ProGlyProt ID BC123 Organism Information Organism Name Campylobacter jejuni 11168 Domain Bacteria Classification Family: Campylobacteraceae Order: Campylobacterales Class: Epsilonproteobacteria Division or phylum: "Proteobacteria" Taxonomic ID (NCBI) 197 Genome Sequence (s) GeneBank AL111168.1 EMBL AL111168 Gene Information Gene Name flaA (Cj1339c) NCBI Gene ID 905631 GenBank Gene Sequence 905631 Protein Information Protein Name Flagellin A (FlaA) UniProtKB/SwissProt ID P56963 NCBI RefSeq YP_002344727.1 EMBL-CDS CAL35451.1 UniProtKB Sequence >sp|P56963|FLA1_CAMJE Flagellin A OS=Campylobacter jejuni GN=flaA PE=3 SV=2 MGFRINTNVAALNAKANADLNSKSLDASLSRLSSGLRINSAADDASGMAIADSLRSQANT LGQAISNGNDALGILQTADKAMDEQLKILDTIKTKATQAAQDGQSLKTRTMLQADINRLM EELDNIANTTSFNGKQLLSGNFINQEFQIGASSNQTVKATIGATQSSKIGLTRFETGGRI STSGEVQFTLKNYNGIDDFQFQKVVISTSVGTGLGALADEINKNADKTGVRATFTVETRG IAAVRAGATSDTFAINGVKIGKVDYKDGDANGALVAAINSVKDTTGVEASIDANGQLLLT SREGRGIKIDGNIGGGAFINADMKENYGRLSLVKNDGKDILISGSNLSSAGFGATQFISQ ASVSLRESKGQIDANIADAMGFGSANKGVVLGGYSSVSAYMSSAGSGFSSGSGYSVGSGK NYSTGFANAIAISAASQLSTVYNVSAGSGFSSGSTLSQFATMKTTAFGVKDETAGVTTLK GAMAVMDIAETAITNLDQIRADIGSVQNQVTSTINNITVTQVNVKAAESQIRDVDFAAES ANYSKANILAQSGSYAMAQANSVQQNVLRLLQ Sequence length 572 AA Subcellular Location Surface Function It is the subunit protein forming the flagellar filaments. Implicated in TLR-5-mediated interaction with the innate immune system. Protein Structure PDB ID Glycosylation Status Glycosylation Type O (Ser/Thr) linked Experimentally Validated Glycosite(s) in Full Length Protein S181, S207, and either T464 or T465 or both. Experimentally Validated Glycosite(s ) in Mature Protein S181, S207, and either T464 or T465 or both. Glycosite(s) Annotated Protein Sequence >sp|P56963|FLA1_CAMJE Flagellin A OS=Campylobacter jejuni GN=flaA PE=3 SV=2 MGFRINTNVAALNAKANADLNSKSLDASLSRLSSGLRINSAADDASGMAIADSLRSQANT LGQAISNGNDALGILQTADKAMDEQLKILDTIKTKATQAAQDGQSLKTRTMLQADINRLM EELDNIANTTSFNGKQLLSGNFINQEFQIGASSNQTVKATIGATQSSKIGLTRFETGGRI S*(181)TSGEVQFTLKNYNGIDDFQFQKVVIS*(207)TSVGTGLGALADEINKNADKTGVRATFTVETRG IAAVRAGATSDTFAINGVKIGKVDYKDGDANGALVAAINSVKDTTGVEASIDANGQLLLT SREGRGIKIDGNIGGGAFINADMKENYGRLSLVKNDGKDILISGSNLSSAGFGATQFISQ ASVSLRESKGQIDANIADAMGFGSANKGVVLGGYSSVSAYMSSAGSGFSSGSGYSVGSGK NYSTGFANAIAISAASQLSTVYNVSAGSGFSSGSTLSQFATMKT*(464)T*(465)AFGVKDETAGVTTLK GAMAVMDIAETAITNLDQIRADIGSVQNQVTSTINNITVTQVNVKAAESQIRDVDFAAES ANYSKANILAQSGSYAMAQANSVQQNVLRLLQ Sequence Around Glycosites (21 AA) LTRFETGGRISTSGEVQFTLK DDFQFQKVVISTSVGTGLGAL STLSQFATMKTTAFGVKDETA TLSQFATMKTTAFGVKDETAG Glycosite Sequence Logo seqlogo Glycosite Sequence Logo Technique(s) used for Glycosylation Detection ESMS (electrospray mass spectrometry) giving broad heterogeneous molecular mass envelopes with a few discrete glycoform peaks Technique(s) used for Glycosylated Residue(s) Detection Liquid chromatography electron capture dissociation (ECD) tandem mass spectrometry (MS/MS). Also β-elimination reactions. Protein Glycosylation- Implication Glycosylation is required for flagellar filament assembly. Glycan Information Glycan Annotation Two novel glycans - dimethylglyceric acid derivatives of pseudaminic acid (Pse5Ac7Ac) and 7-acetamidino pseudaminic acid are found. Ser181 and Ser207 were glycosylated by both glycans. Thr464 or Thr465 (or both, but exclusively) have been shown to be glycosylated by first glycan. Pse5Ac7Ac is also written as Pse5NAc7NAc (Pse). Technique(s) used for Glycan Identification MS/MS (tandem mass spectrometry), precursor ion scanning, and 1H, 13C NMR spectroscopy- HMBC (heteronuclear multiple bond correlation), COSY (correlation spectroscopy) Protein Glycosylation linked (PGL) gene(s) OST Gene Name OST NCBI Gene ID OST GenBank Gene Sequence OST Protein Name OST UniProtKB/ SwissProt ID OST NCBI RefSeq OST EMBL-CDS OST UniProtKB Sequence OST EC Number (BRENDA) OST Genome Context Characterized Accessory Gene(s) Information currently not available with us. PGL Additional Links CAZy Literatures Reference(s) 1) Zampronio, C.G., Blackwell, G., Penn, C.W. and Cooper, H.J. (2011) Novel Glycosylation Sites Localized in Campylobacter jejuni Flagellin FlaA by Liquid Chromatography Electron Capture Dissociation Tandem Mass Spectrometry. J Proteome Res, 10, 1238-1245. [PubMed: 21158479] 2) Maita, N., Nyirenda, J., Igura, M., Kamishikiryo, J. and Kohda, D. (2010) Comparative structural biology of eubacterial and archaeal oligosaccharyltransferases. J Biol Chem, 285, 4941-4950. [PubMed: 20007322] 3) Additional Comments Sequon feature: Both S181 and S207 are located within the motif IS*TS pointing towards a probable consensus glycosylation sequence in FlaA. Some enzymes (e.g., neuB2 gene product) have been implicated in the post translational modification of the flagellin protein (Ref. no. 4). Year of Identification 2001 Year of Validation 2011