ProGlyProt IDBC128
Organism Information
Organism NameCampylobacter jejuni subsp. jejuni 81-176
DomainBacteria
ClassificationFamily: Campylobacteraceae
Order: Campylobacterales
Class: Epsilonproteobacteria
Division or phylum: "Proteobacteria"
Taxonomic ID (NCBI)354242
Genome Sequence (s)
GeneBankCP000538.1
EMBLCP000538
Gene Information
Gene NameflaA (CJJ81176_1339)
NCBI Gene ID4682159
GenBank Gene Sequence4682159
Protein Information
Protein NameFlagellin (FlaA)
UniProtKB/SwissProt IDQ2M5R2
NCBI RefSeqYP_001000997.1
EMBL-CDSEAQ72691.1
UniProtKB Sequence>tr|Q2M5R2|Q2M5R2_CAMJJ FlaA OS=Campylobacter jejuni subsp. jejuni serotype O:23/36 (strain 81-176) GN=flaA PE=4 SV=1 MGFRINTNVAALNAKANSDLNAKSLDASLSRLSSGLRINSAADDASGMAIADSLRSQANT LGQAISNGNDALGILQTADKAMDEQLKILDTIKTKATQAAQDGQSLKTRTMLQADINKLM EELDNIANTTSFNGKQLLSGNFTNQEFQIGASSNQTVKATIGATQSSKIGVTRFETGAQS FTSGVVGLTIKNYNGIEDFKFDNVVISTSVGTGLGALAEEINKSADKTGVRATYDVKTTG VYAIKEGTTSQEFAINGVTIGKIEYKDGDGNGSLISAINAVKDTTGVQASKDENGKLVLT SADGRGIKITGDIGVGSGILANQKENYGRLSLVKNDGRDINISGTNLSAIGMGTTDMISQ SSVSLRESKGQISATNADAMGFNSYKGGGKFVFTQNVSSISAFMSAQGSGFSRGSGFSVG SGKNLSVGLSQGIQIISSAASMSNTYVVSAGSGFSSGSGNSQFAALKTTAANTTDETAGV TTLKGAMAVMDIAETAITNLDQIRADIGSIQNQVTSTINNITVTQVNVKAAESQIRDVDF ASESANYSKANILAQSGSYAMAQANSSQQNVLRLLQ
Sequence length 576 AA
Subcellular LocationSecreted
FunctionIt is the subunit protein which is polymerized into the flagellar filaments. Motility mediated by flagella is essential for virulence.
Protein Structure
PDB ID
Glycosylation Status
Glycosylation TypeO (Ser) linked
Experimentally Validated Glycosite(s) in Full Length ProteinS207, S343, S348, T394, S398, S401, S405, S409, S418, S426, S430, S437, S441, S449, S452, S455, S458, S461, T482
Experimentally Validated Glycosite(s ) in Mature ProteinS206, S342, S347, T393, S397, S400, S404, S408, S417, S425, S429, S436, S440, S448, S451, S454, S457, S460, T481
Glycosite(s) Annotated Protein Sequence>tr|Q2M5R2|Q2M5R2_CAMJJ FlaA OS=Campylobacter jejuni subsp. jejuni serotype O:23/36 (strain 81-176) GN=flaA PE=4 SV=1 MGFRINTNVAALNAKANSDLNAKSLDASLSRLSSGLRINSAADDASGMAIADSLRSQANT LGQAISNGNDALGILQTADKAMDEQLKILDTIKTKATQAAQDGQSLKTRTMLQADINKLM EELDNIANTTSFNGKQLLSGNFTNQEFQIGASSNQTVKATIGATQSSKIGVTRFETGAQS FTSGVVGLTIKNYNGIEDFKFDNVVIS*(207)TSVGTGLGALAEEINKSADKTGVRATYDVKTTG VYAIKEGTTSQEFAINGVTIGKIEYKDGDGNGSLISAINAVKDTTGVQASKDENGKLVLT SADGRGIKITGDIGVGSGILANQKENYGRLSLVKNDGRDINIS*(343)GTNLS*(348)AIGMGTTDMISQ SSVSLRESKGQISATNADAMGFNSYKGGGKFVFT*(394)QNVS*(398)SIS*(401)AFMS*(405)AQGS*(409)GFSRGSGFS*(418)VGSGKNLS*(426)VGLS*(430) QGIQIIS*(437)SAAS*(441) MSNTYVVS*(449)AGS*(452) GFS*(455)SGS*(458) GNS*(461)QFAALKTTAANTTDETAGVTT*(482)LKGAMAVMDIAETAITNLDQIRADIGSIQNQVTSTINNITVTQVNVKAAESQIRDVDFASESANYSKANILAQSGSYAMAQANSSQQNVLRLLQ
Sequence Around Glycosites (21 AA)EDFKFDNVVISTSVGTGLGAL
VKNDGRDINISGTNLSAIGMG
RDINISGTNLSAIGMGTTDMI
SYKGGGKFVFTQNVSSISAFM
GGKFVFTQNVSSISAFMSAQG
FVFTQNVSSISAFMSAQGSGF
QNVSSISAFMSAQGSGFSRGS
SISAFMSAQGSGFSRGSGFSV
GSGFSRGSGFSVGSGKNLSVG
GFSVGSGKNLSVGLSQGIQII
GSGKNLSVGLSQGIQIISSAA
VGLSQGIQIISSAASMSNTYV
QGIQIISSAASMSNTYVVSAG
AASMSNTYVVSAGSGFSSGSG
MSNTYVVSAGSGFSSGSGNSQ
TYVVSAGSGFSSGSGNSQFAA
VSAGSGFSSGSGNSQFAALKT
GSGFSSGSGNSQFAALKTTAA
Glycosite Sequence Logoseqlogo
Glycosite Sequence Logo
Technique(s) used for Glycosylation DetectionESMS (electrospray mass spectrometry); 10%- 6.5 kDa- mass excess detected
Technique(s) used for Glycosylated Residue(s) DetectionA combination of μLC-ESMS (liquid chromatography-electrospray mass spectrometry) and MS-MS (tandem mass spectrometry) analyses; 10 out of 19 glycosylation sites were defined using nano-ESMS after base (NH4OH)-catalyzed β-elimination.
Protein Glycosylation- ImplicationGlycosylation is required for flagellar filament formation. Certain glycans mediate filament-filament interactions resulting in AAG (autoagglutination) and other glycans appear to be critical for structural subunit-subunit interactions within the filament. Modification with pseudaminic acid and derivatives is essential for targeting and/or secretion of flagellin. Also, specific structural modifications to the flagellin glycoform have been shown to be involved in the biological fitness of C. jeju
Glycan Information
Glycan AnnotationGlycan represents 10% of the total mass of the protein.
Major glycan is pseudaminic acid and its derivatives, Pse5Pr7Pr, Pse5Ac7Ac8OAc, Pse5Am7Ac.
Pse5Ac7Ac (5,7-diacetamido-3,5,7,9 - tetradeoxy-L-glycero-L-manno- nonulosonic acid), with 5-acetamidino pseudaminic acid (Pse5Am7Ac) and 5,7-N-(2,3-dihydroxyproprionyl)-pseudaminic acid (Pse5Pr7Pr) are also present. In addition, novel glycans, Pse5Am7Ac8GlnAc and Pse5Ac7Ac8OAc, have also been found. S398 and S405 carry Pse5Pr7Pr moiety whil
Technique(s) used for Glycan IdentificationNano-ESMS and NMR analysis of HPLC fractions of trypsin digested glycopeptides including COSY(correlated spectroscopy) and NOESY (nuclear Overhauser effect spectroscopy).
Protein Glycosylation linked (PGL) gene(s)
OST Gene Name
OST NCBI Gene ID
OST GenBank Gene Sequence
OST Protein Name
OST UniProtKB/ SwissProt IDA1W0B2
OST NCBI RefSeq
OST EMBL-CDS
OST UniProtKB Sequence
OST EC Number (BRENDA)
OST Genome Context
Characterized Accessory Gene(s)PseA, PseB, PseC, PseF, PseG, PseH, PseI are the enzymes required for CMP-Pse5NAc7NAc/CMP-Pse5NAc7Am production. PseB and PseC (dehydratase/aminotransferase) catalyze the first two steps of Pse5NAc7NAc (Pse) synthesis. PseI is the Pse synthase.
PGL Additional LinksCAZy
Literatures
Reference(s)1) Maita, N., Nyirenda, J., Igura, M., Kamishikiryo, J. and Kohda, D. (2010) Comparative structural biology of eubacterial and archaeal oligosaccharyltransferases. J Biol Chem, 285, 4941-4950. [PubMed: 20007322]
2) Ewing, C.P., Andreishcheva, E. and Guerry, P. (2009) Functional characterization of flagellin glycosylation in Campylobacter jejuni 81-176. J Bacteriol, 191, 7086-7093. [PubMed: 19749047]
3) McNally, D.J., Hui, J.P., Aubry, A.J., Mui, K.K., Guerry, P., Brisson, J.R., Logan,
Additional CommentsPseudaminic acid (Pse5Ac7Ac) has also been identified in the LPS of bacteria.
Pseudomonas pilin also carries pseudaminic acid like glycan (ProGlycProt ID BC166).
Sequon features: No specific sequence features except that all but one of total 19 O-glycosylated residues are restricted to the hydrophobic central, surface-exposed domain of flagellin when folded in the filament. It has been suggested that the local hydrophobicity upstream of Ser/Thr residues partially influences the site
Year of Identification2001
Year of Validation2001