| ProGlyProt ID | BC127 |
| Organism Information |
| Organism Name | Campylobacter jejuni serotype O:23/36 (strain 81-176) /JHH1 |
| Domain | Bacteria |
| Classification | Family: Campylobacteraceae
Order: Campylobacterales
Class: Epsilonproteobacteria
Division or phylum: "Proteobacteria" |
| Taxonomic ID (NCBI) | 354242 |
| Genome Sequence (s) |
| GeneBank | CP000538.1 |
| EMBL | CP000538 |
| Gene Information |
| Gene Name | JlpA (CJJ81176_1002) |
| NCBI Gene ID | 4682683 |
| GenBank Gene Sequence | 4682683 |
| Protein Information |
| Protein Name | JlpA (42-45 kDa antigen) |
| UniProtKB/SwissProt ID | A1VZX2 |
| NCBI RefSeq | YP_001000663.1 |
| EMBL-CDS | EAQ72196.1 |
| UniProtKB Sequence | >tr|A1VZX2|A1VZX2_CAMJJ Surface-exposed lipoprotein OS=Campylobacter jejuni subsp. jejuni serotype O:23/36 (strain 81-176) GN=jlpA PE=4 SV=1
MKKGIFLSIGIAVLFSACGNSIDEKTVKKYENQLNQTVKQEIASLSQDSGIKIEFSDFKC
NADGDFIACLSPNFKTLAKDNNDEYQELFQAKNIKIRSNEIYKGETNTSISIKEYYNDLF
KNQKSIQSNLVFEDFKLGEKVVSDINASLFQQDPKISSFINKLSSDSYTLSFDNSINKQE
NNYLDNLDIKFYNAKLNFNTNLNINLKEDLLNYLDSKGIKFNTQTLAMDEQAINELLNMV
NYEQASDFSNTIQKYIILNNFKIDSTLKTEGVFSSYIATAKENLQTLKAQSQNEEQALIF
DKALAILNNITQNDDYKLNLDLKFKNIPVSDYSTQGIDSIEKLSINNQDATEALKIILPF
IMFSMLMGGASF
|
| Sequence length | 372 AA |
| Subcellular Location | Surface |
| Function | Surface-associated lipoprotein that is antigenic. Promotes adherence to epithelial cells, potentially via interactions with surface-exposed hsp 90-α. Role in colonization of chicken. |
| Protein Structure |
| PDB ID | |
| Glycosylation Status |
| Glycosylation Type | N (Asn) linked |
| Experimentally Validated Glycosite(s) in Full Length Protein | N146, N107 or N146 only
|
| Experimentally Validated Glycosite(s ) in Mature Protein | N146, N107 or N146 only
|
| Glycosite(s) Annotated Protein Sequence | >tr|A1VZX2|A1VZX2_CAMJJ Surface-exposed lipoprotein OS=Campylobacter jejuni subsp. jejuni serotype O:23/36 (strain 81-176) GN=jlpA PE=4 SV=1
MKKGIFLSIGIAVLFSACGNSIDEKTVKKYENQLNQTVKQEIASLSQDSGIKIEFSDFKC
NADGDFIACLSPNFKTLAKDNNDEYQELFQAKNIKIRSNEIYKGETN*(107)TSISIKEYYNDLF
KNQKSIQSNLVFEDFKLGEKVVSDIN*(146)ASLFQQDPKISSFINKLSSDSYTLSFDNSINKQE
NNYLDNLDIKFYNAKLNFNTNLNINLKEDLLNYLDSKGIKFNTQTLAMDEQAINELLNMV
NYEQASDFSNTIQKYIILNNFKIDSTLKTEGVFSSYIATAKENLQTLKAQSQNEEQALIF
DKALAILNNITQNDDYKLNLDLKFKNIPVSDYSTQGIDSIEKLSINNQDATEALKIILPF
IMFSMLMGGASF
|
| Sequence Around Glycosites (21 AA) | RSNEIYKGETNTSISIKEYYN
KLGEKVVSDINASLFQQDPKI
|
| Glycosite Sequence Logo | seqlogo |
| Glycosite Sequence Logo |  |
| Technique(s) used for Glycosylation Detection | SBA (soybean agglutinin) affinity chromatography and detection of multiple bands on Western blot |
| Technique(s) used for Glycosylated Residue(s) Detection | ESI/MS-MS (electrospray-ionization-tandem mass spectrometry) |
| Protein Glycosylation- Implication | Glycosylation is not required for antigenicity. |
| Glycan Information |
| Glycan Annotation | Linkage: Bac-Asn.
1406 Da heptasaccharide composed of HexNAc-HexNAc-[Hex-]HexNAc-HexNAc-HexNAc-Bac-N-Asn-Xaa, where Bac is bacillosamine, 2,4-diacetamido-2,4,6-trideoxyglucopyranose. |
| Technique(s) used for Glycan Identification | 1H, 13C NMR spectroscopy- one dimensional TOCSY (total correlation spectroscopy) and NOESY (nuclear Overhauser effect spectroscopy); HMBC (heteronuclear multiple bond coherence), HMQC (heteronuclear multiple quantum correlation) spectra. |
| Protein Glycosylation linked (PGL) gene(s) |
| OST Gene Name | pglB |
| OST NCBI Gene ID | 4682880 |
| OST GenBank Gene Sequence | 4682880 |
| OST Protein Name | PglB |
| OST UniProtKB/ SwissProt ID | A1W0B2 |
| OST NCBI RefSeq | YP_001000803.1 |
| OST EMBL-CDS | EAQ72203.1 |
| OST UniProtKB Sequence | >tr|A1W0B2|A1W0B2_CAMJJ General glycosylation pathway protein OS=Campylobacter jejuni subsp. jejuni serotype O:23/36 (strain 81-176) GN=pglB PE=4 SV=1
MLKKEYLKNPYLVLFAMIVLAYVFSVLCRFYWIWWASEFNEYFFNNQLMIISNDGYAFAE
GARDMIAGFHQPNDLSYYGSSLSTLTYWLYKITPFSFESIILYMSTFLSSLVVIPIILLA
NEYKRPLMGFVAALLASIANSYYNRTMSGYYDTDMLVIVLPMFILFFMVRMILKKDFFSL
IALPLFIGIYLWWYPSSYTLNVALIGLFLIYTLIFHRKEKIFYIAVILSSLTLSNIAWFY
QSTIIVILFALFALEQKRLNFVIIGILASVTLIFLILSGGVDPILYQLKFYIFRSDESAN
LTQGFMYFNVNQTIQEVENVDLSEFMRRISGSEIVFLFSLFGFVWLLRKHKSMIMALPIL
VLGFLALKGGLRFTIYSVPVMALGFGFLLSEFKAILVKKYSQLTSNVCIVFATILTLAPV
FIHIYNYKAPTVFSQNEASLLNQLKNIANREDYVVTWWDYGYPVRYYSDVKTLVDGGKHL
GKDNFFPSFALSKDEQAAANMARLSVEYTEKSFYAPQNDILKTDILQAMMKDYNQSNVDL
FLASLSKPDFKIDTPKTRDIYLYMPARMSLIFSTVASFSFINLDTGVLDKPFTFSTAYPL
DVKNGEIYLSNGVVLSDDFRSFKIGDNVVSVNSIVEINSIKQGEYKITPIDDKAQFYIFY
LKDSAIPYAQFILMDKTMFNSAYVQMFFLGNYDKNLFDLVINSRDAKVFKLKI
|
| OST EC Number (BRENDA) | 2.4.1.119 |
| OST Genome Context | A1W0B2 |
| Characterized Accessory Gene(s) | |
| PGL Additional Links | CAZy |
| Literatures |
| Reference(s) | 1) Maita, N., Nyirenda, J., Igura, M., Kamishikiryo, J. and Kohda, D. (2010) Comparative structural biology of eubacterial and archaeal oligosaccharyltransferases. J Biol Chem, 285, 4941-4950. [PubMed: 20007322]
2) Scott, N.E., Bogema, D.R., Connolly, A.M., Falconer, L., Djordjevic, S.P. and Cordwell, S.J. (2009) Mass spectrometric characterization of the surface-associated 42 kDa lipoprotein JlpA as a glycosylated antigen in strains of Campylobacter jejuni. J Proteome Res, 8, 4654-4664. [P |
| Additional Comments | Sequon feature: The consensus sequence for the C jejuni PglB is D/E-X'-N-X"-S/T (where X' and X" stand for any amino acid except proline) which is glycosylated when located in flexible, exposed structural elements. Tripeptide sequence NLT too has been found to be modified in vitro. |
| Year of Identification | 2009 |
| Year of Validation | 2009 |
