSEQ 8
           ISRSSE+
Sbjct: 201 ISRSSEE 207



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.298    0.102    0.214 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 286,505
Number of extensions: 302
Number of successful extensions: 30
Number of sequences better than 10000.0: 30
Number of HSP's gapped: 30
Number of HSP's successfully gapped: 30
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.3 bits)
S2: 28 (15.4 bits)