| ProGlyProt ID | BC151 |
| Organism Information |
| Organism Name | Listeria monocytogen |
| Domain | Bacteria |
| Classification | Family: Listeriaceae
Order: Bacillales
Class: Bacilli (or Firmibacteria)
Division or phylum: "Firmicutes" |
| Taxonomic ID (NCBI) | 1639 |
| Genome Sequence (s) |
| GeneBank | AL591976.1 |
| EMBL | AL591976 |
| Gene Information |
| Gene Name | flaA ( lmo0690) |
| NCBI Gene ID | 987167 |
| GenBank Gene Sequence | 987167 |
| Protein Information |
| Protein Name | Flagellin FlaA |
| UniProtKB/SwissProt ID | Q02551 |
| NCBI RefSeq | NP_464217.1 |
| EMBL-CDS | CAC98768.1 |
| UniProtKB Sequence | >sp|Q02551|FLAA_LISMO Flagellin OS=Listeria monocytogenes GN=flaA PE=3 SV=2
MKVNTNIISLKTQEYLRKNNEGMTQAQERLASGKRINSSLDDAAGLAVVTRMNVKSTGLD
AASKNSSMGIDLLQTADSALSSMSSILQRMRQLAVQSSNGSFSDEDRKQYTAEFGSLIKE
LDHVADTTNYNNIKLLDQTATGAATQVSIQASDKANDLINIDLFNAKGLSAGTITLGSGS
TVAGYSALSVADADSSQQATEAIDELINNISNGRALLGAGMSRLSYNVSNVNNQSIATKA
SASSIEDADMAAEMSEMTKYKILTQTSISMLSQANQTPQMLTQLINS
|
| Sequence length | 287 AA |
| Subcellular Location | Secreted |
| Function | Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella. |
| Protein Structure |
| PDB ID | |
| Glycosylation Status |
| Glycosylation Type | O (Thr) linked |
| Experimentally Validated Glycosite(s) in Full Length Protein | T141, T145, T173, T175, S180 |
| Experimentally Validated Glycosite(s ) in Mature Protein | T141, T145, T173, T175, S180 |
| Glycosite(s) Annotated Protein Sequence | >sp|Q02551|FLAA_LISMO Flagellin OS=Listeria monocytogenes GN=flaA PE=3 SV=2
MKVNTNIISLKTQEYLRKNNEGMTQAQERLASGKRINSSLDDAAGLAVVTRMNVKSTGLD
AASKNSSMGIDLLQTADSALSSMSSILQRMRQLAVQSSNGSFSDEDRKQYTAEFGSLIKE
LDHVADTTNYNNIKLLDQTAT*(141)GAAT*(145)QVSIQASDKANDLINIDLFNAKGLSAGT*(173)IT*(175)LGSGS*(180)
TVAGYSALSVADADSSQQATEAIDELINNISNGRALLGAGMSRLSYNVSNVNNQSIATKA
SASSIEDADMAAEMSEMTKYKILTQTSISMLSQANQTPQMLTQLINS
|
| Sequence Around Glycosites (21 AA) | NNIKLLDQTATGAATQVSIQA
LLDQTATGAATQVSIQASDKA
LFNAKGLSAGTITLGSGSTVA
NAKGLSAGTITLGSGSTVAGY
SAGTITLGSGSTVAGYSALSV
|
| Glycosite Sequence Logo | seqlogo |
| Glycosite Sequence Logo |  |
| Technique(s) used for Glycosylation Detection | DIG glycan detection |
| Technique(s) used for Glycosylated Residue(s) Detection | Alkaline hydrolysis and MS/MS (tandem mass spectrometry) |
| Protein Glycosylation- Implication | |
| Glycan Information |
| Glycan Annotation | Linkage: β-GlcNAc-Thr/Ser. |
| Technique(s) used for Glycan Identification | |
| Protein Glycosylation linked (PGL) gene(s) |
| OST Gene Name | |
| OST NCBI Gene ID | |
| OST GenBank Gene Sequence | |
| OST Protein Name | |
| OST UniProtKB/ SwissProt ID | |
| OST NCBI RefSeq | |
| OST EMBL-CDS | |
| OST UniProtKB Sequence | |
| OST EC Number (BRENDA) | |
| OST Genome Context | |
| Characterized Accessory Gene(s) | |
| PGL Additional Links | CAZy |
| Literatures |
| Reference(s) | 1) Schirm, M., Kalmokoff, M., Aubry, A., Thibault, P., Sandoz, M. and Logan, S.M. (2004) Flagellin from Listeria monocytogenes is glycosylated with beta-O-linked N-acetylglucosamine. J Bacteriol, 186, 6721-6727. [PubMed: 15466023] |
| Additional Comments | |
| Year of Identification | 2004 |
| Year of Validation | 2004 |
