| ProGlyProt ID | BC170 |
| Organism Information |
| Organism Name | Pseudomonas aeruginosa PAO1 |
| Domain | Bacteria |
| Classification | Family: Pseudomonadaceae
Order: Pseudomonadales
Class: Gammaproteobacteria
Division or phylum: "Proteobacteria" |
| Taxonomic ID (NCBI) | 287 |
| Genome Sequence (s) |
| GeneBank | AE004091.2 |
| EMBL | AE004091 |
| Gene Information |
| Gene Name | fliC (PA1092) |
| NCBI Gene ID | 882052 |
| GenBank Gene Sequence | 882052 |
| Protein Information |
| Protein Name | Type b Flagellin |
| UniProtKB/SwissProt ID | P72151 |
| NCBI RefSeq | NP_249783.1 |
| EMBL-CDS | AAG04481.1 |
| UniProtKB Sequence | >sp|P72151|FLICB_PSEAE B-type flagellin OS=Pseudomonas aeruginosa GN=fliC PE=1 SV=2
MALTVNTNIASLNTQRNLNASSNDLNTSLQRLTTGYRINSAKDDAAGLQISNRLSNQISG
LNVATRNANDGISLAQTAEGALQQSTNILQRIRDLALQSANGSNSDADRAALQKEVAAQQ
AELTRISDTTTFGGRKLLDGSFGTTSFQVGSNAYETIDISLQNASASAIGSYQVGSNGAG
TVASVAGTATASGIASGTVNLVGGGQVKNIAIAAGDSAKAIAEKMDGAIPNLSARARTVF
TADVSGVTGGSLNFDVTVGSNTVSLAGVTSTQDLADQLNSNSSKLGITASINDKGVLTIT
SATGENVKFGAQTGTATAGQVAVKVQGSDGKFEAAAKNVVAAGTAATTTIVTGYVQLNSP
TAYSVSGTGTQASQVFGNASAAQKSSVASVDISTADGAQNAIAVVDNALAAIDAQRADLG
AVQNRFKNTIDNLTNISENATNARSRIKDTDFAAETAALSKNQVLQQAGTAILAQANQLP
QAVLSLLR
|
| Sequence length | 488 AA |
| Subcellular Location | Secreted |
| Function | Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella. It has been shown to have a role in the stimulation of inflammatory response via Toll-like receptor 5. |
| Protein Structure |
| PDB ID | |
| Glycosylation Status |
| Glycosylation Type | O (Ser) linked |
| Experimentally Validated Glycosite(s) in Full Length Protein | (Initiator Met removed) S192, S196 |
| Experimentally Validated Glycosite(s ) in Mature Protein | S191, S195
|
| Glycosite(s) Annotated Protein Sequence | >sp|P72151|FLICB_PSEAE B-type flagellin OS=Pseudomonas aeruginosa GN=fliC PE=1 SV=2
MALTVNTNIASLNTQRNLNASSNDLNTSLQRLTTGYRINSAKDDAAGLQISNRLSNQISG
LNVATRNANDGISLAQTAEGALQQSTNILQRIRDLALQSANGSNSDADRAALQKEVAAQQ
AELTRISDTTTFGGRKLLDGSFGTTSFQVGSNAYETIDISLQNASASAIGSYQVGSNGAG
TVASVAGTATAS*(192)GIAS*(196)GTVNLVGGGQVKNIAIAAGDSAKAIAEKMDGAIPNLSARARTVF
TADVSGVTGGSLNFDVTVGSNTVSLAGVTSTQDLADQLNSNSSKLGITASINDKGVLTIT
SATGENVKFGAQTGTATAGQVAVKVQGSDGKFEAAAKNVVAAGTAATTTIVTGYVQLNSP
TAYSVSGTGTQASQVFGNASAAQKSSVASVDISTADGAQNAIAVVDNALAAIDAQRADLG
AVQNRFKNTIDNLTNISENATNARSRIKDTDFAAETAALSKNQVLQQAGTAILAQANQLP
QAVLSLLR
|
| Sequence Around Glycosites (21 AA) | VASVAGTATASGIASGTVNLV
AGTATASGIASGTVNLVGGGQ
|
| Glycosite Sequence Logo | seqlogo |
| Glycosite Sequence Logo |  |
| Technique(s) used for Glycosylation Detection | NanoES-MS (nanoelectrospray mass spectrometry) for intact mass analysis |
| Technique(s) used for Glycosylated Residue(s) Detection | LC-ES-MS/MS (liquid chromatography electrospray tandem mass spectrometry) analysis analysis of chymotryptic or tryptic digests of the protein after β-elimination with ammonium hydroxide. |
| Protein Glycosylation- Implication | |
| Glycan Information |
| Glycan Annotation | Linkage: Deoxyhexose-Ser.
(356 Da) A single deoxyhexose monosaccharide linked to a unique modification of mass 209 Da which contains a phosphate moiety. |
| Technique(s) used for Glycan Identification | Intact mass analysis of flagellin by nanoelectrospray MS and LC-nES-MS/MS (liquid chromatography-nanoelectrospray tandem mass spectrometry) analysis of chymotryptic or tryptic digests of the protein. |
| Protein Glycosylation linked (PGL) gene(s) |
| OST Gene Name | pilO (tfpO) |
| OST NCBI Gene ID | 880985 |
| OST GenBank Gene Sequence | 880985 |
| OST Protein Name | PilO (TfpO) |
| OST UniProtKB/ SwissProt ID | Q51353 |
| OST NCBI RefSeq | NP_253729.1 |
| OST EMBL-CDS | AAG08427.1 |
| OST UniProtKB Sequence | >tr|Q51353|Q51353_PSEAE PilO protein OS=Pseudomonas aeruginosa GN=pilO PE=1 SV=1
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQ
QAAEEETLKQQFSTKAFQAANLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTG
LGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATFVSGVSSLPRIVTLHDFEIKP
VAPGSTSKLRMSILAKTYRYNDKGLKK
|
| OST EC Number (BRENDA) | 2.4.1.119 |
| OST Genome Context | Q51353 |
| Characterized Accessory Gene(s) | FgtA (PA1091) is the glycosyltransferase that adds the deoxyhexose to the protein. PA1088 enzyme attaches the 209-Da modification to the deoxyhexose. PA1088, PA1089, PA1090, PA1091 constitute the flagellin glycosylation island. |
| PGL Additional Links | CAZy |
| Literatures |
| Reference(s) | 1) Verma, A., Schirm, M., Arora, S.K., Thibault, P., Logan, S.M. and Ramphal, R. (2006) Glycosylation of b-Type flagellin of Pseudomonas aeruginosa: structural and genetic basis. J Bacteriol, 188, 4395-4403. [PubMed: 16740946] |
| Additional Comments | |
| Year of Identification | 2006 |
| Year of Validation | 2006 |
