ProGlyProt ID BC147 Organism Information Organism Name Haemophilus influenz Domain Bacteria Classification Family: Pasteurellaceae Order: Pasteurellales Class: Gammaproteobacteria Division or phylum: "Proteobacteria" Taxonomic ID (NCBI) 727 Genome Sequence (s) EMBL U08876 Gene Information Gene Name hmw1 NCBI Gene ID Protein Information Protein Name HMW1 (Adhesin) UniProtKB/SwissProt ID Q48031 NCBI RefSeq EMBL-CDS AAA20527.1 UniProtKB Sequence >tr|Q48031|Q48031_HAEIN Adhesin OS=Haemophilus influenzae GN=hmw1A PE=1 SV=1 MNKIYRLKFSKRLNALVAVSELARGCDHSTEKGSEKPARMKVRHLALKPLSAMLLSLGVT SIPQSVLASGLQGMDVVHGTATMQVDGNKTIIRNSVDAIINWKQFNIDQNEMVQFLQENN NSAVFNRVTSNQISQLKGILDSNGQVFLINPNGITIGKDAIINTNGFTASTLDISNENIK ARNFTFEQTKDKALAEIVNHGLITVGKDGSVNLIGGKVKNEGVISVNGGSISLLAGQKIT ISDIINPTITYSIAAPENEAVNLGDIFAKGGNINVRAATIRNQGKLSADSVSKDKSGNIV LSAKEGEAEIGGVISAQNQQAKGGKLMITGDKVTLKTGAVIDLSGKEGGETYLGGDERGE GKNGIQLAKKTSLEKGSTINVSGKEKGGRAIVWGDIALIDGNINAQGSGDIAKTGGFVET SGHDLFIKDNAIVDAKEWLLDPDNVSINAETAGRSNTSEDDEYTGSGNSASTPKRNKEKT TLTNTTLESILKKGTFVNITANQRIYVNSSINLSNGSLTLWSEGRSGGGVEINNDITTGD DTRGANLTIYSGGWVDVHKNISLGAQGNINITAKQDIAFEKGSNQVITGQGTITSGNQKG FRFNNVSLNGTGSGLQFTTKRTNKYAITNKFEGTLNISGKVNISMVLPKNESGYDKFKGR TYWNLTSLNVSESGEFNLTIDSRGSDSAGTLTQPYNLNGISFNKDTTFNVERNARVNFDI KAPIGINKYSSLNYASFNGNISVSGGGSVDFTLLASSSNVQTPGVVINSKYFNVSTGSSL RFKTSGSTKTGFSIEKDLTLNATGGNITLLQVEGTDGMIGKGIVAKKNITFEGGNITFGS RKAVTEIEGNVTINNNANVTLIGSDFDNHQKPLTIKKDVIINSGNLTAGGNIVNIAGNLT VESNANFKAITNFTFNVGGLFDNKGNSNISIAKGGARFKDIDNSKNLSITTNSSSTYRTI ISGNITNKNGDLNITNEGSDTEMQIGGDVSQKEGNLTISSDKINITKQITIKAGVDGENS DSDATNNANLTIKTKELKLTQDLNISGFNKAEITAKDGSDLTIGNTNSADGTNAKKVTFN QVKDSKISADGHKVTLHSKVETSGSNNNTEDSSDNNAGLTIDAKNVTVNNNITSHKAVSI SATSGEITTKTGTTINATTGNVEITAQTGSILGGIESSSGSVTLTATEGALAVSNISGNT VTVTANSGALTTLAGSTIKGTESVTTSSQSGDIGGTISGGTVEVKATESLTTQSNSKIKA TTGEANVTSATGTIGGTISGNTVNVTANAGDLTVGNGAEINATEGAATLTTSSGKLTTEA SSHITSAKGQVNLSAQDGSVAGSINAANVTLNTTGTLTTVKGSNINATSGTLVINAKDAE LNGAALGNHTVVNATNANGSGSVIATTSSRVNITGDLITINGLNIISKNGINTVLLKGVK IDVKYIQPGIASVDEVIEAKRILEKVKDLSDEEREALAKLGVSAVRFIEPNNTITVDTQN EFATRPLSRIVISEGRACFSNSDGATVCVNIADNGR Sequence length 1536 AA Subcellular Location Surface Function High-molecular weight protein (virulence exoprotein) that is secreted by the bacterial two-partner secretion pathway and mediates adherence to respiratory epithelium, an essential early step in the pathogenesis. Protein Structure PDB ID 2ODL Glycosylation Status Glycosylation Type N (Asn) linked Experimentally Validated Glycosite(s) in Full Length Protein N444, N484, N498, N546, N560, N570, N605, N609, N636, N642, N709, N773, N801, N806, N828, N835, N912, N928, N946, N952, N964, N973, N995, N1004, N1029, N1044, N1131, N1156, N1348, N1352, N1366, Experimentally Validated Glycosite(s ) in Mature Protein N444, N484, N498, N546, N560, N570, N605, N609, N636, N642, N709, N773, N801, N806, N828, N835, N912, N928, N946, N952, N964, N973, N995, N1004, N1029, N1044, N1131, N1156, N1348, N1352, N1366, Glycosite(s) Annotated Protein Sequence >tr|Q48031|Q48031_HAEIN Adhesin OS=Haemophilus influenzae GN=hmw1A PE=1 SV=1 MNKIYRLKFSKRLNALVAVSELARGCDHSTEKGSEKPARMKVRHLALKPLSAMLLSLGVT SIPQSVLASGLQGMDVVHGTATMQVDGNKTIIRNSVDAIINWKQFNIDQNEMVQFLQENN NSAVFNRVTSNQISQLKGILDSNGQVFLINPNGITIGKDAIINTNGFTASTLDISNENIK ARNFTFEQTKDKALAEIVNHGLITVGKDGSVNLIGGKVKNEGVISVNGGSISLLAGQKIT ISDIINPTITYSIAAPENEAVNLGDIFAKGGNINVRAATIRNQGKLSADSVSKDKSGNIV LSAKEGEAEIGGVISAQNQQAKGGKLMITGDKVTLKTGAVIDLSGKEGGETYLGGDERGE GKNGIQLAKKTSLEKGSTINVSGKEKGGRAIVWGDIALIDGNINAQGSGDIAKTGGFVET SGHDLFIKDNAIVDAKEWLLDPDN*(444)VSINAETAGRSNTSEDDEYTGSGNSASTPKRNKEKT TLTN*(484)TTLESILKKGTFVN*(498)ITANQRIYVNSSINLSNGSLTLWSEGRSGGGVEINNDITTGD DTRGAN*(546)LTIYSGGWVDVHKN*(560)ISLGAQGNIN*(570)ITAKQDIAFEKGSNQVITGQGTITSGNQKG FRFNN*(605)VSLN*(609)GTGSGLQFTTKRTNKYAITNKFEGTLN*(636) ISGKVN*(642)ISMVLPKNESGYDKFKGR TYWNLTSLNVSESGEFNLTIDSRGSDSAGTLTQPYNLNGISFNKDTTFN*(709)VERNARVNFDI KAPIGINKYSSLNYASFNGNISVSGGGSVDFTLLASSSNVQTPGVVINSKYFN*(773)VSTGSSL RFKTSGSTKTGFSIEKDLTLN*(801)ATGGN*(806)ITLLQVEGTDGMIGKGIVAKKN*(828)ITFEGGN*(835)ITFGS RKAVTEIEGNVTINNNANVTLIGSDFDNHQKPLTIKKDVIINSGNLTAGGNIVNIAGNLT VESNANFKAITN*(912)FTFNVGGLFDNKGNSN*(928)ISIAKGGARFKDIDNSKN*(946)LSITTN*(952)SSSTYRTI ISGN*(964)ITNKNGDLN*(973)ITNEGSDTEMQIGGDVSQKEGN*(995)LTISSDKIN*(1004)ITKQITIKAGVDGENS DSDATNNAN*(1029)LTIKTKELKLTQDLN*(1044)ISGFNKAEITAKDGSDLTIGNTNSADGTNAKKVTFN QVKDSKISADGHKVTLHSKVETSGSNNNTEDSSDNNAGLTIDAKNVTVNNN*(1131)ITSHKAVSI SATSGEITTKTGTTIN*(1156)ATTGNVEITAQTGSILGGIESSSGSVTLTATEGALAVSNISGNT VTVTANSGALTTLAGSTIKGTESVTTSSQSGDIGGTISGGTVEVKATESLTTQSNSKIKA TTGEANVTSATGTIGGTISGNTVNVTANAGDLTVGNGAEINATEGAATLTTSSGKLTTEA SSHITSAKGQVNLSAQDGSVAGSINAAN*(1348)VTLN*(1352)TTGTLTTVKGSNIN*(1366)ATSGTLVINAKDAE LNGAALGNHTVVNATNANGSGSVIATTSSRVNITGDLITINGLNIISKNGINTVLLKGVK IDVKYIQPGIASVDEVIEAKRILEKVKDLSDEEREALAKLGVSAVRFIEPNNTITVDTQN EFATRPLSRIVISEGRACFSNSDGATVCVNIADNGR Sequence Around Glycosites (21 AA) DAKEWLLDPDNVSINAETAGR KRNKEKTTLTNTTLESILKKG ESILKKGTFVNITANQRIYVN ITTGDDTRGANLTIYSGGWVD YSGGWVDVHKNISLGAQGNIN NISLGAQGNINITAKQDIAFE SGNQKGFRFNNVSLNGTGSGL KGFRFNNVSLNGTGSGLQFTT AITNKFEGTLNISGKVNISMV EGTLNISGKVNISMVLPKNES GISFNKDTTFNVERNARVNFD PGVVINSKYFNVSTGSSLRFK GFSIEKDLTLNATGGNITLLQ KDLTLNATGGNITLLQVEGTD MIGKGIVAKKNITFEGGNITF AKKNITFEGGNITFGSRKAVT ESNANFKAITNFTFNVGGLFD GGLFDNKGNSNISIAKGGARF Glycosite Sequence Logo seqlogo Glycosite Sequence Logo Technique(s) used for Glycosylation Detection Digoxygenin (DIG)-glycan detection Technique(s) used for Glycosylated Residue(s) Detection MS-MS (tandem mass spectrometry) Protein Glycosylation- Implication Glycosylation protects HMW1 against premature degradation during the process of secretion and facilitates HMW1 tethering to the bacterial surface, a prerequisite for HMW1-mediated adherence. Glycan Information Glycan Annotation Unusual carbohydrate modification includes glucose, galactose, and possibly mannose and corresponds to 7–8 kDa of the molecular mass. 31 modification sites carry 47 hexose units indicating the presence of hexose and dihexose (162-Da) sugars. HMW1C is capable of transferring glucose and galactose to HMW1 and is also able to generate hexose-hexose bonds. Technique(s) used for Glycan Identification Protein Glycosylation linked (PGL) gene(s) OST Gene Name hmw1C (R2846_0712) OST NCBI Gene ID OST GenBank Gene Sequence OST Protein Name HMW1C (ApHMW1C Actinobacillus pleuropneumoniae) OST UniProtKB/ SwissProt ID E3GU42 OST NCBI RefSeq OST EMBL-CDS ADO96126.1 OST UniProtKB Sequence >tr|E3GU42|E3GU42_HAEI2 Adhesin glycotransferase protein Hmw1C OS=Haemophilus influenzae (strain R2846 / 12) GN=hmw1C PE=4 SV=1 MTKENLQSVPQNTTASLVESNNDQTSLQILKQPPKPNLLRLEQHVAKKDYELACRELMAI LEKMDANFGGVHDIEFDAPAQLAYLPEKLLIHFATRLANAITTLFSDPELAISEEGALKM ISLQRWLTLIFASSPYVNADHILNKYNINPDSEGGFHLATDNSSIAKFCIFYLPESNVNM SLDALWAGNQQLCASLCFALQSSRFIGTASAFHKRAVVLQWFPKKLAEIANLDELPANIL HDVYMHCSYDLAKNKHDVKRPLNELVRKHILTQGWQDRYLYTLGKKDGKPVMMVLLEHFN SGHSIYRTHSTSMIAAREKFYLVGLGHEGVDNIGREVFDEFFEISSNNIMERLFFIRKQC ETFQPAVFYMPSIGMDITTIFVSNTRLAPIQAVALGHPATTHSEFIDYVIVEDDYVGSED CFSETLLRLPKDALPYVPSALAPQKVDYVLRENPEVVNIGIAATTMKLNPEFLLTLQEIR DKAKVKIHFHFALGQSTGLTHPYVKWFIESYLGDDATAHPHAPYHDYLAILRDCDMLLNP FPFGNTNGIIDMVTLGLVGVCKTGDEVHEHIDEGLFKRLGLPEWLIADTRETYIECALRL AENHQERLELRRYIIENNGLQKLFTGDPRPLGKILLKKTNEWKRKHLSKK OST EC Number (BRENDA) OST Genome Context Characterized Accessory Gene(s) HMW1C is a novel glycosyltransferase that forms both hexose-hexose and hexose-Asn bonds. PGL Additional Links CAZy Literatures Reference(s) 1) Choi, K.J., Grass, S., Paek, S., St Geme, J.W., 3rd and Yeo, H.J. (2010) The Actinobacillus pleuropneumoniae HMW1C-like glycosyltransferase mediates N-linked glycosylation of the Haemophilus influenzae HMW1 adhesin. PLoS One, 5, e15888. [PubMed: 21209858] 2) Grass, S., Lichti, C.F., Townsend, R.R., Gross, J. and St Geme, J.W., 3rd. (2010) The Haemophilus influenzae HMW1C protein is a glycosyltransferase that transfers hexose residues to asparagine sites in the HMW1 adhesin. PLoS Pathog, Additional Comments Year of Identification 2003 Year of Validation 2008