ProGlyProt ID BC157 Organism Information Organism Name Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) Domain Bacteria Classification Family: Neisseriaceae Order: Neisseriales Class: Betaproteobacteria Division or phylum: "Proteobacteria" Taxonomic ID (NCBI) 242231 Genome Sequence (s) GeneBank AE004969.1 EMBL AE004969 Gene Information Gene Name NGO1328 NCBI Gene ID 3281927 GenBank Gene Sequence 3281927 Protein Information Protein Name CycB UniProtKB/SwissProt ID Q5F759 NCBI RefSeq YP_208390.1 EMBL-CDS AAW89978.1 UniProtKB Sequence >tr|Q5F759|Q5F759_NEIG1 Putative cytochrome OS=Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) GN=NGO1328 PE=4 SV=1 MKQLRDNKAQGSALFTLVSGIVIVIAVLYFLIKLAGSGSFGDVDATTEAATQTRIQPVGQ LTMGDGIPVGERQGEQIFGKICIQCHAADSNVPNAPKLEHNGDWAPRIAQGFDTLFQHAL NGFNAMPAKGGAADLTDQELKRAITYMANKSGGSFPNPDEAAPADNAASGTASAPADSAA PAEAKAEDKGAAAPAVGVDGKKVFEATCQVCHGGSIPGIPGIGKKDDWAPRIKKGKETLH KHALEGFNAMPAKGGNAGLSDDEVKAAVDYMANQSGAKF Sequence length 279 AA Subcellular Location Periplasm Function Cytochrome c5 Protein Structure PDB ID Glycosylation Status Glycosylation Type O (Ser) linked Experimentally Validated Glycosite(s) in Full Length Protein S173 Experimentally Validated Glycosite(s ) in Mature Protein S173 Glycosite(s) Annotated Protein Sequence >tr|Q5F759|Q5F759_NEIG1 Putative cytochrome OS=Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) GN=NGO1328 PE=4 SV=1 MKQLRDNKAQGSALFTLVSGIVIVIAVLYFLIKLAGSGSFGDVDATTEAATQTRIQPVGQ LTMGDGIPVGERQGEQIFGKICIQCHAADSNVPNAPKLEHNGDWAPRIAQGFDTLFQHAL NGFNAMPAKGGAADLTDQELKRAITYMANKSGGSFPNPDEAAPADNAASGTAS*(173)APADSAA PAEAKAEDKGAAAPAVGVDGKKVFEATCQVCHGGSIPGIPGIGKKDDWAPRIKKGKETLH KHALEGFNAMPAKGGNAGLSDDEVKAAVDYMANQSGAKF Sequence Around Glycosites (21 AA) PADNAASGTASAPADSAAPAE Glycosite Sequence Logo seqlogo Glycosite Sequence Logo Technique(s) used for Glycosylation Detection Immunoblotting with antiglycan antibodies Technique(s) used for Glycosylated Residue(s) Detection Ion-trap MS (mass spectrometry) with electron transfer dissociation (ETD) Protein Glycosylation- Implication Glycan Information Glycan Annotation O-AcHexDATDH (432 Da) and DATDH (229 Da) glycans. DATDH is 2,4 diacetamido-2,4,6 trideoxyhexose. Technique(s) used for Glycan Identification LC-ESI-MS/MS with CID (liquid chromatography-electrospray ionization–tandem mass spectrometry with collision-induced dissociation) Protein Glycosylation linked (PGL) gene(s) OST Gene Name NGO0085 OST NCBI Gene ID 3282309 OST GenBank Gene Sequence 3282309 OST Protein Name PglB OST UniProtKB/ SwissProt ID Q5FAE1 OST NCBI RefSeq YP_207258.1 OST EMBL-CDS AAW88846.1 OST UniProtKB Sequence >tr|Q5FAE1|Q5FAE1_NEIG1 Pilin glycosylation protein OS=Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) GN=NGO0085 PE=4 SV=1 MSKAVKRLFDIIASASGLIVLSPVFLVLIYLIRKNLGSPVFFIRERPGKDGKPFKMVKFR SMRDALDSDGIPLPDSERLTDFGKKLRATSLDELPELWNVLKGEMSLVGPRPLLMQYLPL YNKFQNRRHEMKPGITGWAQVNGRNALSWDEKFSCDVWYTDNFSFWLDMKILFLTVKKVL IKEGISAQGEATMPPFAGNRKLAVIGAGGHGKVVAELAAALGTYGEIVFLDDRTQGSVNG FPVIGTTLLLENSLSPEQFDITVAVGNNRIRRQITENAAALGFKLPVLIHPDATVSPSAI IGQGSVVMAKAVVQAGSVLKDGVIVNTAATVDHDCLLDAFVHISPGAHLSGNTRIGEESR IGTGACSRQQTTVGSGVTAGAGAVIVCDIPDGMTVAGNPAKPLTGKNPKTGTA OST EC Number (BRENDA) OST Genome Context Q5FAE1 Characterized Accessory Gene(s) PGL Additional Links CAZy Literatures Reference(s) 1) Vik, A., Aas, F.E., Anonsen, J.H., Bilsborough, S., Schneider, A., Egge-Jacobsen, W. and Koomey, M. (2009) Broad spectrum O-linked protein glycosylation in the human pathogen Neisseria gonorrhoeae. Proc Natl Acad Sci U S A, 106, 4447-4452. [PubMed: 19251655] Additional Comments Sequone feature: S/T plus low complexity region. Year of Identification 2009 Year of Validation 2009