ProGlyProt ID BC151 Organism Information Organism Name Listeria monocytogen Domain Bacteria Classification Family: Listeriaceae Order: Bacillales Class: Bacilli (or Firmibacteria) Division or phylum: "Firmicutes" Taxonomic ID (NCBI) 1639 Genome Sequence (s) GeneBank AL591976.1 EMBL AL591976 Gene Information Gene Name flaA ( lmo0690) NCBI Gene ID 987167 GenBank Gene Sequence 987167 Protein Information Protein Name Flagellin FlaA UniProtKB/SwissProt ID Q02551 NCBI RefSeq NP_464217.1 EMBL-CDS CAC98768.1 UniProtKB Sequence >sp|Q02551|FLAA_LISMO Flagellin OS=Listeria monocytogenes GN=flaA PE=3 SV=2 MKVNTNIISLKTQEYLRKNNEGMTQAQERLASGKRINSSLDDAAGLAVVTRMNVKSTGLD AASKNSSMGIDLLQTADSALSSMSSILQRMRQLAVQSSNGSFSDEDRKQYTAEFGSLIKE LDHVADTTNYNNIKLLDQTATGAATQVSIQASDKANDLINIDLFNAKGLSAGTITLGSGS TVAGYSALSVADADSSQQATEAIDELINNISNGRALLGAGMSRLSYNVSNVNNQSIATKA SASSIEDADMAAEMSEMTKYKILTQTSISMLSQANQTPQMLTQLINS Sequence length 287 AA Subcellular Location Secreted Function Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella. Protein Structure PDB ID Glycosylation Status Glycosylation Type O (Thr) linked Experimentally Validated Glycosite(s) in Full Length Protein T141, T145, T173, T175, S180 Experimentally Validated Glycosite(s ) in Mature Protein T141, T145, T173, T175, S180 Glycosite(s) Annotated Protein Sequence >sp|Q02551|FLAA_LISMO Flagellin OS=Listeria monocytogenes GN=flaA PE=3 SV=2 MKVNTNIISLKTQEYLRKNNEGMTQAQERLASGKRINSSLDDAAGLAVVTRMNVKSTGLD AASKNSSMGIDLLQTADSALSSMSSILQRMRQLAVQSSNGSFSDEDRKQYTAEFGSLIKE LDHVADTTNYNNIKLLDQTAT*(141)GAAT*(145)QVSIQASDKANDLINIDLFNAKGLSAGT*(173)IT*(175)LGSGS*(180) TVAGYSALSVADADSSQQATEAIDELINNISNGRALLGAGMSRLSYNVSNVNNQSIATKA SASSIEDADMAAEMSEMTKYKILTQTSISMLSQANQTPQMLTQLINS Sequence Around Glycosites (21 AA) NNIKLLDQTATGAATQVSIQA LLDQTATGAATQVSIQASDKA LFNAKGLSAGTITLGSGSTVA NAKGLSAGTITLGSGSTVAGY SAGTITLGSGSTVAGYSALSV Glycosite Sequence Logo seqlogo Glycosite Sequence Logo Technique(s) used for Glycosylation Detection DIG glycan detection Technique(s) used for Glycosylated Residue(s) Detection Alkaline hydrolysis and MS/MS (tandem mass spectrometry) Protein Glycosylation- Implication Glycan Information Glycan Annotation Linkage: β-GlcNAc-Thr/Ser. Technique(s) used for Glycan Identification Protein Glycosylation linked (PGL) gene(s) OST Gene Name OST NCBI Gene ID OST GenBank Gene Sequence OST Protein Name OST UniProtKB/ SwissProt ID OST NCBI RefSeq OST EMBL-CDS OST UniProtKB Sequence OST EC Number (BRENDA) OST Genome Context Characterized Accessory Gene(s) PGL Additional Links CAZy Literatures Reference(s) 1) Schirm, M., Kalmokoff, M., Aubry, A., Thibault, P., Sandoz, M. and Logan, S.M. (2004) Flagellin from Listeria monocytogenes is glycosylated with beta-O-linked N-acetylglucosamine. J Bacteriol, 186, 6721-6727. [PubMed: 15466023] Additional Comments Year of Identification 2004 Year of Validation 2004