ProGlyProt ID BU145 Organism Information Organism Name Clostridium difficile C253 Domain Bacteria Classification Family: Clostridiaceae Order: Clostridiales Class: Clostridia Division or phylum: "Firmicutes" Taxonomic ID (NCBI) Genome Sequence (s) GeneBank EMBL Gene Information Gene Name NCBI Gene ID Protein Information Protein Name Surface layer glycoproteins UniProtKB/SwissProt ID NCBI RefSeq EMBL-CDS UniProtKB Sequence Sequence length Subcellular Location Surface Function Two glycoproteins P36 and P47 are the components of the S-layer. Glycosylation Status Glycosylation Type Information currently not available with us. Technique(s) used for Glycosylation Detection DIG glycan/protein double labeling kit and thymol staining method. Literatures Reference(s) 1) Calabi, E., Ward, S., Wren, B., Paxton, T., Panico, M., Morris, H., Dell, A., Dougan, G. and Fairweather, N. (2001) Molecular characterization of the surface layer proteins from Clostridium difficile. Mol Microbiol, 40, 1187-1199. [PubMed: 11401722] 2) Karjalainen, T., Waligora-Dupriet, A.J., Cerquetti, M., Spigaglia, P., Maggioni, A., Mauri, P. and Mastrantonio, P. (2001) Molecular and genomic analysis of genes encoding surface-anchored proteins from Clostridium difficile. Infect Immun, 69, 3442-3446. [PubMed: 11292772] 3) Cerquetti, M., Molinari, A., Sebastianelli, A., Diociaiuti, M., Petruzzelli, R., Capo, C. and Mastrantonio, P. (2000) Characterization of surface layer proteins from different Clostridium difficile clinical isolates. Microb Pathog, 28, 363-372. [PubMed: 10839973] 4) Mauri, P.L., Pietta, P.G., Maggioni, A., Cerquetti, M., Sebastianelli, A. and Mastrantonio, P. (1999) Characterization of surface layer proteins from Clostridium difficile by liquid ch Year of Identification 1999