ProGlyProt ID BC133 Organism Information Organism Name Clostridium difficile CM-56 Domain Bacteria Classification Family: Clostridiaceae Order: Clostridiales Class: Clostridia Division or phylum: "Firmicutes" Taxonomic ID (NCBI) 1496 Genome Sequence (s) EMBL GU048827 Gene Information Gene Name fliC NCBI Gene ID Protein Information Protein Name FliC (Flagellin) UniProtKB/SwissProt ID D0V7J6 NCBI RefSeq EMBL-CDS ACY01341.1 UniProtKB Sequence >tr|D0V7J6|D0V7J6_CLODI Flagellin OS=Clostridium difficile GN=fliC PE=4 SV=1 MRVNTNVSALIANNQMGRNVNAQSKSMEKLSSGVRIKRAADDAAGLAISEKMRAQIKGLD QAGRNVQDGISVVQTAEGALEETGNILQRMRTLSVQSSNETNTAEERQKIADELLQLKDE VERISSSIEFNGKKLLDGSSTEIRLQVGANFGTNVAGTSNNNNEIKVALVNTSSIMSKAG ITSSTIASLNADGTSGTNAAKQMVSSLDVALKELNTSRAKLGAQQNRLESTQNNLNNTIE NVTAAESRIRDTDVASEMVNLSKMNILVQASQSMLAQANQQPQGVLQLLG Sequence length 290 AA Subcellular Location Surface Function Forms the filaments of bacterial flagella. Protein Structure PDB ID Glycosylation Status Glycosylation Type O (Ser/Thr) linked Experimentally Validated Glycosite(s) in Full Length Protein T141, S174, S205 Experimentally Validated Glycosite(s ) in Mature Protein T141, S174, S205 Glycosite(s) Annotated Protein Sequence >tr|D0V7J6|D0V7J6_CLODI Flagellin OS=Clostridium difficile GN=fliC PE=4 SV=1 MRVNTNVSALIANNQMGRNVNAQSKSMEKLSSGVRIKRAADDAAGLAISEKMRAQIKGLD QAGRNVQDGISVVQTAEGALEETGNILQRMRTLSVQSSNETNTAEERQKIADELLQLKDE VERISSSIEFNGKKLLDGSST*(141)EIRLQVGANFGTNVAGTSNNNNEIKVALVNTSS*(174) IMSKAG ITSSTIASLNADGTSGTNAAKQMVS*(205)SLDVALKELNTSRAKLGAQQNRLESTQNNLNNTIE NVTAAESRIRDTDVASEMVNLSKMNILVQASQSMLAQANQQPQGVLQLLG Sequence Around Glycosites (21 AA) NGKKLLDGSSTEIRLQVGANF EIKVALVNTSSIMSKAGITSS SGTNAAKQMVSSLDVALKELN Glycosite Sequence Logo seqlogo Glycosite Sequence Logo Technique(s) used for Glycosylation Detection Intact mass analysis with QTOF2-MS (hybrid quadrupole time of flight mass spectrometry) Technique(s) used for Glycosylated Residue(s) Detection Electron transfer dissociation (ETD) MS Protein Glycosylation- Implication Glycan Information Glycan Annotation Several novel glycan moieties, ranging in mass from 494 Da to 917 Da. HexNAc and methylated deoxyhexose found. Technique(s) used for Glycan Identification MS/MS (tandem mass spectrometry), nESI-feCID-MS/MS (nano-electrospray ionization–front-end collision-induced dissociation MS/MS) analyses Protein Glycosylation linked (PGL) gene(s) OST Gene Name OST NCBI Gene ID OST GenBank Gene Sequence OST Protein Name OST UniProtKB/ SwissProt ID OST NCBI RefSeq OST EMBL-CDS OST UniProtKB Sequence OST EC Number (BRENDA) OST Genome Context Characterized Accessory Gene(s) PGL Additional Links CAZy Literatures Reference(s) 1) Twine, S.M., Reid, C.W., Aubry, A., McMullin, D.R., Fulton, K.M., Austin, J. and Logan, S.M. (2009) Motility and flagellar glycosylation in Clostridium difficile. J Bacteriol, 191, 7050-7062. [PubMed: 19749038] Additional Comments Year of Identification 2009 Year of Validation 2009