ProGlyProt IDBC154
Organism Information
Organism NameMycobacterium tuberculosis H37Rv
DomainBacteria
ClassificationFamily: Mycobacteriaceae
Suborder: Corynebacterineae
Order: Actinomycetales
Subclass: Actinobacteridae
Class: Actinobacteria
Division or phylum: "Actinobacteria"
Taxonomic ID (NCBI)1773
Genome Sequence (s)
GeneBankBX842573.1
EMBLBX842573
Gene Information
Gene NamesodC (Rv0432)
NCBI Gene ID886358
GenBank Gene Sequence886358
Protein Information
Protein NameSuperoxide dismutase [Cu-Zn]
UniProtKB/SwissProt IDP0A608
NCBI RefSeqNP_214946.1
EMBL-CDSCAB06572.1
UniProtKB Sequence>sp|P0A608|SODC_MYCTU Superoxide dismutase [Cu-Zn] OS=Mycobacterium tuberculosis GN=sodC PE=1 SV=1 MPKPADHRNHAAVSTSVLSALFLGAGAALLSACSSPQHASTVPGTTPSIWTGSPAPSGLS GHDEESPGAQSLTSTLTAPDGTKVATAKFEFANGYATVTIATTGVGKLTPGFHGLHIHQV GKCEPNSVAPTGGAPGNFLSAGGHYHVPGHTGTPASGDLASLQVRGDGSAMLVTTTDAFT MDDLLSGAKTAIIIHAGADNFANIPPERYVQVNGTPGPDETTLTTGDAGKRVACGVIGSG
Sequence length240 AA
Subcellular LocationAssociated with cell envelope (or membrane)
FunctionOne of two Mtb superoxide dismutases (SODs) and is a membrane-associated lipoprotein of the Cu, Zn-dependent SOD family. Contributes to the survival of Mtb in activated macrophages, suggesting a role in the defense against the oxidative burst produced in vivo. EC= 1.15.1.1
Protein Structure
PDB ID1PZS
Glycosylation Status
Glycosylation TypeO (Ser/Thr) linked
Experimentally Validated Glycosite(s) in Full Length ProteinT45, T46, S48, T51, S53, S57
Experimentally Validated Glycosite(s ) in Mature ProteinT45, T46, S48, T51, S53, S57
Glycosite(s) Annotated Protein Sequence>sp|P0A608|SODC_MYCTU Superoxide dismutase [Cu-Zn] OS=Mycobacterium tuberculosis GN=sodC PE=1 SV=1 MPKPADHRNHAAVSTSVLSALFLGAGAALLSACSSPQHASTVPGT*(45)T*(46)PS*(48)IWT*(51)GS*(53)PAPS*(57)GLS GHDEESPGAQSLTSTLTAPDGTKVATAKFEFANGYATVTIATTGVGKLTPGFHGLHIHQV GKCEPNSVAPTGGAPGNFLSAGGHYHVPGHTGTPASGDLASLQVRGDGSAMLVTTTDAFT MDDLLSGAKTAIIIHAGADNFANIPPERYVQVNGTPGPDETTLTTGDAGKRVACGVIGSG
Sequence Around Glycosites (21 AA)SPQHASTVPGTTPSIWTGSPA
PQHASTVPGTTPSIWTGSPAP
HASTVPGTTPSIWTGSPAPSG
TVPGTTPSIWTGSPAPSGLSG
PGTTPSIWTGSPAPSGLSGHD
PSIWTGSPAPSGLSGHDEESP
Glycosite Sequence Logoseqlogo
Glycosite Sequence Logo
Technique(s) used for Glycosylation DetectionConcanavalin A (ConA)-binding
Technique(s) used for Glycosylated Residue(s) DetectionLC-ESI-MS/MS (liquid chromatography-electrospray-ionization-tandem mass spectrometry) and site-directed mutagenesis (Thr to Ala)
Protein Glycosylation- ImplicationGlycosylation of this enzyme modulates proteolytic cleavage within the unstructured SodC N-terminus and influences SodC localization and stability.
Glycan Information
Glycan AnnotationLinkages: αMan-Thr/Ser.
Variable modifications with one to three hexose (mannose) units on each site. The total number of hexose units present is in the range of 6-10. The most dominant SodC glycoform was modified with nine hexose units.
Technique(s) used for Glycan IdentificationESI-MS/MS (electrospray ionization tandem mass spctrometry)
Protein Glycosylation linked (PGL) gene(s)
OST Gene Namepmt/rv1002c
OST NCBI Gene ID887882
OST GenBank Gene Sequence887882
OST Protein NameProtein O mannosyltransferase
OST UniProtKB/ SwissProt IDO05586
OST NCBI RefSeqNP_215518.1
OST EMBL-CDSCAB08157.1
OST UniProtKB Sequence>sp|O05586|Y1002_MYCTU Uncharacterized protein Rv1002c/MT1031 OS=Mycobacterium tuberculosis GN=Rv1002c PE=3 SV=2 MVPVVSPGPLVPVADFGPLDRLRGWIVTGLITLLATVTRFLNLGSLTDAGTPIFDEKHYA PQAWQVLNNHGVEDNPGYGLVVHPPVGKQLIAIGEAIFGYNGFGWRFTGALLGVVLVALV VRIVRRISRSTLVGAIAGVLLICDGVSFVTARTALLDGFLTFFVVAAFGALIVDRDQVRE RMHIALLAGRSAATVWGPRVGVRWWRFGAGVLLGLACATKWSGVYFVLFFGAMALAFDVA ARRQYQVQRPWLGTVRRDVLPSGYALGLIPFAVYLATYAPWFASETAIDRHAVGQAVGRN SVVPLPDAVRSLWHYTAKAFHFHAGLTNSAGNYHPWESKPWTWPMSLRPVLYAIDQQDVA GCGAQSCVKAEMLVGTPAMWWLAVPVLAYAGWRMFVRRDWRYAVVLVGYCAGWLPWFADI DRQMYFFYAATMAPFLVMGISLVLGDILYHPGQGSERRTLGLIVVCCYVALVVTNFAWLY PVLTGLPISQQTWNLEIWLPSWR
OST EC Number (BRENDA)2.4.1.109
OST Genome ContextO05586
Characterized Accessory Gene(s)Polyprenol-phosphate-mannose (PPM) synthase, Ppm1, is present. A second type of Ppm synthase (Rv3779 gene product) exclusive to slow-growing mycobacteria, is a membrane glycosyltransferase. It mannosylates polyprenyl-phosphates directly from GDP-mannose.
PGL Additional LinksCAZy
Literatures
Reference(s)1) Sartain, M.J. and Belisle, J.T. (2009) N-Terminal clustering of the O-glycosylation sites in the Mycobacterium tuberculosis lipoprotein SodC. Glycobiology, 19, 38-51. [PubMed: 18842962]
2) Scherman, H., Kaur, D., Pham, H., Skovierova, H., Jackson, M. and Brennan, P.J. (2009) Identification of a polyprenylphosphomannosyl synthase involved in the synthesis of mycobacterial mannosides. J Bacteriol, 191, 6769-6772. [PubMed: 19717608]
3) Spagnolo, L., Toro, I., D'Orazio, M., O'Neill, P.,
Additional CommentsSec-mediated translocation influences the O-mannosylation. Ppm1 does not discriminate between polyprenol substrates with variable chain lengths and saturation of the isoprene units.
Year of Identification2000
Year of Validation2009