| ProGlyProt ID | BC139 |
| Organism Information |
| Organism Name | Escherichia coli 278 |
| Domain | Bacteria |
| Classification | Family: Enterobacteriaceae
Order: "Enterobacteriales"
Class: Gammaproteobacteria
Division or phylum: "Proteobacteria" |
| Taxonomic ID (NCBI) | 562 |
| Genome Sequence (s) |
| EMBL | GU810159 |
| Gene Information |
| Gene Name | aidA (plasmid encoded) |
| NCBI Gene ID | |
| Protein Information |
| Protein Name | Diffuse Adherence Adhesin (AIDA-I) |
| UniProtKB/SwissProt ID | D7PPP4 |
| NCBI RefSeq | |
| EMBL-CDS | ADH10230.1 |
| UniProtKB Sequence | >tr|D7PPP4|D7PPP4_ECOLX Diffuse adherence adhesin OS=Escherichia coli GN=aidA PE=4 SV=1
MNKAYSIIWSHSRQAWIVASELARGHGFVLAKNTLLVLAVVSTIGNAFAVNISGTVSSGG
TVSSGETQIVYSGGETSNATVNSGGTQIVNNGGKTTATTVNSSGSQNVGTSGATISTIVN
SGGIQRVSSGGVASATNLSGGAQNIYNLGHASNTVIFSGGNQTIFSGGITDSTNISSGGQ
QRVSSGGVASNTTINSSGAQNILSGGSAISTHISSGGNQYISAGANATETIVNSGGFQRV
NSGAVATGTVLSGGTQNVSSGGSAISTSVYNSGVQTVFAGATVTDTTVNSGGNQNISSGG
IVSETTVNVSGTQNIYSGGSALSANIKGSQIVNSEGTAINTLVSDGGYQHIRNGGIASGT
IVNQSGYVNISSGGYAESTIINSGGTLRVLSDGYARGTILNNSGRENVSNGGVSYNAMIN
TGGNQYIYSDGEATAAIVNTSGFQRINSGGTASGTKLSGGNQNVSSGGKAIDAEVYSGGK
QTVYSGGEVSGTQIFNGGMVNVSGGTASGANVNLSGRLNAFAGNVVGTILNQEGRQYVYS
GATATSTVGNNEGREYVLSGGITDGTVLNSGGLQAVSSGGKASATVINEGGAQFVYDGGQ
VTGTNIKNGGTIRVDSGASALNIALSSGGNLFTSTGATVTGTNHYGSFSVSQNHASNIVL
ENGGLLAVTSGGTATDTTVNSAGRLRIDDGGTINGTTTINADGIVAGTNIQNDGNFILNL
AENYDFETELSGSGVLVKDNTGIMTYAGTLTQAQGVNVKNGGIIFDSAVVNADMAVNQNA
YINISDQATINGSVNNNGSIVINNSIINGNITNDADLSFGTAKLLSATVNGSLVNNKNII
LNPTKESAGNTLTVSNYTGTPGSVISLGGVLEGDNSLTDRLVVKGNTSGQSDIVYVNEDG
SGGQTRDGINIISVEGNSDAEFSLKNRVVAGAYDYTLQKGNESGTDNKGWYLTSHLPTSD
TRQYRPENGSYATNMALANSLFLMDLNERKQFRAMSDNTQPESASVWMKITGGRSSGKLN
DGQNKTTTNQFINQLGGDIYKFHAEQLGDFTLGIMGGYANAKGKTINYTSNKAARNTLDG
YSVGVYGTWYQNGENATGLFAETWMQYNWFNASVKGDGLEEEKYNLNGLTASAGGGYNLN
VHTWTSPEGITGEFWLQPHLQAVWMGVTPDTHQEDNGTVVQGAGKNNIQTKAGIRASWKV
KSTLDKDTGREFRPYIEANWIHNTHEFGVKMSDDSQLLSGSRNQGEIKTGIEGVITQNLS
VNGGVAYQAGGHGSNAISGALGIKYSF
|
| Sequence length | 1287 AA |
| Subcellular Location | Periplasm (Extracellular membrane associated) |
| Function | Self-associating autotransporters (SAAT). Adhesin involved in diffuse adherence. Able to confer a pattern of diffuse adherence on the surface of cultured epithelial cells.
|
| Protein Structure |
| PDB ID | |
| Glycosylation Status |
| Glycosylation Type | O (Ser) linked |
| Experimentally Validated Glycosite(s) in Full Length Protein | S102, S111, S116, S242, S252, S334, S391, S409, S540, S546, S559, S570, S577, S578, S583, T154
|
| Experimentally Validated Glycosite(s ) in Mature Protein | S102, S111, S116, S242, S252, S334, S391, S409, S540, S546, S559, S570, S577, S578, S583, T154
|
| Glycosite(s) Annotated Protein Sequence | >tr|D7PPP4|D7PPP4_ECOLX Diffuse adherence adhesin OS=Escherichia coli GN=aidA PE=4 SV=1
MNKAYSIIWSHSRQAWIVASELARGHGFVLAKNTLLVLAVVSTIGNAFAVNISGTVSSGG
TVSSGETQIVYSGGETSNATVNSGGTQIVNNGGKTTATTVNS*(102)SGSQNVGTS*(111)GATIS*(116)TIVN
SGGIQRVSSGGVASATNLSGGAQNIYNLGHASNT*(154)VIFSGGNQTIFSGGITDSTNISSGGQ
QRVSSGGVASNTTINSSGAQNILSGGSAISTHISSGGNQYISAGANATETIVNSGGFQRV
NS*(242)GAVATGTVLS*(252)GGTQNVSSGGSAISTSVYNSGVQTVFAGATVTDTTVNSGGNQNISSGG
IVSETTVNVSGTQNIYSGGSALSANIKGSQIVNS*(334)EGTAINTLVSDGGYQHIRNGGIASGT
IVNQSGYVNISSGGYAESTIINSGGTLRVLS*(391)DGYARGTILNNSGRENVS*(409)NGGVSYNAMIN
TGGNQYIYSDGEATAAIVNTSGFQRINSGGTASGTKLSGGNQNVSSGGKAIDAEVYSGGK
QTVYSGGEVSGTQIFNGGMVNVSGGTASGANVNLSGRLNAFAGNVVGTILNQEGRQYVYS*(540)GATATS*(546)TVGNNEGREYVLS*(559)GGITDGTVLNS*(570) GGLQAVS*(577)S*(578) GGKAS*(583)ATVINEGGAQFVYDGGQ
VTGTNIKNGGTIRVDSGASALNIALSSGGNLFTSTGATVTGTNHYGSFSVSQNHASNIVL
ENGGLLAVTSGGTATDTTVNSAGRLRIDDGGTINGTTTINADGIVAGTNIQNDGNFILNL
AENYDFETELSGSGVLVKDNTGIMTYAGTLTQAQGVNVKNGGIIFDSAVVNADMAVNQNA
YINISDQATINGSVNNNGSIVINNSIINGNITNDADLSFGTAKLLSATVNGSLVNNKNII
LNPTKESAGNTLTVSNYTGTPGSVISLGGVLEGDNSLTDRLVVKGNTSGQSDIVYVNEDG
SGGQTRDGINIISVEGNSDAEFSLKNRVVAGAYDYTLQKGNESGTDNKGWYLTSHLPTSD
TRQYRPENGSYATNMALANSLFLMDLNERKQFRAMSDNTQPESASVWMKITGGRSSGKLN
DGQNKTTTNQFINQLGGDIYKFHAEQLGDFTLGIMGGYANAKGKTINYTSNKAARNTLDG
YSVGVYGTWYQNGENATGLFAETWMQYNWFNASVKGDGLEEEKYNLNGLTASAGGGYNLN
VHTWTSPEGITGEFWLQPHLQAVWMGVTPDTHQEDNGTVVQGAGKNNIQTKAGIRASWKV
KSTLDKDTGREFRPYIEANWIHNTHEFGVKMSDDSQLLSGSRNQGEIKTGIEGVITQNLS
VNGGVAYQAGGHGSNAISGALGIKYSF
|
| Sequence Around Glycosites (21 AA) | GGKTTATTVNSSGSQNVGTSG
NSSGSQNVGTSGATISTIVNS
QNVGTSGATISTIVNSGGIQR
VNSGGFQRVNSGAVATGTVLS
SGAVATGTVLSGGTQNVSSGG
ANIKGSQIVNSEGTAINTLVS
INSGGTLRVLSDGYARGTILN
ILNNSGRENVSNGGVSYNAMI
LNQEGRQYVYSGATATSTVGN
QYVYSGATATSTVGNNEGREY
GNNEGREYVLSGGITDGTVLN
GGITDGTVLNSGGLQAVSSGG
VLNSGGLQAVSSGGKASATVI
LNSGGLQAVSSGGKASATVIN
LQAVSSGGKASATVINEGGAQ
NIYNLGHASNTVIFSGGNQTI
|
| Glycosite Sequence Logo | seqlogo |
| Glycosite Sequence Logo |  |
| Technique(s) used for Glycosylation Detection | Aberrant mobility on SDS-polyacrylamide gel, labelling with digoxigenin (DIG) hydrazide after periodate oxidation. The glycan was not detectable by common lectins and could not be deglycosylated by a broad spectrum of glycosidases. |
| Technique(s) used for Glycosylated Residue(s) Detection | MS-MS (tandem mass spectrometry) and N-terminal sequencing of AIDA-I peptides |
| Protein Glycosylation- Implication | Glycosylation is required to ensure a normal conformation of AIDA-I. Glycans could be involved in receptor recognition making it essential for adhesion but not for autoaggregation or biofilm formation. It also provides increased resistance to degradation. Only the glycosylated form binds to cultured epithelial cells. Further unglycosylated AIDA-I is expressed in smaller amounts than its relatively thermostable glycosylated form. Unglycosylated AIDA-I is more sensitive to proteases and induces im |
| Glycan Information |
| Glycan Annotation | Heterogenous multiple heptose residues (atleast 19 per protein molecule). |
| Technique(s) used for Glycan Identification | Gas chromatography and mass spectrometry after methanolysis and carbohydrate labelling. |
| Protein Glycosylation linked (PGL) gene(s) |
| OST Gene Name | aah (autotransporter-adhesin-heptosyltransferase), charcaterized based on gene deletion studies |
| OST NCBI Gene ID | |
| OST GenBank Gene Sequence | |
| OST Protein Name | Aah is the heptosyltransferase. Tib C (E. coli (ET |
| OST UniProtKB/ SwissProt ID | |
| OST NCBI RefSeq | |
| OST EMBL-CDS | |
| OST UniProtKB Sequence | |
| OST EC Number (BRENDA) | |
| OST Genome Context | |
| Characterized Accessory Gene(s) | |
| PGL Additional Links | CAZy |
| Literatures |
| Reference(s) | 1) Charbonneau, M.E., Girard, V., Nikolakakis, A., Campos, M., Berthiaume, F., Dumas, F., Lepine, F. and Mourez, M. (2007) O-linked glycosylation ensures the normal conformation of the autotransporter adhesin involved in diffuse adherence. J Bacteriol, 189, 8880-8889. [PubMed: 17951390]
2) Sherlock, O., Schembri, M.A., Reisner, A. and Klemm, P. (2004) Novel roles for the AIDA adhesin from diarrheagenic Escherichia coli: cell aggregation and biofilm formation. J Bacteriol, 186, 8058-8065. [P |
| Additional Comments | AIDA- I is primarily associated with pathogenic E. coli strains involved in neonatal and postweaning diarrhea in piglets.
The glycosylated peptides were identified in a region of the protein composed of imperfect 19-amino-acid repeats.
Glycosylation by heptoses represents a novel protein modification in eubacteria.
Sequon features: The N-terminal repeats of AIDA-I that contain multiple copies of the consensus sequence VXNSGG, might serve as acceptor sites for the AAH heptosyltran |
| Year of Identification | 2001 |
| Year of Validation | 2007 |
