Ab_AFFI 1.0 DOCUMENTATION
Computer program Ab_affi developed for calculating the affinity of monoclonal
antibody from non-comptetitive ELISA data. The Ab_affi is a menu-driven
computer program for calculating the affinity of monoclonal antibody from
non-competitive ELISA data, has been written in GW-BASIC.
OD data obtained from the Microplate reader can be fed to computer either
directly by interfacing or by using Keyboard and data could be stored for
future use. Before starting the calculation, a `dilution template'
should be defined and the information about the serial dilutions of antibody
added to the wells and antigen coated on the plate must also be supplied.
The dilution template consists of arrays of dilution factor (DF),
corresponding to the given [Ag] coated on the assay plate. It calculates the
average OD of samples, which are in duplicate, triplicate, etc.
The computer program, 1) Fits the OD data for given Ag concentration by
hyperbolic curve-fitting method and calculates OD-100, OD-50 and also hyperbolic
interpolation formula; 2) It depicts the Sl-range and calculates the linear
interpolation formula; and 3.) Checks whether OD-50 is in Sl-range and then
calculates accordingly the concentration of [Ab] for a given [Ag]
concentration coated on the plate using equ.~(13) and equ.~(15). In a similar
way the program calculates the [Ab] concentration at OD-50 for other
concentrations of [Ag] coated on the plate. Lastly, it calculates the
antibody affinity by using equ.~(11). It also calculates the capacity and
flatness of hyperbolic curve, which plays a vital role in ELISA optimization.
The program also allows the display and printing of the results. Fig.~1 shows
an example printout from one of such ELISA plate.
Files contained in this package:
|FILES || DESCRIPTION
|AB_AFFI.BAS || SOURCE CODE OF AB_AFFI, IN GW-BASIC
|OD.DAT || EXAMPLE FILE CONTAIN OPTICAL DENSITY DATA
|DT.DAT || EXAMPLE FILE CONTAIN DILUTION TEMPLATE DATA
|PAPER.TEX || MANUSCRIPT IN TEX FORMAT (NON-DOCUMENT) FILE
|abaffi.tar || All files in tar format
|abaffi.tar.uue || uuencoded abaffi.tar file
This program remains the property of the Institute of Microbial Technology,
Chandigarh, INDIA. This program may be freely used by anybody subject to
the following conditions:
1. The authors nor the Institute of Microbial Technology Chandigarh
assume any responsibility for any losses or damage that may be
caused by the use or misuse of the accompanying software.
2. The authors nor the Institute of Microbial Technology Chandigarh give
any warranty with regards to the software being able to function on
3. The accompanying software may not be copied nor distributed with any
modifications, and this document file MUST be included with all copies.
4. No fee may be charged for the copying and/or distribution of the
5. Users must agree to accept any risk as a condition of the free use of
the accompanying software.
Any suggestion, bug report or comment will be greatly appreciated. Please
send them to:
G P S Raghava, Scientist
Co-ordinator, Bioinformatics Center
Institute of Microbial Technology,
Sector 39A, Chandigarh, India.
Email address: email@example.com