ProGlyProt ID BC156 Organism Information Organism Name Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) Domain Bacteria Classification Family: Neisseriaceae Order: Neisseriales Class: Betaproteobacteria Division or phylum: "Proteobacteria" Taxonomic ID (NCBI) 242231 Genome Sequence (s) GeneBank AE004969.1 EMBL AE004969 Gene Information Gene Name NGO1371 NCBI Gene ID 3282544 GenBank Gene Sequence 3282544 Protein Information Protein Name CcoP UniProtKB/SwissProt ID Q5F718 NCBI RefSeq YP_208431.1 EMBL-CDS AAW90019.1 UniProtKB Sequence >tr|Q5F718|Q5F718_NEIG1 Putative cytochrome c oxidase subunit OS=Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) GN=NGO1371 PE=4 SV=1 MNATSQFTSNFWNIYIAVIVLLSFIALAWLLLSQNVVKRPKKGEEVQTTGHEWDGIAEYD NPLPRWWFWLCVLTWLFGIGYLVMYPGVGDYKGLLKWTSHNQYEKEVKKADEQYGKLYAK FADMPIEKVAKDPQAKQIAQNLFNTYCIQCHGSDAKGSKGFPNLTDSDWLWGGDPDKIHE TIEKGRVATMPAWGPALGEEGVKDVAHYVMSLSKPKGQYDEERAARGQALFSGPPANCFT CHGDKGQGIQGLGPNLTDDVWLWGGTQKSIIETITNGRSSQMPAWGHFLDKDKLHIMTAY VWGLSNKDGKAPVKKAEPAPAAEPAPSAPAEAAQAASEAKPAAAEPKAEEKAAPAAKADG KQVYETVCAACHGNAIPGIPHVGTKADWADRIKKGKDTLHKHAIEGFNTMPAKGGRGDLS DDEVKAAVDYMVNQSGGKF Sequence length 439 AA Subcellular Location Periplasm Function Cytochrome cbb3 oxidase subunit. EC=1.9.3.1. Protein Structure PDB ID Glycosylation Status Glycosylation Type O (Ser) linked Experimentally Validated Glycosite(s) in Full Length Protein S337 Experimentally Validated Glycosite(s ) in Mature Protein S337 Glycosite(s) Annotated Protein Sequence >tr|Q5F718|Q5F718_NEIG1 Putative cytochrome c oxidase subunit OS=Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) GN=NGO1371 PE=4 SV=1 MNATSQFTSNFWNIYIAVIVLLSFIALAWLLLSQNVVKRPKKGEEVQTTGHEWDGIAEYD NPLPRWWFWLCVLTWLFGIGYLVMYPGVGDYKGLLKWTSHNQYEKEVKKADEQYGKLYAK FADMPIEKVAKDPQAKQIAQNLFNTYCIQCHGSDAKGSKGFPNLTDSDWLWGGDPDKIHE TIEKGRVATMPAWGPALGEEGVKDVAHYVMSLSKPKGQYDEERAARGQALFSGPPANCFT CHGDKGQGIQGLGPNLTDDVWLWGGTQKSIIETITNGRSSQMPAWGHFLDKDKLHIMTAY VWGLSNKDGKAPVKKAEPAPAAEPAPSAPAEAAQAAS*(337)EAKPAAAEPKAEEKAAPAAKADG KQVYETVCAACHGNAIPGIPHVGTKADWADRIKKGKDTLHKHAIEGFNTMPAKGGRGDLS DDEVKAAVDYMVNQSGGKF Sequence Around Glycosites (21 AA) SAPAEAAQAASEAKPAAAEPK Glycosite Sequence Logo seqlogo Glycosite Sequence Logo Technique(s) used for Glycosylation Detection Immunoblotting with antiglycan antibodies Technique(s) used for Glycosylated Residue(s) Detection Substitution mutation analysis Protein Glycosylation- Implication Glycan Information Glycan Annotation O-AcHexDATDH (433 Da). DATDH is 2,4 diacetamido-2,4,6 trideoxyhexose. Technique(s) used for Glycan Identification LC-ESI-MS/MS with CID (liquid chromatography-electrospray ionization–tandem mass spectrometry with collision-induced dissociation) Protein Glycosylation linked (PGL) gene(s) OST Gene Name NGO0085 OST NCBI Gene ID 3282309 OST GenBank Gene Sequence 3282309 OST Protein Name PglB OST UniProtKB/ SwissProt ID Q5FAE1 OST NCBI RefSeq YP_207258.1 OST EMBL-CDS AAW88846.1 OST UniProtKB Sequence >tr|Q5FAE1|Q5FAE1_NEIG1 Pilin glycosylation protein OS=Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) GN=NGO0085 PE=4 SV=1 MSKAVKRLFDIIASASGLIVLSPVFLVLIYLIRKNLGSPVFFIRERPGKDGKPFKMVKFR SMRDALDSDGIPLPDSERLTDFGKKLRATSLDELPELWNVLKGEMSLVGPRPLLMQYLPL YNKFQNRRHEMKPGITGWAQVNGRNALSWDEKFSCDVWYTDNFSFWLDMKILFLTVKKVL IKEGISAQGEATMPPFAGNRKLAVIGAGGHGKVVAELAAALGTYGEIVFLDDRTQGSVNG FPVIGTTLLLENSLSPEQFDITVAVGNNRIRRQITENAAALGFKLPVLIHPDATVSPSAI IGQGSVVMAKAVVQAGSVLKDGVIVNTAATVDHDCLLDAFVHISPGAHLSGNTRIGEESR IGTGACSRQQTTVGSGVTAGAGAVIVCDIPDGMTVAGNPAKPLTGKNPKTGTA OST EC Number (BRENDA) OST Genome Context Q5FAE1 Characterized Accessory Gene(s) PGL Additional Links CAZy Literatures Reference(s) 1) Vik, A., Aas, F.E., Anonsen, J.H., Bilsborough, S., Schneider, A., Egge-Jacobsen, W. and Koomey, M. (2009) Broad spectrum O-linked protein glycosylation in the human pathogen Neisseria gonorrhoeae. Proc Natl Acad Sci U S A, 106, 4447-4452. [PubMed: 19251655] Additional Comments Sequone feature: S/T plus low complexity region. Year of Identification 2009 Year of Validation 2009