ProGlyProt ID BC170 Organism Information Organism Name Pseudomonas aeruginosa PAO1 Domain Bacteria Classification Family: Pseudomonadaceae Order: Pseudomonadales Class: Gammaproteobacteria Division or phylum: "Proteobacteria" Taxonomic ID (NCBI) 287 Genome Sequence (s) GeneBank AE004091.2 EMBL AE004091 Gene Information Gene Name fliC (PA1092) NCBI Gene ID 882052 GenBank Gene Sequence 882052 Protein Information Protein Name Type b Flagellin UniProtKB/SwissProt ID P72151 NCBI RefSeq NP_249783.1 EMBL-CDS AAG04481.1 UniProtKB Sequence >sp|P72151|FLICB_PSEAE B-type flagellin OS=Pseudomonas aeruginosa GN=fliC PE=1 SV=2 MALTVNTNIASLNTQRNLNASSNDLNTSLQRLTTGYRINSAKDDAAGLQISNRLSNQISG LNVATRNANDGISLAQTAEGALQQSTNILQRIRDLALQSANGSNSDADRAALQKEVAAQQ AELTRISDTTTFGGRKLLDGSFGTTSFQVGSNAYETIDISLQNASASAIGSYQVGSNGAG TVASVAGTATASGIASGTVNLVGGGQVKNIAIAAGDSAKAIAEKMDGAIPNLSARARTVF TADVSGVTGGSLNFDVTVGSNTVSLAGVTSTQDLADQLNSNSSKLGITASINDKGVLTIT SATGENVKFGAQTGTATAGQVAVKVQGSDGKFEAAAKNVVAAGTAATTTIVTGYVQLNSP TAYSVSGTGTQASQVFGNASAAQKSSVASVDISTADGAQNAIAVVDNALAAIDAQRADLG AVQNRFKNTIDNLTNISENATNARSRIKDTDFAAETAALSKNQVLQQAGTAILAQANQLP QAVLSLLR Sequence length 488 AA Subcellular Location Secreted Function Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella. It has been shown to have a role in the stimulation of inflammatory response via Toll-like receptor 5. Protein Structure PDB ID Glycosylation Status Glycosylation Type O (Ser) linked Experimentally Validated Glycosite(s) in Full Length Protein (Initiator Met removed) S192, S196 Experimentally Validated Glycosite(s ) in Mature Protein S191, S195 Glycosite(s) Annotated Protein Sequence >sp|P72151|FLICB_PSEAE B-type flagellin OS=Pseudomonas aeruginosa GN=fliC PE=1 SV=2 MALTVNTNIASLNTQRNLNASSNDLNTSLQRLTTGYRINSAKDDAAGLQISNRLSNQISG LNVATRNANDGISLAQTAEGALQQSTNILQRIRDLALQSANGSNSDADRAALQKEVAAQQ AELTRISDTTTFGGRKLLDGSFGTTSFQVGSNAYETIDISLQNASASAIGSYQVGSNGAG TVASVAGTATAS*(192)GIAS*(196)GTVNLVGGGQVKNIAIAAGDSAKAIAEKMDGAIPNLSARARTVF TADVSGVTGGSLNFDVTVGSNTVSLAGVTSTQDLADQLNSNSSKLGITASINDKGVLTIT SATGENVKFGAQTGTATAGQVAVKVQGSDGKFEAAAKNVVAAGTAATTTIVTGYVQLNSP TAYSVSGTGTQASQVFGNASAAQKSSVASVDISTADGAQNAIAVVDNALAAIDAQRADLG AVQNRFKNTIDNLTNISENATNARSRIKDTDFAAETAALSKNQVLQQAGTAILAQANQLP QAVLSLLR Sequence Around Glycosites (21 AA) VASVAGTATASGIASGTVNLV AGTATASGIASGTVNLVGGGQ Glycosite Sequence Logo seqlogo Glycosite Sequence Logo Technique(s) used for Glycosylation Detection NanoES-MS (nanoelectrospray mass spectrometry) for intact mass analysis Technique(s) used for Glycosylated Residue(s) Detection LC-ES-MS/MS (liquid chromatography electrospray tandem mass spectrometry) analysis analysis of chymotryptic or tryptic digests of the protein after β-elimination with ammonium hydroxide. Protein Glycosylation- Implication Glycan Information Glycan Annotation Linkage: Deoxyhexose-Ser. (356 Da) A single deoxyhexose monosaccharide linked to a unique modification of mass 209 Da which contains a phosphate moiety. Technique(s) used for Glycan Identification Intact mass analysis of flagellin by nanoelectrospray MS and LC-nES-MS/MS (liquid chromatography-nanoelectrospray tandem mass spectrometry) analysis of chymotryptic or tryptic digests of the protein. Protein Glycosylation linked (PGL) gene(s) OST Gene Name pilO (tfpO) OST NCBI Gene ID 880985 OST GenBank Gene Sequence 880985 OST Protein Name PilO (TfpO) OST UniProtKB/ SwissProt ID Q51353 OST NCBI RefSeq NP_253729.1 OST EMBL-CDS AAG08427.1 OST UniProtKB Sequence >tr|Q51353|Q51353_PSEAE PilO protein OS=Pseudomonas aeruginosa GN=pilO PE=1 SV=1 MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQ QAAEEETLKQQFSTKAFQAANLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTG LGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATFVSGVSSLPRIVTLHDFEIKP VAPGSTSKLRMSILAKTYRYNDKGLKK OST EC Number (BRENDA) 2.4.1.119 OST Genome Context Q51353 Characterized Accessory Gene(s) FgtA (PA1091) is the glycosyltransferase that adds the deoxyhexose to the protein. PA1088 enzyme attaches the 209-Da modification to the deoxyhexose. PA1088, PA1089, PA1090, PA1091 constitute the flagellin glycosylation island. PGL Additional Links CAZy Literatures Reference(s) 1) Verma, A., Schirm, M., Arora, S.K., Thibault, P., Logan, S.M. and Ramphal, R. (2006) Glycosylation of b-Type flagellin of Pseudomonas aeruginosa: structural and genetic basis. J Bacteriol, 188, 4395-4403. [PubMed: 16740946] Additional Comments Year of Identification 2006 Year of Validation 2006