ProGlyProt ID BU168 Organism Information Organism Name Geobacillus (formerly Bacillus) stearothermophilus Domain Bacteria Classification Family: Bacillaceae Order: Bacillales Class: Bacilli (or Firmibacteria) Division or phylum: "Firmicutes" Taxonomic ID (NCBI) 1422 Genome Sequence (s) GeneBank EMBL AF228338 Gene Information Gene Name sbsD NCBI Gene ID Protein Information Protein Name SbsD (S-layer glycoprotein) UniProtKB/SwissProt ID Q9KIQ5 NCBI RefSeq EMBL-CDS AAF34763.2 UniProtKB Sequence >tr|Q9KIQ5|Q9KIQ5_GEOSE SbsD OS=Geobacillus stearothermophilus GN=sbsD PE=4 SV=1 MDKKKAVKLATASAVAASAFVAANPHASQAATDVATVVSQAKAQMKEAYYTYSHTVTETG KLPNISDVYAAYNKAKQAYANAVAVVNKAGGAKKDAYLADLQATYETYVFKANPKSGEAR VATYIDAYNYATKLDAMRQELKAAVEAKDLKKAEELYHKISYELKTRTVILDRVYGQSTR ELLRSTFKADAQALRDSLIYDITVAMKAREAQDAAKAGNLDKAKAALDQVNQYVSKVTDA FKAELQKAAQDANAAYEAALPPKVESVTAVNAKTLEIKFNKAVDVATVIDNKGTSDTSDD VVKATAITLTAIDGQGSVSTVKASLSDDKKTLKLVADGSQFFTKRYVVDIKNVKTLDGKD VPSYTTTIDTTDSVRPSVLSSSYADNGLTLKVKFSEPLASVGTVKLYDGTTEISVSPKFT AGDDEMTINLASSSVPVNKDLTLKIFGAVDYNGNVINPNPAELTVKKTTVDTTKPTVQNI EAVNTKTVKVTFSEKLLSNPTIKIGGQTASVSVDSTGLVYTATLANALSEGVYAVEVSDY KDLAGNAGDTYTKVVQLKADTTAPKFVSSQVVKIDGVEHLVLTFDEEVTTGSNITVVQAS DKYVDENNVLKSVGTTLTTTSDNFKLYLPTDGKSKSVALNISSLPKGTYTVTLPDRLVSD LAGNPYAERKQITFVRGSDSLTTKPALDSSYDNNGVKADNNNELVFAFTQNLDASALNLS NFNINGLAVTKAVFDGDTKHIRVTLAPGANTWTGTHVITISNIKNTSGLVMDTVTVSEFM KENVAPTFTATLTSADVIRVDFSEPVANATISSPLSANNFTVKVDGNVVTVSNVYEDNNA TNQVQGSTGYKTVYLKLQSPVSDLSKPITLSATGIVDVDQTGAITNNNLVGNNVSNAVVN VAK Sequence length 903 AA Subcellular Location Surface Function Biosynthesis of S-layer glycoprotein occurs due to S-layer variation at higher temperature. Glycosylation Status Glycosylation Type O(Ser/Thr)- linked Technique(s) used for Glycosylation Detection Periodate-Schiff (PAS) reagent staining, deglycosylation with trifluoromethanesulfonic acid Literatures Reference(s) 1) Egelseer, E.M., Danhorn, T., Pleschberger, M., Hotzy, C., Sleytr, U.B. and Sara, M. (2001) Characterization of an S-layer glycoprotein produced in the course of S-layer variation of Bacillus stearothermophilus ATCC 12980 and sequencing and cloning of the sbsD gene encoding the protein moiety. Arch Microbiol, 177, 70-80. [PubMed: 11797047] Year of Identification 2001