ProGlyProt IDBC137
Organism Information
Organism NameClostridium difficile strain 630
DomainBacteria
ClassificationFamily: Clostridiaceae
Order: Clostridiales
Class: Clostridia
Division or phylum: "Firmicutes"
Taxonomic ID (NCBI)272563
Genome Sequence (s)
GeneBankAM180355.1
EMBLAM180355
Gene Information
Gene NamefliC (CD0239)
NCBI Gene ID4916757
GenBank Gene Sequence4916757
Protein Information
Protein NameFliC (Flagellin subunit)
UniProtKB/SwissProt IDQ18CX7
NCBI RefSeqYP_001086707.1
EMBL-CDSCAJ67060.1
UniProtKB Sequence>tr|Q18CX7|Q18CX7_CLOD6 Flagellin subunit OS=Clostridium difficile (strain 630) GN=fliC PE=4 SV=1 MRVNTNVSALIANNQMGRNVNGQSKSMEKLSSGVRIKRAADDAAGLAISEKMRAQIKGLD QAGRNVQDGISVVQTAEGSLEETGNILQRMRTLSLQSANEINNTEEREKIADELTQLKDE IERISSSTEFNGKKLLDGTSSTIRLQVGASYGTNVSGTSNNNNEIKIQLVNTASIMASAG ITTASIGSMKAGGTTGTDAAKTMVSSLDAALKSLNSSRAKLGAQQNRLESTQNNLNNTLE NVTAAESRIRDTDVASEMVNLSKMNILVQASQSMLAQANQQPQGVLQLLG
Sequence length 290 AA
Subcellular LocationSurface associated
FunctionForms the filaments of bacterial flagella.
Protein Structure
PDB ID
Glycosylation Status
Glycosylation TypeO (Ser/Thr) linked
Experimentally Validated Glycosite(s) in Full Length ProteinS141, S174, T183, S188, S205
Experimentally Validated Glycosite(s ) in Mature ProteinS141, S174, T183, S188, S205
Glycosite(s) Annotated Protein Sequence>tr|Q18CX7|Q18CX7_CLOD6 Flagellin subunit OS=Clostridium difficile (strain 630) GN=fliC PE=4 SV=1 MRVNTNVSALIANNQMGRNVNGQSKSMEKLSSGVRIKRAADDAAGLAISEKMRAQIKGLD QAGRNVQDGISVVQTAEGSLEETGNILQRMRTLSLQSANEINNTEEREKIADELTQLKDE IERISSSTEFNGKKLLDGTSS*(141)TIRLQVGASYGTNVSGTSNNNNEIKIQLVNTAS*(174) IMASAG ITT*(183)ASIGS*(188)MKAGGTTGTDAAKTMVS*(205)SLDAALKSLNSSRAKLGAQQNRLESTQNNLNNTLE NVTAAESRIRDTDVASEMVNLSKMNILVQASQSMLAQANQQPQGVLQLLG
Sequence Around Glycosites (21 AA)NGKKLLDGTSSTIRLQVGASY
EIKIQLVNTASIMASAGITTA
ASIMASAGITTASIGSMKAGG
SAGITTASIGSMKAGGTTGTD
TGTDAAKTMVSSLDAALKSLN
Glycosite Sequence Logoseqlogo
Glycosite Sequence Logo
Technique(s) used for Glycosylation DetectionIntact mass analysis with QTOF2-MS (hybrid quadrupole time of flight mass spectrometry)
Technique(s) used for Glycosylated Residue(s) DetectionElectron transfer dissociation (ETD) MS
Protein Glycosylation- ImplicationGlycosylation of the flagellin protein is required for proper assembly and consequent motility.
Glycan Information
Glycan Annotation(398-Da glycan) O-linked HexNAc residue, to which a methylated aspartic acid is linked via a phosphate bond. Flagellins from a number of C. difficile isolates from more recent outbreaks are modified in O linkage with a heterogeneous glycan containing up to five monosaccharide residues with masses of 204 (HexNAc), 146 (deoxyhexose), 160 (methylated deoxyhexose), and 192 (heptose).
Technique(s) used for Glycan IdentificationMS/MS (tandem mass spectrometry), nESI-feCID-MS/MS (nano-electrospray ionization–front-end collision-induced dissociation MS/MS) analyses
Protein Glycosylation linked (PGL) gene(s)
OST Gene Name
OST NCBI Gene ID
OST GenBank Gene Sequence
OST Protein Name
OST UniProtKB/ SwissProt ID
OST NCBI RefSeq
OST EMBL-CDS
OST UniProtKB Sequence
OST EC Number (BRENDA)
OST Genome Context
Characterized Accessory Gene(s)CD0240 glycosyltransferase. Its insertional mutagenesis abolished the flagellar filament production at the cell surface.
PGL Additional LinksCAZy
Literatures
Reference(s)1) Twine, S.M., Reid, C.W., Aubry, A., McMullin, D.R., Fulton, K.M., Austin, J. and Logan, S.M. (2009) Motility and flagellar glycosylation in Clostridium difficile. J Bacteriol, 191, 7050-7062. [PubMed: 19749038]
Additional Comments
Year of Identification2009
Year of Validation2009