ProGlyProt IDBC134
Organism Information
Organism NameClostridium difficile M46846
DomainBacteria
ClassificationFamily: Clostridiaceae
Order: Clostridiales
Class: Clostridia
Division or phylum: "Firmicutes"
Taxonomic ID (NCBI)1496
Genome Sequence (s)
EMBLGU048830
Gene Information
Gene NamefliC
NCBI Gene ID
Protein Information
Protein NameFliC (Flagellin)
UniProtKB/SwissProt IDD0V7K3
NCBI RefSeq
EMBL-CDSACY01344.1
UniProtKB Sequence>tr|D0V7K3|D0V7K3_CLODI Flagellin OS=Clostridium difficile GN=fliC PE=4 SV=1 MRVNTNVSALIANNQMGRNVSAQSKSMEKLSSGVRIKRAADDAAGLAISEKMRAQIKGLD QAGRNVQDGISVVQTAEGALEETGNILQRMRTLSLQSSNETNTAEERQKIADELLQLKDE VERISSSIEFNGKKLLDGSSTEIRLQVGANYGTNVAGTTNNNNEIKVALVNTSSIMSKAG ITSSTIASLNVDGASGTDAAKQMVSSLDIALKELNTSRAKLGAQQNRLESTQNNLNNTIE NVTAAESRIRDTDVASEMVNLSKMNILVQASQSMLAQANQQPQGVLQLLG
Sequence length290 AA
Subcellular LocationSurface
FunctionForms the filaments of bacterial flagella.
Protein Structure
PDB ID
Glycosylation Status
Glycosylation TypeO (Ser/Thr) linked
Experimentally Validated Glycosite(s) in Full Length ProteinT141, S174, S205
Experimentally Validated Glycosite(s ) in Mature ProteinT141, S174, S205
Glycosite(s) Annotated Protein Sequence>tr|D0V7K3|D0V7K3_CLODI Flagellin OS=Clostridium difficile GN=fliC PE=4 SV=1 MRVNTNVSALIANNQMGRNVSAQSKSMEKLSSGVRIKRAADDAAGLAISEKMRAQIKGLD QAGRNVQDGISVVQTAEGALEETGNILQRMRTLSLQSSNETNTAEERQKIADELLQLKDE VERISSSIEFNGKKLLDGSST*(141)EIRLQVGANYGTNVAGTTNNNNEIKVALVNTSS*(174) IMSKAG ITSSTIASLNVDGASGTDAAKQMVS*(205)SLDIALKELNTSRAKLGAQQNRLESTQNNLNNTIE NVTAAESRIRDTDVASEMVNLSKMNILVQASQSMLAQANQQPQGVLQLLG
Sequence Around Glycosites (21 AA)NGKKLLDGSSTEIRLQVGANF
EIKVALVNTSSIMSKAGITSS
SGTNAAKQMVSSLDVALKELN
Glycosite Sequence Logoseqlogo
Glycosite Sequence Logo
Technique(s) used for Glycosylation DetectionIntact mass analysis with QTOF2-MS (hybrid quadrupole time of flight mass spectrometry)
Technique(s) used for Glycosylated Residue(s) DetectionElectron transfer dissociation (ETD) MS
Protein Glycosylation- Implication
Glycan Information
Glycan AnnotationSeveral novel glycan moieties, ranging in mass from 494 Da to 917 Da. HexNAc and methylated deoxyhexose found.
Technique(s) used for Glycan IdentificationMS/MS (tandem mass spectrometry), nESI-feCID-MS/MS (nano-electrospray ionization–front-end collision-induced dissociation MS/MS) analyses
Protein Glycosylation linked (PGL) gene(s)
OST Gene Name
OST NCBI Gene ID
OST GenBank Gene Sequence
OST Protein Name
OST UniProtKB/ SwissProt ID
OST NCBI RefSeq
OST EMBL-CDS
OST UniProtKB Sequence
OST EC Number (BRENDA)
OST Genome Context
Characterized Accessory Gene(s)
PGL Additional LinksCAZy
Literatures
Reference(s)1) Twine, S.M., Reid, C.W., Aubry, A., McMullin, D.R., Fulton, K.M., Austin, J. and Logan, S.M. (2009) Motility and flagellar glycosylation in Clostridium difficile. J Bacteriol, 191, 7050-7062. [PubMed: 19749038]
Additional Comments
Year of Identification2009
Year of Validation2009