ProGlyProt ID BC141 Organism Information Organism Name Flavobacterium columnare (formerly Flexibacter columnaris) ATCC 43622 Domain Bacteria Classification Family: Flavobacteriaceae Order: "Flavobacteriales" Class: Flavobacteria Division or phylum: "Bacteroidetes" Taxonomic ID (NCBI) 996 Genome Sequence (s) EMBL Gene Information Gene Name NCBI Gene ID Protein Information Protein Name Short glycopeptides UniProtKB/SwissProt ID NCBI RefSeq EMBL-CDS UniProtKB Sequence Val-Ala-Val-Asp-Ser-Ala-Ala (tripeptides to heptapeptides have been found with a minimal common fragment of Asp-Ser-Ala) Sequence length Subcellular Location Function Protein Structure PDB ID Glycosylation Status Glycosylation Type O (Ser) linked Experimentally Validated Glycosite(s) in Full Length Protein S5 Experimentally Validated Glycosite(s ) in Mature Protein S5 Glycosite(s) Annotated Protein Sequence VAVDS*(5)AA Sequence Around Glycosites (21 AA) VAVDAA Glycosite Sequence Logo seqlogo Glycosite Sequence Logo Technique(s) used for Glycosylation Detection NMR spectra Technique(s) used for Glycosylated Residue(s) Detection ESI Q-TOF-MS (electrospray quadrupole time of flight mass spectrometry) and sequencing Protein Glycosylation- Implication Glycan Information Glycan Annotation Linkage: Man-Ser. The glycan is an unusual hexasaccharide composed of 4-O-methyl-2-acetamido-2-deoxy-D-glucuronic acid (GlcNAcA), D-glucuronic acid (D-GlcA), 2,3-di-O-acetyl-D-xylose (D-Xyl), 2-O-methyl-D-glucuronic acid (D-GlcA), D-mannose (D-Man), and 2-O-methyl-L-rhamnose (L-Rha). Technique(s) used for Glycan Identification NMR spectra and monosaccharide analysis by GC of alditol acetates. Protein Glycosylation linked (PGL) gene(s) OST Gene Name OST NCBI Gene ID OST GenBank Gene Sequence OST Protein Name OST UniProtKB/ SwissProt ID OST NCBI RefSeq OST EMBL-CDS OST UniProtKB Sequence OST EC Number (BRENDA) OST Genome Context Characterized Accessory Gene(s) PGL Additional Links CAZy Literatures Reference(s) 1) Vinogradov, E., Perry, M.B. and Kay, W.W. (2003) The structure of the glycopeptides from the fish pathogen Flavobacterium columnare. Carbohydr Res, 338, 2653-2658. [PubMed: 14670723] Additional Comments The protein could not be identified as per the reference given. Only a heptapeptide was detected. Year of Identification 2003 Year of Validation 2003