| ProGlyProt ID | BC141 |
| Organism Information |
| Organism Name | Flavobacterium columnare (formerly Flexibacter columnaris) ATCC 43622 |
| Domain | Bacteria |
| Classification | Family: Flavobacteriaceae
Order: "Flavobacteriales"
Class: Flavobacteria
Division or phylum: "Bacteroidetes" |
| Taxonomic ID (NCBI) | 996 |
| Genome Sequence (s) |
| EMBL | |
| Gene Information |
| Gene Name | |
| NCBI Gene ID | |
| Protein Information |
| Protein Name | Short glycopeptides |
| UniProtKB/SwissProt ID | |
| NCBI RefSeq | |
| EMBL-CDS | |
| UniProtKB Sequence | Val-Ala-Val-Asp-Ser-Ala-Ala (tripeptides to heptapeptides have been found with a minimal common fragment of Asp-Ser-Ala) |
| Sequence length | |
| Subcellular Location | |
| Function | |
| Protein Structure |
| PDB ID | |
| Glycosylation Status |
| Glycosylation Type | O (Ser) linked |
| Experimentally Validated Glycosite(s) in Full Length Protein | S5 |
| Experimentally Validated Glycosite(s ) in Mature Protein | S5 |
| Glycosite(s) Annotated Protein Sequence | VAVDS*(5)AA |
| Sequence Around Glycosites (21 AA) | VAVDAA |
| Glycosite Sequence Logo | seqlogo |
| Glycosite Sequence Logo |  |
| Technique(s) used for Glycosylation Detection | NMR spectra |
| Technique(s) used for Glycosylated Residue(s) Detection | ESI Q-TOF-MS (electrospray quadrupole time of flight mass spectrometry) and sequencing |
| Protein Glycosylation- Implication | |
| Glycan Information |
| Glycan Annotation | Linkage: Man-Ser. The glycan is an unusual hexasaccharide composed of 4-O-methyl-2-acetamido-2-deoxy-D-glucuronic acid (GlcNAcA), D-glucuronic acid (D-GlcA), 2,3-di-O-acetyl-D-xylose (D-Xyl), 2-O-methyl-D-glucuronic acid (D-GlcA), D-mannose (D-Man), and 2-O-methyl-L-rhamnose (L-Rha). |
| Technique(s) used for Glycan Identification | NMR spectra and monosaccharide analysis by GC of alditol acetates. |
| Protein Glycosylation linked (PGL) gene(s) |
| OST Gene Name | |
| OST NCBI Gene ID | |
| OST GenBank Gene Sequence | |
| OST Protein Name | |
| OST UniProtKB/ SwissProt ID | |
| OST NCBI RefSeq | |
| OST EMBL-CDS | |
| OST UniProtKB Sequence | |
| OST EC Number (BRENDA) | |
| OST Genome Context | |
| Characterized Accessory Gene(s) | |
| PGL Additional Links | CAZy |
| Literatures |
| Reference(s) | 1) Vinogradov, E., Perry, M.B. and Kay, W.W. (2003) The structure of the glycopeptides from the fish pathogen Flavobacterium columnare. Carbohydr Res, 338, 2653-2658. [PubMed: 14670723] |
| Additional Comments | The protein could not be identified as per the reference given. Only a heptapeptide was detected. |
| Year of Identification | 2003 |
| Year of Validation | 2003 |
