ProGlyProt ID BC150 Organism Information Organism Name Lactobacillus planta Domain Bacteria Classification Family: Lactobacillaceae Order: Lactobacillales Class: Bacilli (or Firmibacteria) Division or phylum: "Firmicutes" Taxonomic ID (NCBI) 1590 Genome Sequence (s) EMBL GU552553 Gene Information Gene Name gccF NCBI Gene ID Protein Information Protein Name Glycocin F UniProtKB/SwissProt ID E9K9Z1 NCBI RefSeq EMBL-CDS ADV57366.1 UniProtKB Sequence >tr|E9K9Z1|E9K9Z1_LACPL Prebacteriocin glycocin F OS=Lactobacillus plantarum GN=gccF PE=4 SV=1 MSKLVKTLTISEISKAQNNGGKPAWCWYTLAMCGAGYDSGTCDYMYSHCFGIKHHSSGSS SYHC Sequence length 64 AA Subcellular Location Secreted Function Glycocin F is a bacteriocin that possesses bacteriostatic activity. This activity is reversed by free N-acetylglucosamine. Protein Structure PDB ID Glycosylation Status Glycosylation Type O (Ser) linked and S (Cys) linked Experimentally Validated Glycosite(s) in Full Length Protein (Signal peptide: 1-21) S39, C64 Experimentally Validated Glycosite(s ) in Mature Protein S18, C43 Glycosite(s) Annotated Protein Sequence >tr|E9K9Z1|E9K9Z1_LACPL Prebacteriocin glycocin F OS=Lactobacillus plantarum GN=gccF PE=4 SV=1 MSKLVKTLTISEISKAQNNGGKPAWCWYTLAMCGAGYDS*(39)GTCDYMYSHCFGIKHHSSGSS SYHC*(64) Sequence Around Glycosites (21 AA) TLAMCGAGYDSGTCDYMYSHC HHSSGSSSYHC Glycosite Sequence Logo seqlogo Glycosite Sequence Logo Technique(s) used for Glycosylation Detection Mass difference measured and accounted for by Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) with electron capture dissociation (ECD) Technique(s) used for Glycosylated Residue(s) Detection Edman sequencing and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) Protein Glycosylation- Implication O-linked N-acetylglucosamine is required for bacteriostatic activity. Glycan Information Glycan Annotation Linkages: β-GlcNAc-Ser, HexNAc-Cys. N-Acetylglucosamine is β-O-linked to Ser18 and N-acetylhexosamine is S-linked to C-terminal Cys43. Technique(s) used for Glycan Identification N-acetyl-β-D-glucosaminidase GcnA treatment. Protein Glycosylation linked (PGL) gene(s) OST Gene Name OST NCBI Gene ID OST GenBank Gene Sequence OST Protein Name OST UniProtKB/ SwissProt ID OST NCBI RefSeq OST EMBL-CDS OST UniProtKB Sequence OST EC Number (BRENDA) OST Genome Context Characterized Accessory Gene(s) PGL Additional Links CAZy Literatures Reference(s) 1) Stepper, J., Shastri, S., Loo, T.S., Preston, J.C., Novak, P., Man, P., Moore, C.H., Havlicek, V., Patchett, M.L. and Norris, G.E. (2011) Cysteine S-glycosylation, a new post-translational modification found in glycopeptide bacteriocins. FEBS Lett, 585, 645-650. [PubMed: 21251913] 2) Venugopal, H., Edwards, P.J., Schwalbe, M., Claridge, J.K., Libich, D.S., Stepper, J., Loo, T., Patchett, M.L., Norris, G.E. and Pascal, S.M. (2011) Structural, dynamic, and chemical characterization of a no Additional Comments Year of Identification 2011 Year of Validation 2011