| ProGlyProt ID | BC145 |
| Organism Information |
| Organism Name | Geobacillus stearoth |
| Domain | Bacteria |
| Classification | Family: Bacillaceae
Order: Bacillales
Class: Bacilli (or Firmibacteria)
Division or phylum: "Firmicutes" |
| Taxonomic ID (NCBI) | 1422 |
| Genome Sequence (s) |
| EMBL | AF328862 |
| Gene Information |
| Gene Name | sgsE |
| NCBI Gene ID | |
| Protein Information |
| Protein Name | S-layer glycoprotein SgsE |
| UniProtKB/SwissProt ID | Q8VTF1 |
| NCBI RefSeq | |
| EMBL-CDS | AAL46630.1 |
| UniProtKB Sequence | >tr|Q8VTF1|Q8VTF1_BACST Surface layer glycoprotein SgsE OS=Bacillus stearothermophilus GN=sgsE PE=3 SV=1
MDKKKAVKLATASAVAASAFVAANPHTSQAATDVATVVSQAKAQMKEAYYTYSHTVTETG
QFPDIKDVYAAYNKAKQAYANAVAVVNKAGGAKKDAYLADLQATYETYVFKANPKSGEAR
VATYIDAYNYATKLDKMRQELKAAVDAKDLKKAEELYHKISYELKTRTVILDRVYGQSTR
ELLRSTFKADAQALRDSLIYDITVAMKAREAQDAVKAGNLDKAKAALDQVNQYVSKVTDA
FKAELQKAAQDAKAAYEAALPPKVESVTAVNAKTLEIKFNKAVDAATVIDNKGTSDTSDD
VVKATAITLKAIDDQVPVSTVKASLSDDKKTLKLVVDGAQFFTKRYVVDIKNVKTLDGKD
VPAYTTTIDTTDSVRPSVLSFSYADNGLTLKVKFSEPLASVGTVKLYDGTTEISVSPKFT
AGDDEMTINLASSSVPVNKDLTLKIFGAVDYNGNVINPNPAELTVKKTTVDITKPVVQSI
EAVNTKTVKVTFSEKLLSAPTIKIGGQTASVSVDSTGLVYTATLASALSKGVYAVEVSDY
KDLAGNSGDAYTKVVQLKADNTAPKFVSSQVVKINGVEHLVLTFDEEVTTGSNITVVQSS
DKYIDENNVLKAVGADLKTTSDNFKLYLPTDGKSKSVALNISSLPKGTYTVTLPNGLVSD
LADNPYAERKQITFVRGSDSLTTKPALDKDYDSNGVKADNNNELVFAFTQNLDASALNLS
NFNINGLTVTKAVFDGDTKHIRVTLAPGANTWTGTHVITISNIKNTSGLVMDTVTVNEYM
KENVAPTFTATLTSADVIRVDFSEPVANATISRALSANNFIVKVDGNVVTVSNVYEDNNA
TNLVQGSKGYKTVYLKLQSPVTDLSKPITLSATDIVDVNQTGAITDNNVVGNNVSATVVN
VAK |
| Sequence length | 903 AA |
| Subcellular Location | Surface |
| Function | |
| Protein Structure |
| PDB ID | |
| Glycosylation Status |
| Glycosylation Type | O (Ser/Thr) linked |
| Experimentally Validated Glycosite(s) in Full Length Protein | T590, T620, S794 |
| Experimentally Validated Glycosite(s ) in Mature Protein | T590, T620, S794 |
| Glycosite(s) Annotated Protein Sequence | >tr|Q8VTF1|Q8VTF1_BACST Surface layer glycoprotein SgsE OS=Bacillus stearothermophilus GN=sgsE PE=3 SV=1
MDKKKAVKLATASAVAASAFVAANPHTSQAATDVATVVSQAKAQMKEAYYTYSHTVTETG
QFPDIKDVYAAYNKAKQAYANAVAVVNKAGGAKKDAYLADLQATYETYVFKANPKSGEAR
VATYIDAYNYATKLDKMRQELKAAVDAKDLKKAEELYHKISYELKTRTVILDRVYGQSTR
ELLRSTFKADAQALRDSLIYDITVAMKAREAQDAVKAGNLDKAKAALDQVNQYVSKVTDA
FKAELQKAAQDAKAAYEAALPPKVESVTAVNAKTLEIKFNKAVDAATVIDNKGTSDTSDD
VVKATAITLKAIDDQVPVSTVKASLSDDKKTLKLVVDGAQFFTKRYVVDIKNVKTLDGKD
VPAYTTTIDTTDSVRPSVLSFSYADNGLTLKVKFSEPLASVGTVKLYDGTTEISVSPKFT
AGDDEMTINLASSSVPVNKDLTLKIFGAVDYNGNVINPNPAELTVKKTTVDITKPVVQSI
EAVNTKTVKVTFSEKLLSAPTIKIGGQTASVSVDSTGLVYTATLASALSKGVYAVEVSDY
KDLAGNSGDAYTKVVQLKADNTAPKFVSSQVVKINGVEHLVLTFDEEVTT*(590)GSNITVVQSS
DKYIDENNVLKAVGADLKTT*(620)SDNFKLYLPTDGKSKSVALNISSLPKGTYTVTLPNGLVSD
LADNPYAERKQITFVRGSDSLTTKPALDKDYDSNGVKADNNNELVFAFTQNLDASALNLS
NFNINGLTVTKAVFDGDTKHIRVTLAPGANTWTGTHVITISNIKNTSGLVMDTVTVNEYM
KENVAPTFTATLTS*(794)ADVIRVDFSEPVANATISRALSANNFIVKVDGNVVTVSNVYEDNNA
TNLVQGSKGYKTVYLKLQSPVTDLSKPITLSATDIVDVNQTGAITDNNVVGNNVSATVVN
VAK
|
| Sequence Around Glycosites (21 AA) | LVLTFDEEVTTGSNITVVQSS
LKAVGADLKTTSDNFKLYLPT
VAPTFTATLTSADVIRVDFSE
|
| Glycosite Sequence Logo | seqlogo |
| Glycosite Sequence Logo |  |
| Technique(s) used for Glycosylation Detection | Periodic acid-Schiff (PAS) staining and carbohydrate content determination using orcinol-sulfuric acid assay |
| Technique(s) used for Glycosylated Residue(s) Detection | Edman degradation (of glycopeptides and deglycosylated peptides) and nano-ESI-QTOF-MS/MS (electrospray ionization-quadrupole time of flight tandem mass spectrometry) |
| Protein Glycosylation- Implication | |
| Glycan Information |
| Glycan Annotation | Linkage: β-D-Galp-Ser/Thr.
[→2)-α-L-Rhap-(1→3)-β-L-Rhap-(1→2)-α-L-Rhap-(1→]n =13–18 (15 on average), with a 2-Omethyl group capping the terminal trisaccharide repeating unit at the non-reducing end of the glycan chains. The glycan chains are bound via the disaccharide (or trisaccharide with additional α-L-Rhap) core→3)-α-L-Rhap-(1→3)-α-L-Rhap-(1→and the linkage glycose β-D-Galp. Approx. 3.6% sugar content detected. Three inherently heterogeneic glycoprotein species o |
| Technique(s) used for Glycan Identification | HPAEC/PAD (high performance anion-exchange chromatography/pulsed amperometric detection), MALDI-TOF MS analysis, 1D and 2D 1H and 13C NMR spectroscopy including 2D NOESY (nuclear Overhauser enhancement spectroscopy) and HMBC (heteronuclear multiple bond correlation spectroscopy) |
| Protein Glycosylation linked (PGL) gene(s) |
| OST Gene Name | wsaB |
| OST NCBI Gene ID | |
| OST GenBank Gene Sequence | |
| OST Protein Name | WsaB (putative oligosaccharyl:protein transferase) |
| OST UniProtKB/ SwissProt ID | |
| OST NCBI RefSeq | |
| OST EMBL-CDS | |
| OST UniProtKB Sequence | |
| OST EC Number (BRENDA) | |
| OST Genome Context | |
| Characterized Accessory Gene(s) | Four rhamnosyltransferases are encoded in the slg gene cluster. WsaC and WsaD are both α1,3-rhamnosyltransferases, WsaE is α1,2-rhamnosyltransferase, α1,3-rhamnosyltransferase, 2-O-methyltransferase, and wsaF is α1,2-rhamnosyltransferase. Membrane-anchored WsaD initiates the reaction by transfering activated β-L-Rha to the lipid-bound galactose primer. WsaP is UDP Gal:phosphoryl-polyprenol Gal-1-phosphate transferase. |
| PGL Additional Links | CAZy |
| Literatures |
| Reference(s) | 1) Messner, P., Steiner, K., Zarschler, K. and Schaffer, C. (2008) S-layer nanoglycobiology of bacteria. Carbohydr Res, 343, 1934-1951. [PubMed: 18336801]
2) Steiner, K., Novotny, R., Werz, D.B., Zarschler, K., Seeberger, P.H., Hofinger, A., Kosma, P., Schaffer, C. and Messner, P. (2008) Molecular basis of S-layer glycoprotein glycan biosynthesis in Geobacillus stearothermophilus. J Biol Chem, 283, 21120-21133. [PubMed: 18515358]
3) Steiner, K., Novotny, R., Patel, K., Vinogradov, E., |
| Additional Comments | |
| Year of Identification | 1984 |
| Year of Validation | 2002 |
