ProGlyProt ID BU112 Organism Information Organism Name Bacillus circulans K-1 Domain Bacteria Classification Family: Bacillaceae Order: Bacillales Class: Bacilli (or Firmibacteria) Division or phylum: "Firmicutes" Taxonomic ID (NCBI) 1397 Genome Sequence (s) GeneBank EMBL AB007123 Gene Information Gene Name G-gene NCBI Gene ID Protein Information Protein Name β-1,4-mannanase (G-enzyme) UniProtKB/SwissProt ID O66185 NCBI RefSeq EMBL-CDS BAA25878.1 UniProtKB Sequence >tr|O66185|O66185_BACCI Mannanase OS=Bacillus circulans PE=4 SV=1 MGWFLVILRKWLIAFVAFLLMFSWTGQLTNKAHAASGFYVSGTKLLDATGQPFVMRGVNH AHTWYKDQLSTAIPAIAKTGANTIRIVLANGHKWTLDDVNTVNNILTLCEQNKLIAVLEV HDATGSDSLSDLDNAVNYWIGIKSALIGKEDRVIINIANEWYGTWDGVAWANGYKQAIPK LRNAGLTHTLIVDSAGWGQYPDSVKNYGTEVLNADPLKNTVFSIHMYEYAGGNASTVKSN IDGVLNKNLALIIGEFGGQHTNGDVDEATIMSYSQEKGVGWLAWSWKGNSSDLAYLDMTN DWAGNSLTSFGNTVVNGSNGIKATSVLSGIFGGVTPTSSPTSTPTSTPTSTPTPTPSPTP SPGNNGTILYDFETGTQGWSGNNISGGPWVTNEWKATGAQTLKADVSLQSNSTHSLYITS NQNLSGKSSLKATVKHANWGNIGNGIYAKLYVKTGSGWTWYDSGENLIQSNDGTILTLSL SGISNLSSVKEIGVEFRASSNSSGQSAIYVDSVSLQ Sequence length 516 AA Subcellular Location Intracellular Function Degrades guar gum which consists of a galactomannosaccharide from the endosperm of guar seeds. Glycosylation Status Glycosylation Type Information currently not available with us. Technique(s) used for Glycosylation Detection Mass shift detected on SDS-polyacrylamide gel, periodic acid-Schiff (PAS) staining Literatures Reference(s) 1) Yoshida, S., Sako, Y. and Uchida, A. (1998) Cloning, sequence analysis, and expression in Escherichia coli of a gene coding for an enzyme from Bacillus circulans K-1 that degrades guar gum. Biosci Biotechnol Biochem, 62, 514-520. [PubMed: 9571781] Year of Identification 1998