## INTERLEUKIN-4 & INTERFERON-gamma DETERMINATION PROGRAM DOCUMENT

The IL4IFNG program is written in GW-BASIC and it can run without math co-processor. IL4-IFN is desigen to estimate the concentration of unknown samples of IL-4 and IFN-$\gamma$ by utilizing the known standard data of these lymphokines. This program is entirely menu driven, follow the menu to run the program. The main menu provides the following five options.

ENTER AND SAVE THE STANDARD DATA: This option allow the users to enter and save the standard data in an ASCII file. The user should enter the new filename when the program ask the file name, for avoiding the overwrite on the old file. The user should enter the IL-4 and IFN-$\gamma$ concentrations and corresponding IgG1 and IgG2a secretion respectively of standard samples (known samples) determined by ELISA method in B cells. The program save the data of standard samples in filename entered by user with extension IL4 and IFN for IL-4 and IFN-$\gamma$ respectively, called the standard data file. This standard data file can be used for the drawing standard curve and for determination the IL-4 concentration.

DRAW THE STANDARD CURVE FROM DATA FILE: This option allow the user to draw a calibration curve by using standard data file.The user should enter the old filename (previously saved standard data file) from directory displayed when the program ask the file name. The program first calculate the usable range (initial portion of the curve with positive slope) of standard data and then fit the standard data in usable range using the least square curve fitting method by draw a calibration curve. You may get a hardcopy with the PrintScrn key to invoke the printer. However, some printer does not support the graph mode. Only the text can be printed out. Under such circumstances, you need to refer to your printer for graphic mode support, or you have to use other screen dump program to print the graph.

CALCULATION OF IL-4 & IFN-gamma CONCENTRATION: This option allow the user to determine the IL-4 \& IFN-$\gamma$ concentration of unknown samples by using there standard data files. The user should enter the old filename (previously saved standard data file) from directory displayed when the program ask the file name. The program uses the least square curve-fitting method by using standard data of file and calculate the IL-4 \& IFN-$\gamma$ concentration of unknown samples corresponding to there IgG1 and IgG2a secretion respectively.

MODIFY THE STANDARD DATA: This option allow the user to edit the standard data of data file. The user should enter the old filename (previously saved standard data file) from directory displayed when the program ask the file name. The program provide the three option to user 1) to add the more data to file. 2) to modify any data of the file 3) to remove the data from file. The program also sort the data and overwrite the new data on old data in same data file.

PRINT THE DATA This option allow the user to print the data of data file on printer. The user should enter the old filename (previously saved standard data file) from directory displayed when the program ask the file name. The program print the IL-4 \& IFN-$\gamma$ concentrations and corresponding peak currents of standard samples respectively.

PLOT STANDARD CURVE The standard curve of IL-4 \& IFN-$\gamma$ can be plot by using HPGL plotter or using HPGL emulsion of the plotter.

Files contained in this package:
FILES DESCRIPTION
IL4IFNG.BAS SOURCE CODE OF IL4IFNG, IN GW-BASIC
PAPER.TEX MANUSCRIPT IN TEX FORMAT (NON-DOCUMENT) FILE
il4ifng.tar All files in tar format
il4ifng.tar.uue uuencoded il4ifng.tar file

LICENCE:

This program remains the property of the Institute of Microbial Technology, Chandigarh, INDIA. This program may be freely used by anybody subject to the following conditions:

1. The authors nor the Institute of Microbial Technology Chandigarh assume any responsibility for any losses or damage that may be caused by the use or misuse of the accompanying software.

2. The authors nor the Institute of Microbial Technology Chandigarh give any warranty with regards to the software being able to function on any computer.

3. The accompanying software may not be copied nor distributed with any modifications, and this document file MUST be included with all copies.

4. No fee may be charged for the copying and/or distribution of the accompanying software.

5. Users must agree to accept any risk as a condition of the free use of the accompanying software.

Any suggestion, bug report or comment will be greatly appreciated. Please send them to:
               G P S Raghava, Scientist

Co-ordinator, Bioinformatics Center

Institute of Microbial Technology,

Sector 39A, Chandigarh, India.