ProGlyProt ID BC146 Organism Information Organism Name Geobacillus tepidama Domain Bacteria Classification Family: Bacillaceae Order: Bacillales Class: Bacilli (or Firmibacteria) Division or phylum: "Firmicutes" Taxonomic ID (NCBI) 265948 Genome Sequence (s) EMBL AY883421 Gene Information Gene Name sgtA NCBI Gene ID Protein Information Protein Name SgtA UniProtKB/SwissProt ID Q58R21 NCBI RefSeq EMBL-CDS AAX46285.1 UniProtKB Sequence >tr|Q58R21|Q58R21_9BACI SgtA OS=Geobacillus tepidamans GN=sgtA PE=4 SV=1 MSKKKAIKLATASAIAASAFVAANPNASEAATNVDAVVSQAKAQMRNAYYTYSHTVTNTG EFADIKAVYAEYNKAKKAYADAVALVKKAGGANKDAYLADLEATYEVYITSRVIPYIDAY NYAVKLDAMRQELQAAVDAKDLAKVEELYHKISYELKSRTVILDRVYGQTTRDLLRSQFK AAAQQLRDSLIYDVTVSMKLKAAEEATNKGDLAAAEKALAEAQDALTKATAFKADLTTKQ ATVKAAYEAKVAPAVVGVSAVNSRTVQIKFNKPIDASTVLVDKGTTDTSDDTLKTTSIVF TALDGQPAISQTDIKASLSDDKKTLTLVAANSDFFTKRYVVDIKNVKTTDGKDVPAYTTT IDTTDSVRPAVLSYSYADNGLTLKVKFSEPLNSVGTVKLYDGTTEISVSPSFTAGSDEMT INLASSSVPVNKALTLKIFGAVDYNGNVINPNPAELTVMKTTVDTTKPTVQSVEAVNDKT VKVTFSEKLLGNPTIKIGGTTAASVSVDSTGLVYTATLNSAQLGIQAVEVSSYTDLAGNV GDAYTKVVELKADTTAPKLVSSQVVKINGIENLVLTFDEEVTTQNNITVVSSNDYYVDEN NVRKQVGVTLTTNSTNFKLYNPVNGKSKSVALDISSLPKGTYTVTLPTGATALVKDLASN PNAYAEGKQITFVRGTDSLTTKPALDTNVSTNGVEVVDNNTLRFTFTQNLDASALNLSNF NINGVALSKAVFDGATNKILVTLAPGANTWTGTHVITVSNIKNVSGIAMDTVTTTESMNE NVAPTFTATLTSADVIKVTFSEPVAHSTLSNAITGNNFTVKVDGTLNNVTGVYTDSAATT PVSATGNTGYKTVYLKLATPVTDLTKPITVSATNLVDVSAEGASPTVVGNTVSSDVVNVA K Sequence length 901 AA Subcellular Location Surface Function Surface layer glycoprotein Protein Structure PDB ID Glycosylation Status Glycosylation Type O (Ser) linked Experimentally Validated Glycosite(s) in Full Length Protein S792 Experimentally Validated Glycosite(s ) in Mature Protein S792 Glycosite(s) Annotated Protein Sequence >tr|Q58R21|Q58R21_9BACI SgtA OS=Geobacillus tepidamans GN=sgtA PE=4 SV=1 MSKKKAIKLATASAIAASAFVAANPNASEAATNVDAVVSQAKAQMRNAYYTYSHTVTNTG EFADIKAVYAEYNKAKKAYADAVALVKKAGGANKDAYLADLEATYEVYITSRVIPYIDAY NYAVKLDAMRQELQAAVDAKDLAKVEELYHKISYELKSRTVILDRVYGQTTRDLLRSQFK AAAQQLRDSLIYDVTVSMKLKAAEEATNKGDLAAAEKALAEAQDALTKATAFKADLTTKQ ATVKAAYEAKVAPAVVGVSAVNSRTVQIKFNKPIDASTVLVDKGTTDTSDDTLKTTSIVF TALDGQPAISQTDIKASLSDDKKTLTLVAANSDFFTKRYVVDIKNVKTTDGKDVPAYTTT IDTTDSVRPAVLSYSYADNGLTLKVKFSEPLNSVGTVKLYDGTTEISVSPSFTAGSDEMT INLASSSVPVNKALTLKIFGAVDYNGNVINPNPAELTVMKTTVDTTKPTVQSVEAVNDKT VKVTFSEKLLGNPTIKIGGTTAASVSVDSTGLVYTATLNSAQLGIQAVEVSSYTDLAGNV GDAYTKVVELKADTTAPKLVSSQVVKINGIENLVLTFDEEVTTQNNITVVSSNDYYVDEN NVRKQVGVTLTTNSTNFKLYNPVNGKSKSVALDISSLPKGTYTVTLPTGATALVKDLASN PNAYAEGKQITFVRGTDSLTTKPALDTNVSTNGVEVVDNNTLRFTFTQNLDASALNLSNF NINGVALSKAVFDGATNKILVTLAPGANTWTGTHVITVSNIKNVSGIAMDTVTTTESMNE NVAPTFTATLTS*(792)ADVIKVTFSEPVAHSTLSNAITGNNFTVKVDGTLNNVTGVYTDSAATT PVSATGNTGYKTVYLKLATPVTDLTKPITVSATNLVDVSAEGASPTVVGNTVSSDVVNVA K Sequence Around Glycosites (21 AA) VAPTFTATLTSADVIKVTFSE Glycosite Sequence Logo seqlogo Glycosite Sequence Logo Technique(s) used for Glycosylation Detection Periodic acid-Schiff (PAS) staining and chemical deglycosylation Technique(s) used for Glycosylated Residue(s) Detection ESI-QTOF-MS (electrospray ionization quadrupole-time-of-flight mass spectrometry) Protein Glycosylation- Implication Glycan Information Glycan Annotation Linkage: β-D-Gal-Ser/Thr. [→2)-α-L-Rhap-(1→3)-α-D-Fucp-(1→]n=~20. Rhamnose residue at the nonreducing end of the terminal repeating unit of the glycan chain is di-substituted. (R)-N-acetylmuramic acid found in an α-linkage to carbon 3 of the terminal rhamnose residue, serving as capping motif of an S-layer glycan. In addition, that rhamnose is substituted at position 2 with a β-N-acetylglucosamine residue. The S-layer glycan chains are bound via the trisaccharide core →2)-α-L- Technique(s) used for Glycan Identification HPAEC/PAD (high performance anion-exchange chromatography/pulsed amperometric detection), 1H and 13C NMR spectroscopy including 2D NOESY (nuclear Overhauser enhancement spectroscopy), HMBC (heteronuclear multiple bond correlation spectroscopy) and TOCSY (total correlation spectroscopy) Protein Glycosylation linked (PGL) gene(s) OST Gene Name OST NCBI Gene ID OST GenBank Gene Sequence OST Protein Name OST UniProtKB/ SwissProt ID OST NCBI RefSeq OST EMBL-CDS OST UniProtKB Sequence OST EC Number (BRENDA) OST Genome Context Characterized Accessory Gene(s) PGL Additional Links CAZy Literatures Reference(s) 1) Messner, P., Steiner, K., Zarschler, K. and Schaffer, C. (2008) S-layer nanoglycobiology of bacteria. Carbohydr Res, 343, 1934-1951. [PubMed: 18336801] 2) Zayni, S., Steiner, K., Pfostl, A., Hofinger, A., Kosma, P., Schaffer, C. and Messner, P. (2007) The dTDP-4-dehydro-6-deoxyglucose reductase encoding fcd gene is part of the surface layer glycoprotein glycosylation gene cluster of Geobacillus tepidamans GS5-97T. Glycobiology, 17, 433-443. [PubMed: 17202151] 3) Kahlig, H., Kolarich Additional Comments Year of Identification 2005 Year of Validation 2007