ProGlyProt ID BU158 Organism Information Organism Name Enterococcus hirae (formerly Streptococcus faecium Domain Bacteria Classification Family: Enterococcaceae Order: Lactobacillales Class: Bacilli (or Firmibacteria) Division or phylum: "Firmicutes" Taxonomic ID (NCBI) 1354 Genome Sequence (s) GeneBank EMBL M77639 Gene Information Gene Name NCBI Gene ID Protein Information Protein Name N-acetylmuramoylhydrolase (Muramidase-2) UniProtKB/SwissProt ID P39046 NCBI RefSeq EMBL-CDS AAA24776.1 UniProtKB Sequence >sp|P39046|MUR2_ENTHR Muramidase-2 OS=Enterococcus hirae PE=1 SV=1 MENIARKERRRLNETKRFRKVKRSAALVGTAMVGCSVAAPLIQPVQVDADQTPTQFGARI NTAAFIAEIATYAQPIAQANDLYASVMIAQAVVESGWGSSALSQAPYYNLFGIKGSYQGQ TVYMDTLEYLNNKWVSKKEPFRQYPSFAESFNDNAYVLRNTSFGNGYYYAGTWKSNTKSY TDATACLTGRYATDPGYAGKLNNIITTYGLTKYDTPASGNAGGGVTIGNGGNTGNTSNSG STSGNSGGSATTTGTTYTVKSGDSVWGISHSFGITMAQLIEWNNIKNNFIYPGQKLTIKG GQSAGSSTTNTGNNASSGNTSGNTNTSGSTGQATGAKYTVKSGDSVWKIANDHGISMNQL IEWNNIKNNFVYPGQQLVVSKGSSSASGSTSNTSTGNTSSNTANTGSTTSGSTYTVKAGE SVWSVSNKFGISMNQLIQWNNIKNNFIYPGQKLIVKGGSSSSNASTSTANNKNTASSNTS STATGQATYTVKAGESVWGVANKNGISMNQLIEWNNIKNNFIYPGQKLIVKGGSSKASAT ATIKPTASTPASTTPTASSTGDTKYTVKAGESVWGVANKHHITMDQLIEWNNIKNNFIYP GQEVIVKKGTAQSTPAKSDEKTYTVKAGESVWGVADSHGITMNQLIEWNNIKNNFIYPGQ QLIVKK Sequence length 666 AA Subcellular Location Cell wall bound (Secreted) Function It has an endogenous, autolytic peptidoglycan hydrolase activity with a single specificity that is β-1,4-N-acetylmuramoylhydrolase (muramidase; EC 3.2.1.17). Glycosylation Status Glycosylation Type Information currently not available with us. Technique(s) used for Glycosylation Detection Periodic acid-Schiff (PAS) staining and binding to concanavalin A-Sepharose 4B column Literatures Reference(s) 1) Kawamura, T. and Shockman, G.D. (1983) Purification and some properties of the endogenous, autolytic N-acetylmuramoylhydrolase of Streptococcus faecium, a bacterial glycoenzyme. J Biol Chem, 258, 9514-9521. [PubMed: 6874701] Year of Identification 1983