ProGlyProt ID BU228 Organism Information Organism Name Mycobacterium vaccae Domain Bacteria Classification Family: Mycobacteriaceae Suborder: Corynebacterineae Order: Actinomycetales Subclass: Actinobacteridae Class: Actinobacteria Division or phylum: "Actinobacteria" Taxonomic ID (NCBI) Genome Sequence (s) GeneBank EMBL Gene Information Gene Name NCBI Gene ID Protein Information Protein Name PS4A (water soluble protein- polysaccharide) UniProtKB/SwissProt ID NCBI RefSeq EMBL-CDS UniProtKB Sequence Sequence length Subcellular Location Intracellular Function PS4A significantly decreased tumor incidence in vivo, produced activation of murine peritoneal macrophages. However, the antineoplastic activity was only attributable to the high molecular weight fraction of the protein-polysaccharia complex. The low molecular weight fraction had no antineoplastic activity in viva despite stimulation of TNF-a production in vitro. In vitro experiments also demonstrated that although all PS4A components significantly increased TNF-α production by macrophages, the Glycosylation Status Glycosylation Type Technique(s) used for Glycosylation Detection Literatures Reference(s) 1) Tian, X.X., Li, A., Farrugia, I.V., Mo, X., Crich, D. and Groves, M.J. (2000) Isolation and identification of poly-alpha-(1-->4)-linked 3-O-methyl-D-mannopyranose from a hot-water extract of Mycobacterium vaccae. Carbohydr Res, 324, 38-44. [PubMed: 10723610] 2) Tian, X.X., Li, A., Zhou, W., Farrugia, I.V. and Groves, M.J. (1999) Isolation and biological activities of an antineoplastic protein-polysaccharide complex (PS4A) obtained from Mycobacterium vaccae. Anticancer Res, 19, 237-243. [PubMed: 10226548] Year of Identification 2000