Fungal infections pose a rising global health threat, particularly among immunocompromised individuals, with common infections like candidiasis and aspergillosis proving difficult to treat. The growing resistance of fungal pathogens to current antifungal drugs, such as azoles and echinocandins, highlights the urgent need for new therapeutic strategies. Metagenomic analysis offers a promising avenue by uncovering genes encoding novel antifungal proteins within diverse microbial communities, presenting potential candidates for next-generation antifungal agents.