Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_958441                                                              20     509
NP_958439                                                              20     509
NP_958440                                                              20     509
NP_005219                                                              20     509
XP_006726843                                                           17    3301
XP_006726842                                                           17    3301
XP_006726841                                                           17    3301
XP_005259508                                                           17    3301
XP_006723586                                                           17    3301
XP_006723585                                                           17    3301
XP_006710113                                                           17    3301
XP_003960942                                                           17    3301
XP_006710112                                                           17    3301
XP_005275906                                                           17    3301
NP_057078                                                              16    5631
NP_000910                                                              16    5631
NP_001008744                                                           16    5631
NP_060789                                                              16    5631
XP_006720264                                                           16    5631
XP_006720263                                                           16    5631
XP_006720262                                                           16    5631
XP_006720261                                                           16    5631
XP_005267905                                                           16    5631
XP_005267904                                                           16    5631
XP_006720260                                                           16    5631
XP_006714702                                                           16    5631
XP_006714698                                                           16    5631
XP_006714696                                                           16    5631
XP_006714695                                                           16    5631
NP_002622                                                              16    5631
NP_005769                                                              15    9605
NP_001243224                                                           15    9605
NP_005230                                                              15    9605
NP_001171809                                                           15    9605
NP_689914                                                              15    9605
NP_001098713                                                           15    9605
NP_065817                                                              15    9605
NP_001244896                                                           15    9605
NP_001244897                                                           15    9605
NP_149107                                                              15    9605
NP_919276                                                              15    9605
XP_006724993                                                           15    9605
XP_006724986                                                           15    9605
XP_006724985                                                           15    9605
XP_006724984                                                           15    9605
XP_006724983                                                           15    9605
XP_006724982                                                           15    9605
XP_006724981                                                           15    9605
XP_006724980                                                           15    9605
XP_006724979                                                           15    9605
XP_006724978                                                           15    9605
XP_006724977                                                           15    9605
XP_006724976                                                           15    9605
XP_006724460                                                           15    9605
XP_005260992                                                           15    9605
XP_005268266                                                           15    9605
XP_006720366                                                           15    9605
XP_005268265                                                           15    9605
XP_006719239                                                           15    9605
XP_005249718                                                           15    9605
XP_006712983                                                           15    9605
XP_006712982                                                           15    9605
XP_006712981                                                           15    9605
XP_006712980                                                           15    9605
>NP_958441
          Length = 705

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   AFGADSYEM 9
           A GADSYEM
Sbjct: 310 ACGADSYEM 318
>NP_958439
          Length = 628

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   AFGADSYEM 9
           A GADSYEM
Sbjct: 310 ACGADSYEM 318
>NP_958440
          Length = 405

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   AFGADSYEM 9
           A GADSYEM
Sbjct: 310 ACGADSYEM 318
>NP_005219
          Length = 1210

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   AFGADSYEM 9
           A GADSYEM
Sbjct: 310 ACGADSYEM 318
>XP_006726843
          Length = 718

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   AFGADSY 7
           AFG DSY
Sbjct: 642 AFGRDSY 648
>XP_006726842
          Length = 718

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   AFGADSY 7
           AFG DSY
Sbjct: 642 AFGRDSY 648
>XP_006726841
          Length = 718

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   AFGADSY 7
           AFG DSY
Sbjct: 642 AFGRDSY 648
>XP_005259508
          Length = 718

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   AFGADSY 7
           AFG DSY
Sbjct: 642 AFGRDSY 648
>XP_006723586
          Length = 718

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   AFGADSY 7
           AFG DSY
Sbjct: 642 AFGRDSY 648
>XP_006723585
          Length = 718

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   AFGADSY 7
           AFG DSY
Sbjct: 642 AFGRDSY 648
>XP_006710113
          Length = 742

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   AFGADSY 7
           AFG DSY
Sbjct: 666 AFGRDSY 672
>XP_003960942
          Length = 718

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   AFGADSY 7
           AFG DSY
Sbjct: 642 AFGRDSY 648
>XP_006710112
          Length = 718

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   AFGADSY 7
           AFG DSY
Sbjct: 642 AFGRDSY 648
>XP_005275906
          Length = 718

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   AFGADSY 7
           AFG DSY
Sbjct: 642 AFGRDSY 648
>NP_057078
          Length = 247

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   GADSYEM 9
           G DSYE+
Sbjct: 115 GTDSYEL 121
>NP_000910
          Length = 974

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   AFGADS 6
           AFGADS
Sbjct: 894 AFGADS 899
>NP_001008744
          Length = 608

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   AFGADSYEM 9
           AFG DS+++
Sbjct: 547 AFGLDSFKV 555
>NP_060789
          Length = 608

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   AFGADSYEM 9
           AFG DS+++
Sbjct: 547 AFGLDSFKV 555
>XP_006720264
          Length = 343

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   AFGADSYEM 9
           AFG DS+++
Sbjct: 282 AFGLDSFKV 290
>XP_006720263
          Length = 343

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   AFGADSYEM 9
           AFG DS+++
Sbjct: 282 AFGLDSFKV 290
>XP_006720262
          Length = 343

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   AFGADSYEM 9
           AFG DS+++
Sbjct: 282 AFGLDSFKV 290
>XP_006720261
          Length = 608

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   AFGADSYEM 9
           AFG DS+++
Sbjct: 547 AFGLDSFKV 555
>XP_005267905
          Length = 608

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   AFGADSYEM 9
           AFG DS+++
Sbjct: 547 AFGLDSFKV 555
>XP_005267904
          Length = 608

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   AFGADSYEM 9
           AFG DS+++
Sbjct: 547 AFGLDSFKV 555
>XP_006720260
          Length = 608

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   AFGADSYEM 9
           AFG DS+++
Sbjct: 547 AFGLDSFKV 555
>XP_006714702
          Length = 867

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   AFGADS 6
           AFGADS
Sbjct: 787 AFGADS 792
>XP_006714698
          Length = 973

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   AFGADS 6
           AFGADS
Sbjct: 893 AFGADS 898
>XP_006714696
          Length = 974

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   AFGADS 6
           AFGADS
Sbjct: 894 AFGADS 899
>XP_006714695
          Length = 974

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   AFGADS 6
           AFGADS
Sbjct: 894 AFGADS 899
>NP_002622
          Length = 483

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   FGADSYEM 9
           FGA +YE+
Sbjct: 450 FGAHTYEL 457
>NP_005769
          Length = 815

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   GADSYE 8
           GADSY+
Sbjct: 795 GADSYK 800
>NP_001243224
          Length = 609

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   GADSYE 8
           GADS+E
Sbjct: 422 GADSFE 427
>NP_005230
          Length = 469

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   GADSYE 8
           GADS+E
Sbjct: 282 GADSFE 287
>NP_001171809
          Length = 1148

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   AFGADSYEM 9
           +FG  +YE+
Sbjct: 160 SFGVQTYEL 168
>NP_689914
          Length = 5058

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 2    FGADSY 7
            FG DSY
Sbjct: 4640 FGIDSY 4645
>NP_001098713
          Length = 1101

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   AFGADSYEM 9
           +FG  +YE+
Sbjct: 160 SFGVQTYEL 168
>NP_065817
          Length = 1100

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   AFGADSYEM 9
           +FG  +YE+
Sbjct: 160 SFGVQTYEL 168
>NP_001244896
          Length = 1054

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   GADSYEM 9
           G DS+EM
Sbjct: 392 GPDSFEM 398
>NP_001244897
          Length = 1062

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   GADSYEM 9
           G DS+EM
Sbjct: 408 GPDSFEM 414
>NP_149107
          Length = 979

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   AFGADSY 7
           AFG+D Y
Sbjct: 449 AFGSDYY 455
>NP_919276
          Length = 1044

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   GADSYEM 9
           G DS+EM
Sbjct: 382 GPDSFEM 388
>XP_006724993
          Length = 569

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   ADSYEM 9
           AD+YEM
Sbjct: 214 ADTYEM 219
>XP_006724986
          Length = 488

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   ADSYEM 9
           AD+YEM
Sbjct: 106 ADTYEM 111
>XP_006724985
          Length = 496

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   ADSYEM 9
           AD+YEM
Sbjct: 114 ADTYEM 119
>XP_006724984
          Length = 517

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   ADSYEM 9
           AD+YEM
Sbjct: 135 ADTYEM 140
>XP_006724983
          Length = 525

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   ADSYEM 9
           AD+YEM
Sbjct: 183 ADTYEM 188
>XP_006724982
          Length = 538

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   ADSYEM 9
           AD+YEM
Sbjct: 183 ADTYEM 188
>XP_006724981
          Length = 565

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   ADSYEM 9
           AD+YEM
Sbjct: 183 ADTYEM 188
>XP_006724980
          Length = 565

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   ADSYEM 9
           AD+YEM
Sbjct: 183 ADTYEM 188
>XP_006724979
          Length = 565

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   ADSYEM 9
           AD+YEM
Sbjct: 183 ADTYEM 188
>XP_006724978
          Length = 565

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   ADSYEM 9
           AD+YEM
Sbjct: 183 ADTYEM 188
>XP_006724977
          Length = 565

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   ADSYEM 9
           AD+YEM
Sbjct: 183 ADTYEM 188
>XP_006724976
          Length = 565

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   ADSYEM 9
           AD+YEM
Sbjct: 183 ADTYEM 188
>XP_006724460
          Length = 574

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   ADSYEM 9
           AD+YEM
Sbjct: 219 ADTYEM 224
>XP_005260992
          Length = 469

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   GADSYE 8
           GADS+E
Sbjct: 282 GADSFE 287
>XP_005268266
          Length = 873

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   AFGADSY 7
           AFG+D Y
Sbjct: 331 AFGSDYY 337
>XP_006720366
          Length = 971

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   AFGADSY 7
           AFG+D Y
Sbjct: 449 AFGSDYY 455
>XP_005268265
          Length = 991

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   AFGADSY 7
           AFG+D Y
Sbjct: 449 AFGSDYY 455
>XP_006719239
          Length = 1066

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 2   FGADSYEM 9
           FG D Y++
Sbjct: 178 FGKDGYDL 185
>XP_005249718
          Length = 952

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   GADSYEM 9
           G DS+EM
Sbjct: 290 GPDSFEM 296
>XP_006712983
          Length = 568

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   GADSYE 8
           GADSY+
Sbjct: 548 GADSYK 553
>XP_006712982
          Length = 568

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   GADSYE 8
           GADSY+
Sbjct: 548 GADSYK 553
>XP_006712981
          Length = 568

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   GADSYE 8
           GADSY+
Sbjct: 548 GADSYK 553
>XP_006712980
          Length = 756

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   GADSYE 8
           GADSY+
Sbjct: 736 GADSYK 741
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.317    0.130    0.372 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 624,583
Number of extensions: 1059
Number of successful extensions: 64
Number of sequences better than 10000.0: 64
Number of HSP's gapped: 64
Number of HSP's successfully gapped: 64
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)

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