| ProGlyProt ID | BC132 |
| Organism Information |
| Organism Name | Clostridium difficile BI-7 |
| Domain | Bacteria |
| Classification | Family: Clostridiaceae
Order: Clostridiales
Class: Clostridia
Division or phylum: "Firmicutes" |
| Taxonomic ID (NCBI) | 1496 |
| Genome Sequence (s) |
| EMBL | GU048825 |
| Gene Information |
| Gene Name | fliC |
| NCBI Gene ID | |
| Protein Information |
| Protein Name | FliC (Flagellin) |
| UniProtKB/SwissProt ID | D0V7J7 |
| NCBI RefSeq | |
| EMBL-CDS | ACY01339.1 |
| UniProtKB Sequence | >tr|D0V7J7|D0V7J7_CLODI Flagellin OS=Clostridium difficile GN=fliC PE=4 SV=1
MRVNTNVSALIANNQMGRNVNAQSKSMEKLSSGVRIKRAADDAAGLAISEKMRAQIKGLD
QAGRNVQDGISVVQTAEGALEETGNILQRMRTLSVQSSNETNTAEERQKIADELLQLKDE
VERISSSIEFNGKKLLDGSSTEIRLQVGANFGTNVAGTTNNNNEIKVALVNTSSIMSKAG
ITSSTIASLNADGTSGTDAAKQMVSSLDVALKELNTSRAKLGAQQNRLESTQNNLNNTIE
NVTAAESRIRDTDVASEMVNLSKMNILVQASQSMLAQANQQPQGVLQLLG
|
| Sequence length | 290 AA |
| Subcellular Location | Surface |
| Function | Forms the filaments of bacterial flagella. |
| Protein Structure |
| PDB ID | |
| Glycosylation Status |
| Glycosylation Type | O (Ser/Thr) linked |
| Experimentally Validated Glycosite(s) in Full Length Protein | T141, S174, S205 |
| Experimentally Validated Glycosite(s ) in Mature Protein | T141, S174, S205 |
| Glycosite(s) Annotated Protein Sequence | >tr|D0V7J7|D0V7J7_CLODI Flagellin OS=Clostridium difficile GN=fliC PE=4 SV=1
MRVNTNVSALIANNQMGRNVNAQSKSMEKLSSGVRIKRAADDAAGLAISEKMRAQIKGLD
QAGRNVQDGISVVQTAEGALEETGNILQRMRTLSVQSSNETNTAEERQKIADELLQLKDE
VERISSSIEFNGKKLLDGSST*(141)EIRLQVGANFGTNVAGTTNNNNEIKVALVNTSS*(174) IMSKAG
ITSSTIASLNADGTSGTDAAKQMVS*(205)SLDVALKELNTSRAKLGAQQNRLESTQNNLNNTIE
NVTAAESRIRDTDVASEMVNLSKMNILVQASQSMLAQANQQPQGVLQLLG
|
| Sequence Around Glycosites (21 AA) | NGKKLLDGSSTEIRLQVGANF
EIKVALVNTSSIMSKAGITSS
SGTNAAKQMVSSLDVALKELN
|
| Glycosite Sequence Logo | seqlogo |
| Glycosite Sequence Logo |  |
| Technique(s) used for Glycosylation Detection | Intact mass analysis with QTOF2-MS (hybrid quadrupole time of flight mass spectrometry) |
| Technique(s) used for Glycosylated Residue(s) Detection | Electron transfer dissociation (ETD) MS |
| Protein Glycosylation- Implication | |
| Glycan Information |
| Glycan Annotation | Several novel glycan moieties, ranging in mass from 494 Da to 917 Da. HexNAc and methylated deoxyhexose found. |
| Technique(s) used for Glycan Identification | MS/MS (tandem mass spectrometry), nESI-feCID-MS/MS (nano-electrospray ionization–front-end collision-induced dissociation MS/MS) analyses |
| Protein Glycosylation linked (PGL) gene(s) |
| OST Gene Name | |
| OST NCBI Gene ID | |
| OST GenBank Gene Sequence | |
| OST Protein Name | |
| OST UniProtKB/ SwissProt ID | |
| OST NCBI RefSeq | |
| OST EMBL-CDS | |
| OST UniProtKB Sequence | |
| OST EC Number (BRENDA) | |
| OST Genome Context | |
| Characterized Accessory Gene(s) | |
| PGL Additional Links | CAZy |
| Literatures |
| Reference(s) | 1) Twine, S.M., Reid, C.W., Aubry, A., McMullin, D.R., Fulton, K.M., Austin, J. and Logan, S.M. (2009) Motility and flagellar glycosylation in Clostridium difficile. J Bacteriol, 191, 7050-7062. [PubMed: 19749038] |
| Additional Comments | |
| Year of Identification | 2009 |
| Year of Validation | 2009 |
