ProGlyProt ID BC129 Organism Information Organism Name Clostridium botulinum F str. Langeland/ A str. FE9909ACS Alberta Domain Bacteria Classification Family: Clostridiaceae Order: Clostridiales Class: Clostridia Division or phylum: "Firmicutes" Taxonomic ID (NCBI) 441772 Genome Sequence (s) GeneBank CP000728.1 EMBL CP000728 Gene Information Gene Name flaA (CLI_2781) NCBI Gene ID 5404818 GenBank Gene Sequence 5404818 Protein Information Protein Name Flagellin (FlaA) UniProtKB/SwissProt ID A7GGU9 NCBI RefSeq YP_001392009.1 EMBL-CDS ABS41149.1 UniProtKB Sequence >tr|A7GGU9|A7GGU9_CLOBL Flagellin OS=Clostridium botulinum (strain Langeland / NCTC 10281 / Type F) GN=CLI_2781 PE=4 SV=1 MIINHNLNAMNAHRQMAINTGSNGKAIEKLSSGLRINRAGDDAAGLAISEKMRGQIRGLN QASRNSQDSISLIQTAEGALNETHSILQRMRELAVQSANDTNVKVDRDNLQKEVAELQNE INRIASQTQFNTKNLLNGSAKSMVFQIGANKDQVMELTIAGMGTSALKVGSINIGSGKGA KSISGMIETIDNAINTVSGERAKLGANQNRLEHTIANLDNSAENLQAAESRIRDVDMAKE MMNFTKTNILTQAAQAMLAQANQAPQGVLQLLR Sequence length 273 AA Subcellular Location Surface Function Forms the filaments of bacterial flagella. Protein Structure PDB ID Glycosylation Status Glycosylation Type O (Ser) linked Experimentally Validated Glycosite(s) in Full Length Protein S126, S139, S142, S165, S171, S176, S182 Experimentally Validated Glycosite(s ) in Mature Protein S126, S139, S142, S165, S171, S176, S182 Glycosite(s) Annotated Protein Sequence >tr|A7GGU9|A7GGU9_CLOBL Flagellin OS=Clostridium botulinum (strain Langeland / NCTC 10281 / Type F) GN=CLI_2781 PE=4 SV=1 MIINHNLNAMNAHRQMAINTGSNGKAIEKLSSGLRINRAGDDAAGLAISEKMRGQIRGLN QASRNSQDSISLIQTAEGALNETHSILQRMRELAVQSANDTNVKVDRDNLQKEVAELQNE INRIAS*(126)QTQFNTKNLLNGS*(139)AKS*(142)MVFQIGANKDQVMELTIAGMGTS*(165)ALKVGS*(171)INIGS*(176)GKGA KS*(182)ISGMIETIDNAINTVSGERAKLGANQNRLEHTIANLDNSAENLQAAESRIRDVDMAKE MMNFTKTNILTQAAQAMLAQANQAPQGVLQLLR Sequence Around Glycosites (21 AA) ELQNEINRIASQTQFNTKNLL QFNTKNLLNGSAKSMVFQIGA TKNLLNGSAKSMVFQIGANKD MELTIAGMGTSALKVGSINIG GMGTSALKVGSINIGSGKGAK ALKVGSINIGSGKGAKSISGM INIGSGKGAKSISGMIETIDN Glycosite Sequence Logo seqlogo Glycosite Sequence Logo Technique(s) used for Glycosylation Detection Mass spectrometry Technique(s) used for Glycosylated Residue(s) Detection Electron transfer dissociation (ETD) MS with and without collision-activated dissociation (CAD). Protein Glycosylation- Implication Glycan Information Glycan Annotation A novel legionaminic acid derivative (417 Da), 7-acetamido-5-(N-methyl-glutam-4-yl)-amino-3,5,7,9-tetradeoxy-D-glycero-α-D-galacto-nonulosonic acid, (αLeg5GluNMe7Ac). Technique(s) used for Glycan Identification Homonuclear and heteronuclear 1H, 13C-NMR spectra, selective TOCSY (total correlation spectroscopy), HSQC (heteronuclear single quantum coherence), HMBC (heteronuclear multiple bond coherence). Protein Glycosylation linked (PGL) gene(s) OST Gene Name OST NCBI Gene ID OST GenBank Gene Sequence OST Protein Name OST UniProtKB/ SwissProt ID OST NCBI RefSeq OST EMBL-CDS OST UniProtKB Sequence OST EC Number (BRENDA) OST Genome Context Characterized Accessory Gene(s) Information currently not available with us. PGL Additional Links CAZy Literatures Reference(s) 1) Twine, S.M., Paul, C.J., Vinogradov, E., McNally, D.J., Brisson, J.R., Mullen, J.A., McMullin, D.R., Jarrell, H.C., Austin, J.W., Kelly, J.F. et al. (2008) Flagellar glycosylation in Clostridium botulinum. FEBS J, 275, 4428-4444. [PubMed: 18671733] Additional Comments Modification by legionaminic acid (‘sialic acid-like’ nonulosonate sugar) of Gram-positive flagellar proteins is reported for the first time. Year of Identification 2008 Year of Validation 2008