ProGlyProt ID BU255 Organism Information Organism Name Pseudomonas putida GR12-2 Domain Bacteria Classification Family: Pseudomonadaceae Order: Pseudomonadales Class: Gammaproteobacteria Division or phylum: "Proteobacteria" Taxonomic ID (NCBI) 303 Genome Sequence (s) GeneBank EMBL AJ784158 Gene Information Gene Name afpA NCBI Gene ID Protein Information Protein Name Antifreeze protein UniProtKB/SwissProt ID Q68VA9 NCBI RefSeq EMBL-CDS CAH04619.1 UniProtKB Sequence >tr|Q68VA9|Q68VA9_PSEPU Antifreeze protein OS=Pseudomonas putida GN=afpA PE=4 SV=1 MQYDSPITNTEFQTFLTTSSISDDTAAAISTLLNLDSADTINLASWDGVNAPEIPTGQEG AADVVIVNVPGAATDLVPVEIPDSLNSAKAFIFDSNASLAVTFDAPVAAESASLARVAAD TTAGIEFLVTTGAGNDVITVNGDQNSYIDAGNGNDTIVTGNGNNTVVAGAGNNNVTTGTG NDTIILSGTGHTDIVNTGAGYDVVQLDGSAADYTITAGNSNNVTLTGAQTAAITGAEFLT FADGSSVALAQSEAEASALRLYEGILGRDADQGGAQNFIAQVEAGTALTDIANSFLNSAE FGGAATEASIDSLYTSLLGRGADTAGSDSWEAIIANGGSLADVAAGIAGSAEAQEQDQSN GTFVDSLYLNALGRPSDEAGHDAWVAQLFNGASRAEVAAGIVGSAEAAEKINSDFIDALY LSATGRASDEAGKAGWTEVLANGGTQADVAIGIVGSQEAIAHNDNVVVLHGAV Sequence length 473 AA Subcellular Location Secreted Function This 164-kDa glycoprotein has both antifreez activity and ice-nucleation activity. Glycosylation Status Glycosylation Type Information currently not available with us. Technique(s) used for Glycosylation Detection Periodic acid-Schiff (PAS) staining Literatures Reference(s) 1) Xu, H., Griffith, M., Patten, C.L. and Glick, B.R. (1998) Isolation and characterization of an antifreeze protein with ice nucleation activity from the plant growth promoting rhizobacterium Pseudomonas putida GR12-2. Can J Microbiol, 44, 64–73. Year of Identification 1998