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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_065715                                                              17    4312
NP_001338                                                              16    7355
NP_001257821                                                           16    7355
NP_001013757                                                           16    7355
NP_078961                                                              15    9605
NP_689622                                                              15    9605
XP_005271947                                                           15    9605
XP_005271946                                                           15    9605
NP_001129058                                                           15    9605
NP_002144                                                              15    9605

>NP_065715
          Length = 292

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   AGSTKAAV 8
           AGS KAAV
Sbjct: 178 AGSAKAAV 185



>NP_001338
          Length = 942

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   AGSTKAAV 8
           AG+TKA V
Sbjct: 522 AGATKATV 529



>NP_001257821
          Length = 127

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  AGSTKAA 7
          AG+TKAA
Sbjct: 38 AGATKAA 44



>NP_001013757
          Length = 432

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   AGSTKAA 7
           AGSTK+A
Sbjct: 121 AGSTKSA 127



>NP_078961
          Length = 723

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  AGSTKAAV 8
          AGS KAA+
Sbjct: 8  AGSVKAAL 15



>NP_689622
          Length = 188

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   GSTKAAVF 9
           G+TKAA F
Sbjct: 111 GTTKAAGF 118



>XP_005271947
          Length = 155

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   GSTKAAVF 9
           G+TKAA F
Sbjct: 111 GTTKAAGF 118



>XP_005271946
          Length = 188

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   GSTKAAVF 9
           G+TKAA F
Sbjct: 111 GTTKAAGF 118



>NP_001129058
          Length = 188

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   GSTKAAVF 9
           G+TKAA F
Sbjct: 111 GTTKAAGF 118



>NP_002144
          Length = 387

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   GSTKAAV 8
           GS+KAAV
Sbjct: 233 GSSKAAV 239



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.313    0.117    0.301 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 351,239
Number of extensions: 381
Number of successful extensions: 10
Number of sequences better than 10000.0: 10
Number of HSP's gapped: 10
Number of HSP's successfully gapped: 10
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)