| ProGlyProt ID | BC158 |
| Organism Information |
| Organism Name | Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) |
| Domain | Bacteria |
| Classification | Family: Neisseriaceae
Order: Neisseriales
Class: Betaproteobacteria
Division or phylum: "Proteobacteria" |
| Taxonomic ID (NCBI) | 242231 |
| Genome Sequence (s) |
| GeneBank | AE004969.1 |
| EMBL | AE004969 |
| Gene Information |
| Gene Name | NGO1043 |
| NCBI Gene ID | 3282593 |
| GenBank Gene Sequence | 3282593 |
| Protein Information |
| Protein Name | Hypothetical protein |
| UniProtKB/SwissProt ID | Q5F7X2 |
| NCBI RefSeq | YP_208127.1 |
| EMBL-CDS | AAW89715.1 |
| UniProtKB Sequence | >tr|Q5F7X2|Q5F7X2_NEIG1 Putative uncharacterized protein OS=Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) GN=NGO1043 PE=4 SV=1
MKKLLIAAMMAAALAACSQEAKQEVKEAAQAVESDVKDTAASAAESAASAVEEAKGQVKD
AAADAKASAEEAVTEAKDAAAETKEAVSEAAKDTLNKAADAAQEAADKMKDAAK
|
| Sequence length | 114 AA |
| Subcellular Location | |
| Function | |
| Protein Structure |
| PDB ID | |
| Glycosylation Status |
| Glycosylation Type | O (Ser) linked |
| Experimentally Validated Glycosite(s) in Full Length Protein | S88 |
| Experimentally Validated Glycosite(s ) in Mature Protein | S88 |
| Glycosite(s) Annotated Protein Sequence | >tr|Q5F7X2|Q5F7X2_NEIG1 Putative uncharacterized protein OS=Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) GN=NGO1043 PE=4 SV=1
MKKLLIAAMMAAALAACSQEAKQEVKEAAQAVESDVKDTAASAAESAASAVEEAKGQVKD
AAADAKASAEEAVTEAKDAAAETKEAVS*(88)EAAKDTLNKAADAAQEAADKMKDAAK
|
| Sequence Around Glycosites (21 AA) | DAAAETKEAVSEAAKDTLNKA |
| Glycosite Sequence Logo | seqlogo |
| Glycosite Sequence Logo |  |
| Technique(s) used for Glycosylation Detection | Immunoblotting with antiglycan antibodies |
| Technique(s) used for Glycosylated Residue(s) Detection | The sole hydroxyl-bearing residue in the glycopeptide |
| Protein Glycosylation- Implication | |
| Glycan Information |
| Glycan Annotation | O-AcHexDATDH (433 Da) and DATDH (229 Da) glycans. DATDH is 2,4 diacetamido-2,4,6 trideoxyhexose. |
| Technique(s) used for Glycan Identification | LC-ESI-MS/MS with CID (liquid chromatography-electrospray ionization–tandem mass spectrometry with collision-induced dissociation) |
| Protein Glycosylation linked (PGL) gene(s) |
| OST Gene Name | NGO0085 |
| OST NCBI Gene ID | 3282309 |
| OST GenBank Gene Sequence | 3282309 |
| OST Protein Name | PglB |
| OST UniProtKB/ SwissProt ID | Q5FAE1 |
| OST NCBI RefSeq | YP_207258.1 |
| OST EMBL-CDS | AAW88846.1 |
| OST UniProtKB Sequence | >tr|Q5FAE1|Q5FAE1_NEIG1 Pilin glycosylation protein OS=Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) GN=NGO0085 PE=4 SV=1
MSKAVKRLFDIIASASGLIVLSPVFLVLIYLIRKNLGSPVFFIRERPGKDGKPFKMVKFR
SMRDALDSDGIPLPDSERLTDFGKKLRATSLDELPELWNVLKGEMSLVGPRPLLMQYLPL
YNKFQNRRHEMKPGITGWAQVNGRNALSWDEKFSCDVWYTDNFSFWLDMKILFLTVKKVL
IKEGISAQGEATMPPFAGNRKLAVIGAGGHGKVVAELAAALGTYGEIVFLDDRTQGSVNG
FPVIGTTLLLENSLSPEQFDITVAVGNNRIRRQITENAAALGFKLPVLIHPDATVSPSAI
IGQGSVVMAKAVVQAGSVLKDGVIVNTAATVDHDCLLDAFVHISPGAHLSGNTRIGEESR
IGTGACSRQQTTVGSGVTAGAGAVIVCDIPDGMTVAGNPAKPLTGKNPKTGTA
|
| OST EC Number (BRENDA) | |
| OST Genome Context | Q5FAE1 |
| Characterized Accessory Gene(s) | |
| PGL Additional Links | CAZy |
| Literatures |
| Reference(s) | 1) Vik, A., Aas, F.E., Anonsen, J.H., Bilsborough, S., Schneider, A., Egge-Jacobsen, W. and Koomey, M. (2009) Broad spectrum O-linked protein glycosylation in the human pathogen Neisseria gonorrhoeae. Proc Natl Acad Sci U S A, 106, 4447-4452. [PubMed: 19251655] |
| Additional Comments | Sequone feature: S/T plus low complexity region. |
| Year of Identification | 2009 |
| Year of Validation | 2009 |
