ProGlyProt ID BC148 Organism Information Organism Name Helicobacter pylori Domain Bacteria Classification Family: Helicobacteraceae Order: Campylobacterales Class: Epsilonproteobacteria Division or phylum: "Proteobacteria" Taxonomic ID (NCBI) 85963 Genome Sequence (s) GeneBank AE001439.1 EMBL AE001439 Gene Information Gene Name flaA (jhp_0548) NCBI Gene ID 889160 GenBank Gene Sequence 889160 Protein Information Protein Name Flagellin A (FlaA) UniProtKB/SwissProt ID P0A0S2 NCBI RefSeq NP_223266.1 EMBL-CDS AAD06133.1 UniProtKB Sequence >sp|P0A0S2|FLAA_HELPJ Flagellin A OS=Helicobacter pylori J99 GN=flaA PE=3 SV=2 MAFQVNTNINAMNAHVQSALTQNALKTSLERLSSGLRINKAADDASGMTVADSLRSQASS LGQAIANTNDGMGIIQVADKAMDEQLKILDTVKVKATQAAQDGQTTESRKAIQSDIVRLI QGLDNIGNTTTYNGQALLSGQFTNKEFQVGAYSNQSIKASIGSTTSDKIGQVRIATGALI TASGDISLTFKQVDGVNDVTLESVKVSSSAGTGIGVLAEVINKNSNRTGVKAYASVITTS DVAVQSGSLSNLTLNGIHLGNIADIKKNDSDGRLVAAINAVTSETGVEAYTDQKGRLNLR SIDGRGIEIKTDSVSNGPSALTMVNGGQDLTKGSTNYGRLSLTRLDAKSINVVSASDSQH LGFTAIGFGESQVAETTVNLRDVTGNFNANVKSASGANYNAVIASGNQSLGSGVTTLRGA MVVIDIAESAMKMLDKVRSDLGSVQNQMISTVNNISITQVNVKAAESQIRDVDFAEESAN FNKNNILAQSGSYAMSQANTVQQNILRLLT Sequence length 510 AA Subcellular Location Secreted Function Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella. Important for motility and virulence. Protein Structure PDB ID Glycosylation Status Glycosylation Type O (Ser/Thr) linked Experimentally Validated Glycosite(s) in Full Length Protein (Initiator Met removed) T181, T364, S208, S246, S354, S395, S405 Experimentally Validated Glycosite(s ) in Mature Protein T180, T363, S207, S245, S353, S394, S404 Glycosite(s) Annotated Protein Sequence >sp|P0A0S2|FLAA_HELPJ Flagellin A OS=Helicobacter pylori J99 GN=flaA PE=3 SV=2 MAFQVNTNINAMNAHVQSALTQNALKTSLERLSSGLRINKAADDASGMTVADSLRSQASS LGQAIANTNDGMGIIQVADKAMDEQLKILDTVKVKATQAAQDGQTTESRKAIQSDIVRLI QGLDNIGNTTTYNGQALLSGQFTNKEFQVGAYSNQSIKASIGSTTSDKIGQVRIATGALI T*(181)ASGDISLTFKQVDGVNDVTLESVKVSS*(208)SAGTGIGVLAEVINKNSNRTGVKAYASVITTS DVAVQS*(246)GSLSNLTLNGIHLGNIADIKKNDSDGRLVAAINAVTSETGVEAYTDQKGRLNLR SIDGRGIEIKTDSVSNGPSALTMVNGGQDLTKGSTNYGRLSLTRLDAKSINVVS*(354)ASDSQH LGFT*(364)AIGFGESQVAETTVNLRDVTGNFNANVKSAS*(395)GANYNAVIAS*(405)GNQSLGSGVTTLRGA MVVIDIAESAMKMLDKVRSDLGSVQNQMISTVNNISITQVNVKAAESQIRDVDFAEESAN FNKNNILAQSGSYAMSQANTVQQNILRLLT Sequence Around Glycosites (21 AA) QVRIATGALITASGDISLTFK SASDSQHLGFTAIGFGESQVA DVTLESVKVSSSAGTGIGVLA VITTSDVAVQSGSLSNLTLNG RLDAKSINVVSASDSQHLGFT GNFNANVKSASGANYNAVIAS SGANYNAVIASGNQSLGSGVT Glycosite Sequence Logo seqlogo Glycosite Sequence Logo Technique(s) used for Glycosylation Detection DIG Glycan/Protein Double Labelling (DIG-hydrazide labeling after periodate oxidation) followed by Western blot using anti-DIG antibodies. Technique(s) used for Glycosylated Residue(s) Detection cLC/ESMS (capillary liquid chromatography electrospray mass spectrometry) and MS/MS (tandem mass spectrometry) after β-elimination with ammonium hydroxide Protein Glycosylation- Implication Glycan Information Glycan Annotation Pseudaminic acid (Pse5Ac7Ac, 5,7-diacetamido-3,5,7,9-tetradeoxy-L-glycero-L-manno-nonulosonic acid). A total of seven O-linked Pse5Ac7Ac residues are present in FlaA protein. Technique(s) used for Glycan Identification CE-ESMS (capillary electrophoresis-electrospray mass spectrometry), tandem mass spectrometry and precursor ion scanning with fe-CID (front-end collision-induced dissociation) Protein Glycosylation linked (PGL) gene(s) OST Gene Name OST NCBI Gene ID OST GenBank Gene Sequence OST Protein Name OST UniProtKB/ SwissProt ID OST NCBI RefSeq OST EMBL-CDS OST UniProtKB Sequence OST EC Number (BRENDA) OST Genome Context Characterized Accessory Gene(s) PGL Additional Links CAZy Literatures Reference(s) 1) Schoenhofen, I.C., Lunin, V.V., Julien, J.P., Li, Y., Ajamian, E., Matte, A., Cygler, M., Brisson, J.R., Aubry, A., Logan, S.M. et al. (2006) Structural and functional characterization of PseC, an aminotransferase involved in the biosynthesis of pseudaminic acid, an essential flagellar modification in Helicobacter pylori. J Biol Chem, 281, 8907-8916. [PubMed: 16421095] 2) Schirm, M., Soo, E.C., Aubry, A.J., Austin, J., Thibault, P. and Logan, S.M. (2003) Structural, genetic and functiona Additional Comments Year of Identification 2002 Year of Validation 2003