ProGlyProt ID BU148 Organism Information Organism Name Clostridium thermocellum ATCC 27405 Domain Bacteria Classification Family: Clostridiaceae Order: Clostridiales Class: Clostridia Division or phylum: "Firmicutes" Taxonomic ID (NCBI) 203119 Genome Sequence (s) GeneBank CP000568.1 EMBL CP000568 Gene Information Gene Name cipA (Cthe_3077) NCBI Gene ID 4809951 Protein Information Protein Name Cellulolosome complex (Cellulosomal-scaffolding pr UniProtKB/SwissProt ID Q06851 NCBI RefSeq YP_001039466.1 EMBL-CDS ABN54273.1 UniProtKB Sequence >sp|Q06851|CIPA_CLOTH Cellulosomal-scaffolding protein A OS=Clostridium thermocellum (strain ATCC 27405 / DSM 1237) GN=cipA PE=1 SV=2 MRKVISMLLVVAMLTTIFAAMIPQTVSAATMTVEIGKVTAAVGSKVEIPITLKGVPSKGM ANCDFVLGYDPNVLEVTEVKPGSIIKDPDPSKSFDSAIYPDRKMIVFLFAEDSGRGTYAI TQDGVFATIVATVKSAAAAPITLLEVGAFADNDLVEISTTFVAGGVNLGSSVPTTQPNVP SDGVVVEIGKVTGSVGTTVEIPVYFRGVPSKGIANCDFVFRYDPNVLEIIGIDPGDIIVD PNPTKSFDTAIYPDRKIIVFLFAEDSGTGAYAITKDGVFAKIRATVKSSAPGYITFDEVG GFADNDLVEQKVSFIDGGVNVGNATPTKGATPTNTATPTKSATATPTRPSVPTNTPTNTP ANTPVSGNLKVEFYNSNPSDTTNSINPQFKVTNTGSSAIDLSKLTLRYYYTVDGQKDQTF WCDHAAIIGSNGSYNGITSNVKGTFVKMSSSTNNADTYLEISFTGGTLEPGAHVQIQGRF AKNDWSNYTQSNDYSFKSASQFVEWDQVTAYLNGVLVWGKEPGGSVVPSTQPVTTPPATT KPPATTKPPATTIPPSDDPNAIKIKVDTVNAKPGDTVNIPVRFSGIPSKGIANCDFVYSY DPNVLEIIEIKPGELIVDPNPDKSFDTAVYPDRKIIVFLFAEDSGTGAYAITKDGVFATI VAKVKSGAPNGLSVIKFVEVGGFANNDLVEQRTQFFDGGVNVGDTTVPTTPTTPVTTPTD DSNAVRIKVDTVNAKPGDTVRIPVRFSGIPSKGIANCDFVYSYDPNVLEIIEIEPGDIIV DPNPDKSFDTAVYPDRKIIVFLFAEDSGTGAYAITKDGVFATIVAKVKSGAPNGLSVIKF VEVGGFANNDLVEQKTQFFDGGVNVGDTTEPATPTTPVTTPTTTDDLDAVRIKVDTVNAK PGDTVRIPVRFSGIPSKGIANCDFVYSYDPNVLEIIEIEPGDIIVDPNPDKSFDTAVYPD RKIIVFLFAEDSGTGAYAITKDGVFATIVAKVKSGAPNGLSVIKFVEVGGFANNDLVEQK TQFFDGGVNVGDTTEPATPTTPVTTPTTTDDLDAVRIKVDTVNAKPGDTVRIPVRFSGIP SKGIANCDFVYSYDPNVLEIIEIEPGDIIVDPNPDKSFDTAVYPDRKIIVFLFAEDSGTG AYAITKDGVFATIVAKVKEGAPNGLSVIKFVEVGGFANNDLVEQKTQFFDGGVNVGDTTE PATPTTPVTTPTTTDDLDAVRIKVDTVNAKPGDTVRIPVRFSGIPSKGIANCDFVYSYDP NVLEIIEIEPGELIVDPNPTKSFDTAVYPDRKMIVFLFAEDSGTGAYAITEDGVFATIVA KVKSGAPNGLSVIKFVEVGGFANNDLVEQKTQFFDGGVNVGDTTEPATPTTPVTTPTTTD DLDAVRIKVDTVNAKPGDTVRIPVRFSGIPSKGIANCDFVYSYDPNVLEIIEIEPGDIIV DPNPDKSFDTAVYPDRKIIVFLFAEDSGTGAYAITKDGVFATIVAKVKEGAPNGLSVIKF VEVGGFANNDLVEQKTQFFDGGVNVGDTTVPTTSPTTTPPEPTITPNKLTLKIGRAEGRP GDTVEIPVNLYGVPQKGIASGDFVVSYDPNVLEIIEIEPGELIVDPNPTKSFDTAVYPDR KMIVFLFAEDSGTGAYAITEDGVFATIVAKVKEGAPEGFSAIEISEFGAFADNDLVEVET DLINGGVLVTNKPVIEGYKVSGYILPDFSFDATVAPLVKAGFKVEIVGTELYAVTDANGY FEITGVPANASGYTLKISRATYLDRVIANVVVTGDTSVSTSQAPIMMWVGDIVKDNSINL LDVAEVIRCFNATKGSANYVEELDINRNGAINMQDIMIVHKHFGATSSDYDAQ Sequence length 1853 AA Subcellular Location Secreted (Cell surface) Function Cellulose-binding, multicellulase-containing protein complex, termed the cellulosome, which displays endo-glucanase and glucohydrolase activity and mediates strong adherence of the bacterium to the insoluble substrate. Also called cellulose-interacting protein A. Glycosylation Status Glycosylation Type O(Thr)-linked Technique(s) used for Glycosylation Detection Literatures Reference(s) 1) Bayer EA, Shoham Y, Lamed R (2000) The Cellulosome. An Extracellular Organelle for Degrading Plant Cell Wall Polysaccharides. In: Doyle RJ (ed) Glycomicrobiology. Kluwer Academic/Plenum Publishers, New York, p 387 2) Shoham, Y., Lamed, R. and Bayer, E.A. (1999) The cellulosome concept as an efficient microbial strategy for the degradation of insoluble polysaccharides. Trends Microbiol, 7, 275-281. [PubMed: 10390637] 3) Gerwig, G.J., Kamerling, J.P., Vliegenthart, J.F., Morag, E., Lamed, R. and Bayer, E.A. (1993) The nature of the carbohydrate-peptide linkage region in glycoproteins from the cellulosomes of Clostridium thermocellum and Bacteroides cellulosolvens. J Biol Chem, 268, 26956-26960. [PubMed: 8262930] 4) Gerwig, G.J., Kamerling, J.P., Vliegenthart, J.F., Morag, E., Lamed, R. and Bayer, E.A. (1991) Primary structure of O-linked carbohydrate chains in the cellulosome of different Clostridium thermocellum strains. Eur J Biochem, 196, 115-122. [PubMed: 2001693] Year of Identification 1989