ProGlyProt ID BU125 Organism Information Organism Name Bacteroides fragilis Domain Bacteria Classification Family: Bacteroidaceae Order: "Bacteroidales" Class: "Bacteroidia" Division or phylum: "Bacteroidetes" Taxonomic ID (NCBI) 272559 Genome Sequence (s) GeneBank CR626927.1 EMBL CR626927 Gene Information Gene Name pepO (BF3918) NCBI Gene ID 3286398 Protein Information Protein Name BF3918 UniProtKB/SwissProt ID Q5L8J2 NCBI RefSeq YP_213499.1 EMBL-CDS CAH09595.1 UniProtKB Sequence >tr|Q5L8J2|Q5L8J2_BACFN Putative endopeptidase OS=Bacteroides fragilis (strain ATCC 25285 / NCTC 9343) GN=pepO PE=4 SV=1 MKVIKYLPILAICCMATGCNSKKEAVLTSGIDLANLDTTALPGTSFYQYACGGWMKNHPL TDEYSRFGSFDMLAENNRQQLRGLIEGLAAEKHEAGSIAQKVGELYNIAMDSVKLNKEGA APIKPELEKIGAIKDKAEIYPLIVEMQKRGMYPYFILYVSADDMNSNENMVHTMQGGLGM GERDYYLEDDAQTKEIRDKYQQHVSKMFQLAGYDEATARKAVKAVMNIETRLAQSARSQV ELRDPHANYNKKTLEELQKEYPSFAWDVFFSTAGLNNLKEVNIGQPDALKEVNAIIDTVS LEDQILYLQWNLINSAANYLSDDFIAQDFDFYGRTMSGKKEMQPRWKRAVSTVDGSLGEA VGQMYVEKYFPAAAKERMVALVKNLQESLGERIKGLSWMGEETKEKALEKLATFHVKIGY PDKWKDYSSLEIKDDSYWANIERANQWSYNEMIGKYGKPVDKDEWYMTPQTVNAYYNPTT NEICFPAGILQYPFFDMNADDAFNYGAIGVVIGHEMTHGFDDQGRQYDKDGNLKDWWTAE DAKNFEARAAVMANFFDSIEVAPGVHANGEFTLGENIADHGGLQVSYQAFKKATAAAPLK IENGFTPEQRFFLSYANVWAGNIRPEEILKRTKTDPHSLGKWRVDGALPQIGAWYEAFNI TEKDPMYLPVDKRVSIW Sequence length 677 AA Subcellular Location Outer membrane - facing periplasm Function Zinc endopeptidase (M13 family). Glycosylation Status Glycosylation Type Information currently not available with us. Technique(s) used for Glycosylation Detection Mass shift detected on SDS-polyacrylamide gel, AAL (Aleuria aurantia lectin) reactivity, Pro-Q Emerald Glycostaining and reactivity towards anti-glycan antiserum Literatures Reference(s) 1) Fletcher, C.M., Coyne, M.J., Villa, O.F., Chatzidaki-Livanis, M. and Comstock, L.E. (2009) A general O-glycosylation system important to the physiology of a major human intestinal symbiont. Cell, 137, 321-331. [PubMed: 19379697] Year of Identification 2009