| ProGlyProt ID | BC129 |
| Organism Information |
| Organism Name | Clostridium botulinum F str. Langeland/ A str. FE9909ACS Alberta |
| Domain | Bacteria |
| Classification | Family: Clostridiaceae
Order: Clostridiales
Class: Clostridia
Division or phylum: "Firmicutes" |
| Taxonomic ID (NCBI) | 441772 |
| Genome Sequence (s) |
| GeneBank | CP000728.1 |
| EMBL | CP000728 |
| Gene Information |
| Gene Name | flaA (CLI_2781) |
| NCBI Gene ID | 5404818 |
| GenBank Gene Sequence | 5404818 |
| Protein Information |
| Protein Name | Flagellin (FlaA) |
| UniProtKB/SwissProt ID | A7GGU9 |
| NCBI RefSeq | YP_001392009.1 |
| EMBL-CDS | ABS41149.1 |
| UniProtKB Sequence | >tr|A7GGU9|A7GGU9_CLOBL Flagellin OS=Clostridium botulinum (strain Langeland / NCTC 10281 / Type F) GN=CLI_2781 PE=4 SV=1
MIINHNLNAMNAHRQMAINTGSNGKAIEKLSSGLRINRAGDDAAGLAISEKMRGQIRGLN
QASRNSQDSISLIQTAEGALNETHSILQRMRELAVQSANDTNVKVDRDNLQKEVAELQNE
INRIASQTQFNTKNLLNGSAKSMVFQIGANKDQVMELTIAGMGTSALKVGSINIGSGKGA
KSISGMIETIDNAINTVSGERAKLGANQNRLEHTIANLDNSAENLQAAESRIRDVDMAKE
MMNFTKTNILTQAAQAMLAQANQAPQGVLQLLR
|
| Sequence length | 273 AA |
| Subcellular Location | Surface |
| Function | Forms the filaments of bacterial flagella. |
| Protein Structure |
| PDB ID | |
| Glycosylation Status |
| Glycosylation Type | O (Ser) linked |
| Experimentally Validated Glycosite(s) in Full Length Protein | S126, S139, S142, S165, S171, S176, S182 |
| Experimentally Validated Glycosite(s ) in Mature Protein | S126, S139, S142, S165, S171, S176, S182 |
| Glycosite(s) Annotated Protein Sequence | >tr|A7GGU9|A7GGU9_CLOBL Flagellin OS=Clostridium botulinum (strain Langeland / NCTC 10281 / Type F) GN=CLI_2781 PE=4 SV=1
MIINHNLNAMNAHRQMAINTGSNGKAIEKLSSGLRINRAGDDAAGLAISEKMRGQIRGLN
QASRNSQDSISLIQTAEGALNETHSILQRMRELAVQSANDTNVKVDRDNLQKEVAELQNE
INRIAS*(126)QTQFNTKNLLNGS*(139)AKS*(142)MVFQIGANKDQVMELTIAGMGTS*(165)ALKVGS*(171)INIGS*(176)GKGA
KS*(182)ISGMIETIDNAINTVSGERAKLGANQNRLEHTIANLDNSAENLQAAESRIRDVDMAKE
MMNFTKTNILTQAAQAMLAQANQAPQGVLQLLR
|
| Sequence Around Glycosites (21 AA) | ELQNEINRIASQTQFNTKNLL
QFNTKNLLNGSAKSMVFQIGA
TKNLLNGSAKSMVFQIGANKD
MELTIAGMGTSALKVGSINIG
GMGTSALKVGSINIGSGKGAK
ALKVGSINIGSGKGAKSISGM
INIGSGKGAKSISGMIETIDN
|
| Glycosite Sequence Logo | seqlogo |
| Glycosite Sequence Logo |  |
| Technique(s) used for Glycosylation Detection | Mass spectrometry |
| Technique(s) used for Glycosylated Residue(s) Detection | Electron transfer dissociation (ETD) MS with and without collision-activated dissociation (CAD). |
| Protein Glycosylation- Implication | |
| Glycan Information |
| Glycan Annotation | A novel legionaminic acid derivative (417 Da), 7-acetamido-5-(N-methyl-glutam-4-yl)-amino-3,5,7,9-tetradeoxy-D-glycero-α-D-galacto-nonulosonic acid, (αLeg5GluNMe7Ac). |
| Technique(s) used for Glycan Identification | Homonuclear and heteronuclear 1H, 13C-NMR spectra, selective TOCSY (total correlation spectroscopy), HSQC (heteronuclear single quantum coherence), HMBC (heteronuclear multiple bond coherence). |
| Protein Glycosylation linked (PGL) gene(s) |
| OST Gene Name | |
| OST NCBI Gene ID | |
| OST GenBank Gene Sequence | |
| OST Protein Name | |
| OST UniProtKB/ SwissProt ID | |
| OST NCBI RefSeq | |
| OST EMBL-CDS | |
| OST UniProtKB Sequence | |
| OST EC Number (BRENDA) | |
| OST Genome Context | |
| Characterized Accessory Gene(s) | Information currently not available with us. |
| PGL Additional Links | CAZy |
| Literatures |
| Reference(s) | 1) Twine, S.M., Paul, C.J., Vinogradov, E., McNally, D.J., Brisson, J.R., Mullen, J.A., McMullin, D.R., Jarrell, H.C., Austin, J.W., Kelly, J.F. et al. (2008) Flagellar glycosylation in Clostridium botulinum. FEBS J, 275, 4428-4444. [PubMed: 18671733] |
| Additional Comments | Modification by legionaminic acid (‘sialic acid-like’ nonulosonate sugar) of Gram-positive flagellar proteins is reported for the first time. |
| Year of Identification | 2008 |
| Year of Validation | 2008 |
