Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000242                                                              19     665
XP_005264389                                                           19     665
NP_005931                                                              17    4312
NP_056099                                                              16    5631
XP_006726832                                                           16    5631
XP_006722657                                                           16    5631
XP_006710565                                                           16    5631
XP_006710564                                                           16    5631
XP_005270743                                                           16    5631
XP_006710101                                                           16    5631
NP_000278                                                              15    9605
NP_619639                                                              15    9605
NP_733842                                                              15    9605
NP_001007526                                                           15    9605
NP_001277284                                                           15    9605
NP_001135758                                                           15    9605
NP_055838                                                              15    9605
NP_078900                                                              15    9605
NP_001263181                                                           15    9605
XP_005249237                                                           15    9605
XP_006712166                                                           15    9605
XP_005262685                                                           15    9605
>NP_000242
          Length = 934

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  AAEVGLVRF 9
          AAEVG VRF
Sbjct: 14 AAEVGFVRF 22
>XP_005264389
          Length = 921

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  AAEVGLVRF 9
          AAEVG VRF
Sbjct: 14 AAEVGFVRF 22
>NP_005931
          Length = 488

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   EVGLVRF 9
           E+GLVRF
Sbjct: 379 ELGLVRF 385
>NP_056099
          Length = 3375

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    AAEVGLVR 8
            AAEVG+ R
Sbjct: 3195 AAEVGMAR 3202
>XP_006726832
          Length = 223

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  AAEVGLVRF 9
          +A+VG VRF
Sbjct: 59 SAQVGSVRF 67
>XP_006722657
          Length = 223

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  AAEVGLVRF 9
          +A+VG VRF
Sbjct: 59 SAQVGSVRF 67
>XP_006710565
          Length = 3140

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    AAEVGLVR 8
            AAEVG+ R
Sbjct: 2960 AAEVGMAR 2967
>XP_006710564
          Length = 3432

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    AAEVGLVR 8
            AAEVG+ R
Sbjct: 3252 AAEVGMAR 3259
>XP_005270743
          Length = 3453

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    AAEVGLVR 8
            AAEVG+ R
Sbjct: 3273 AAEVGMAR 3280
>XP_006710101
          Length = 223

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  AAEVGLVRF 9
          +A+VG VRF
Sbjct: 59 SAQVGSVRF 67
>NP_000278
          Length = 980

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  AAEVGLV 7
          AAE+GLV
Sbjct: 31 AAELGLV 37
>NP_619639
          Length = 4074

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    AAEVGLV 7
            AAEVGL+
Sbjct: 2947 AAEVGLL 2953
>NP_733842
          Length = 3396

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    AAEVGLV 7
            AAEVGL+
Sbjct: 2947 AAEVGLL 2953
>NP_001007526
          Length = 1432

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   EVGLVR 8
           EVGLVR
Sbjct: 783 EVGLVR 788
>NP_001277284
          Length = 1429

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   EVGLVR 8
           EVGLVR
Sbjct: 648 EVGLVR 653
>NP_001135758
          Length = 1091

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  AAEVGLV 7
          AAEVG++
Sbjct: 42 AAEVGII 48
>NP_055838
          Length = 676

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   AAEVGLVR 8
           AAE GL+R
Sbjct: 153 AAEDGLIR 160
>NP_078900
          Length = 1091

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  AAEVGLV 7
          AAEVG++
Sbjct: 42 AAEVGII 48
>NP_001263181
          Length = 677

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   AAEVGLVR 8
           AAE GL+R
Sbjct: 153 AAEDGLIR 160
>XP_005249237
          Length = 892

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  AAEVGLV 7
          AAE+GLV
Sbjct: 31 AAELGLV 37
>XP_006712166
          Length = 976

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  AAEVGLV 7
          AAEVG++
Sbjct: 64 AAEVGII 70
>XP_005262685
          Length = 1113

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  AAEVGLV 7
          AAEVG++
Sbjct: 64 AAEVGII 70
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.333    0.146    0.393 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 369,807
Number of extensions: 382
Number of successful extensions: 22
Number of sequences better than 10000.0: 22
Number of HSP's gapped: 22
Number of HSP's successfully gapped: 22
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)

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