ProGlyProt ID AU107 Organism Information Organism Name Haloferax volcanii DS2 Domain Archaea Classification Family: Halobacteriaceae Order: Halobacteriales Class: Halobacteria or Halomebacteria Division or phylum: "Euryarchaeota" Taxonomic ID (NCBI) 309800 Genome Sequence (s) GeneBank CP001953.1 EMBL CP001953 Gene Information Gene Name lccA NCBI Gene ID 8919198 Protein Information Protein Name LccA, an Archaeal Laccase UniProtKB/SwissProt ID D4GPK6 NCBI RefSeq YP_003533285.1 EMBL-CDS ADE01515.1 UniProtKB Sequence >tr|D4GPK6|D4GPK6_HALVD LccA multicopper oxidase (MCO) OS=Haloferax volcanii (strain ATCC 29605 / DSM 3757 / IFO 14742 / NCIMB 2012 / DS2) GN=lccA PE=4 SV=1 MTDWSRRRFLQTGAALGIAGTLPQTTTEVSAASPTLEKFVQPLPIPSVREPDGQRDGADA YEIAVTEFTQQLHPDLPETTVWGFDGSYPGPTIEADAGSPVHVRFDNSGLPSEHLFPVDD RLGGTTAENHPGYDGPVPEVRTVTHFHGLELDPANDGQSDMWTSPGGVEGPRFDSAWQEL PMEQGRTTSTYHDHTLGITRLNAYAGLLGLYSITTDAERELGLPSGDYDIPLLLQDKEFN DDGSLHYPEEFVSAFLGDTAVVNGAVWPYVEVEPRRYRFRILNGANHRSFDLQLESESGS GVPTMYQFAPGHGFLESVVPIGPNGDLDSLLLTPFERGELVVDFSDHAGETLTLANGADM GPELTDLVEFRVSDPSTPPEDASADPTSLSLPTPASYDESDARVTREMTLGTEVRNGLIT HTLNGHVFGDEDAPVYPQLGATEIWELQNESGGRHPIHLHLVTFRVIGRGPDGTQPPDPN ELGPKDTVRVDPGERVRILVTFEGYTGQFPWHCHMLEHEDNKMMIPFVVENPVADYANEE NVVDATGLTDAVGDWRNETLETEVLLEVIDQWRSGDEVA Sequence length 579 AA Subcellular Location Secreted Function Belongs to the family of Multicopper oxidases (MCOs). Laccases couple the oxidation of phenolic compounds to the reduction of O2. Glycosylation Status Glycosylation Type Information currently not available with us. Technique(s) used for Glycosylation Detection Carbohydrate content determined by phenol-sulfuric acid assay, Pro-Q Emerald 300 staining and mass shift (of 10-20 kDa) detected after deglycosylation with TFMS (trifluoromethanesulfonic acid). Literatures Reference(s) 1) Uthandi, S., Saad, B., Humbard, M.A. and Maupin-Furlow, J.A. (2010) LccA, an archaeal laccase secreted as a highly stable glycoprotein into the extracellular medium by Haloferax volcanii. Appl Environ Microbiol, 76, 733-743. [PubMed: 19966030] Year of Identification 2010