| ProGlyProt ID | BC148 |
| Organism Information |
| Organism Name | Helicobacter pylori |
| Domain | Bacteria |
| Classification | Family: Helicobacteraceae
Order: Campylobacterales
Class: Epsilonproteobacteria
Division or phylum: "Proteobacteria" |
| Taxonomic ID (NCBI) | 85963 |
| Genome Sequence (s) |
| GeneBank | AE001439.1 |
| EMBL | AE001439 |
| Gene Information |
| Gene Name | flaA (jhp_0548) |
| NCBI Gene ID | 889160 |
| GenBank Gene Sequence | 889160 |
| Protein Information |
| Protein Name | Flagellin A (FlaA) |
| UniProtKB/SwissProt ID | P0A0S2 |
| NCBI RefSeq | NP_223266.1 |
| EMBL-CDS | AAD06133.1 |
| UniProtKB Sequence | >sp|P0A0S2|FLAA_HELPJ Flagellin A OS=Helicobacter pylori J99 GN=flaA PE=3 SV=2
MAFQVNTNINAMNAHVQSALTQNALKTSLERLSSGLRINKAADDASGMTVADSLRSQASS
LGQAIANTNDGMGIIQVADKAMDEQLKILDTVKVKATQAAQDGQTTESRKAIQSDIVRLI
QGLDNIGNTTTYNGQALLSGQFTNKEFQVGAYSNQSIKASIGSTTSDKIGQVRIATGALI
TASGDISLTFKQVDGVNDVTLESVKVSSSAGTGIGVLAEVINKNSNRTGVKAYASVITTS
DVAVQSGSLSNLTLNGIHLGNIADIKKNDSDGRLVAAINAVTSETGVEAYTDQKGRLNLR
SIDGRGIEIKTDSVSNGPSALTMVNGGQDLTKGSTNYGRLSLTRLDAKSINVVSASDSQH
LGFTAIGFGESQVAETTVNLRDVTGNFNANVKSASGANYNAVIASGNQSLGSGVTTLRGA
MVVIDIAESAMKMLDKVRSDLGSVQNQMISTVNNISITQVNVKAAESQIRDVDFAEESAN
FNKNNILAQSGSYAMSQANTVQQNILRLLT
|
| Sequence length | 510 AA |
| Subcellular Location | Secreted |
| Function | Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella. Important for motility and virulence. |
| Protein Structure |
| PDB ID | |
| Glycosylation Status |
| Glycosylation Type | O (Ser/Thr) linked |
| Experimentally Validated Glycosite(s) in Full Length Protein | (Initiator Met removed) T181, T364, S208, S246, S354, S395, S405
|
| Experimentally Validated Glycosite(s ) in Mature Protein | T180, T363, S207, S245, S353, S394, S404
|
| Glycosite(s) Annotated Protein Sequence | >sp|P0A0S2|FLAA_HELPJ Flagellin A OS=Helicobacter pylori J99 GN=flaA PE=3 SV=2
MAFQVNTNINAMNAHVQSALTQNALKTSLERLSSGLRINKAADDASGMTVADSLRSQASS
LGQAIANTNDGMGIIQVADKAMDEQLKILDTVKVKATQAAQDGQTTESRKAIQSDIVRLI
QGLDNIGNTTTYNGQALLSGQFTNKEFQVGAYSNQSIKASIGSTTSDKIGQVRIATGALI
T*(181)ASGDISLTFKQVDGVNDVTLESVKVSS*(208)SAGTGIGVLAEVINKNSNRTGVKAYASVITTS
DVAVQS*(246)GSLSNLTLNGIHLGNIADIKKNDSDGRLVAAINAVTSETGVEAYTDQKGRLNLR
SIDGRGIEIKTDSVSNGPSALTMVNGGQDLTKGSTNYGRLSLTRLDAKSINVVS*(354)ASDSQH
LGFT*(364)AIGFGESQVAETTVNLRDVTGNFNANVKSAS*(395)GANYNAVIAS*(405)GNQSLGSGVTTLRGA
MVVIDIAESAMKMLDKVRSDLGSVQNQMISTVNNISITQVNVKAAESQIRDVDFAEESAN
FNKNNILAQSGSYAMSQANTVQQNILRLLT
|
| Sequence Around Glycosites (21 AA) | QVRIATGALITASGDISLTFK
SASDSQHLGFTAIGFGESQVA
DVTLESVKVSSSAGTGIGVLA
VITTSDVAVQSGSLSNLTLNG
RLDAKSINVVSASDSQHLGFT
GNFNANVKSASGANYNAVIAS
SGANYNAVIASGNQSLGSGVT
|
| Glycosite Sequence Logo | seqlogo |
| Glycosite Sequence Logo |  |
| Technique(s) used for Glycosylation Detection | DIG Glycan/Protein Double Labelling (DIG-hydrazide labeling after periodate oxidation) followed by Western blot using anti-DIG antibodies. |
| Technique(s) used for Glycosylated Residue(s) Detection | cLC/ESMS (capillary liquid chromatography electrospray mass spectrometry) and MS/MS (tandem mass spectrometry) after β-elimination with ammonium hydroxide |
| Protein Glycosylation- Implication | |
| Glycan Information |
| Glycan Annotation | Pseudaminic acid (Pse5Ac7Ac, 5,7-diacetamido-3,5,7,9-tetradeoxy-L-glycero-L-manno-nonulosonic acid). A total of seven O-linked Pse5Ac7Ac residues are present in FlaA protein. |
| Technique(s) used for Glycan Identification | CE-ESMS (capillary electrophoresis-electrospray mass spectrometry), tandem mass spectrometry and precursor ion scanning with fe-CID (front-end collision-induced dissociation) |
| Protein Glycosylation linked (PGL) gene(s) |
| OST Gene Name | |
| OST NCBI Gene ID | |
| OST GenBank Gene Sequence | |
| OST Protein Name | |
| OST UniProtKB/ SwissProt ID | |
| OST NCBI RefSeq | |
| OST EMBL-CDS | |
| OST UniProtKB Sequence | |
| OST EC Number (BRENDA) | |
| OST Genome Context | |
| Characterized Accessory Gene(s) | |
| PGL Additional Links | CAZy |
| Literatures |
| Reference(s) | 1) Schoenhofen, I.C., Lunin, V.V., Julien, J.P., Li, Y., Ajamian, E., Matte, A., Cygler, M., Brisson, J.R., Aubry, A., Logan, S.M. et al. (2006) Structural and functional characterization of PseC, an aminotransferase involved in the biosynthesis of pseudaminic acid, an essential flagellar modification in Helicobacter pylori. J Biol Chem, 281, 8907-8916. [PubMed: 16421095]
2) Schirm, M., Soo, E.C., Aubry, A.J., Austin, J., Thibault, P. and Logan, S.M. (2003) Structural, genetic and functiona |
| Additional Comments | |
| Year of Identification | 2002 |
| Year of Validation | 2003 |
