ProGlyProt ID BU166 Organism Information Organism Name Fibrobacter (Bacteroides) succinogenes S85 Domain Bacteria Classification Family: "Fibrobacteraceae" Order: "Fibrobacterales" Class: "Fibrobacteria" Division or phylum: "Fibrobacteres" Taxonomic ID (NCBI) 59374 Genome Sequence (s) GeneBank CP001792.1 EMBL CP001792 Gene Information Gene Name Fisuc_2992 NCBI Gene ID 8523639 Protein Information Protein Name Cellobiosidase UniProtKB/SwissProt ID A7UG54 NCBI RefSeq YP_003251054.1 EMBL-CDS ACX76572.1 UniProtKB Sequence >tr|A7UG54|A7UG54_FIBSS Chloride-stimulated cellobiosidase OS=Fibrobacter succinogenes (strain ATCC 19169 / S85) GN=Fisuc_2992 PE=3 SV=1 MNFKKNKAFAIAATVAMALSVPSFAQLNNGDMEYGDGGWYLWNNPDGPAVAESQIAVQGL GVDGSQGAKVVVKELPNPWWGLQLQPPKFLADSGFYKLSFKAKGNMPINSVVQGGPPDYR QKESASFDLTDKWQTYEMIFLADQKGYGLNNITFQIGLKKGWIQLDDVEVEKMDEMSDPS WYNNADARIDSLRKVDFTVKANPGEKVHVKLLRHSFPFGTALALYDTKDSTENWYRNAAK KYFWHGVSENQFKWPEYEPKKGKIKRDEMKEYTDFTAQNHWKLRGHALMWSHQGYGFDKH YSNKGRCEEMAEKLKARIYRDLKEYKGKITEYDVWNEPIHESWTFNKCGWGILDSAFIWA HKADPSAFLYINDYNVVAAGETDRYYGLIKGMLERKVPVMGIGVQCHFGLRPVVPSLIKE RLDKLASLGLPIKVTEFDVGDWQAGMNDTEEVQAEKFETFLRTAFSHPAVNGIVFWGFWD NRHWVKNGGMIASDGREKPAAKRVYDLWHKVWTTDLYATADENGEAKFRGFKGYYQVNAG DKKFQITVK Sequence length 549 AA Subcellular Location Extracellular as well as periplasmic Function Extracellular cellobiosidase acitivity is stimulated by chloride. Cl-stimulated cellobiosidase has also been found to be associated with cell membrane debris. It is capable of hydrolysing p-nitrophenyl lactoside and p-nitrophenyl cellobioside. A detectable activity on carboxymethyl cellulose has also been found. Glycosylation Status Glycosylation Type Information currently not available with us. Technique(s) used for Glycosylation Detection Periodic acid-Schiff (PAS) staining Literatures Reference(s) 1) Huang, L., Forsberg, C.W. and Thomas, D.Y. (1988) Purification and characterization of a chloride-stimulated cellobiosidase from Bacteroides succinogenes S85. J Bacteriol, 170, 2923-2932. [PubMed: 3384799] Year of Identification 1988