| ProGlyProt ID | BC123 |
| Organism Information |
| Organism Name | Campylobacter jejuni 11168 |
| Domain | Bacteria |
| Classification | Family: Campylobacteraceae
Order: Campylobacterales
Class: Epsilonproteobacteria
Division or phylum: "Proteobacteria" |
| Taxonomic ID (NCBI) | 197 |
| Genome Sequence (s) |
| GeneBank | AL111168.1 |
| EMBL | AL111168 |
| Gene Information |
| Gene Name | flaA (Cj1339c) |
| NCBI Gene ID | 905631 |
| GenBank Gene Sequence | 905631 |
| Protein Information |
| Protein Name | Flagellin A (FlaA) |
| UniProtKB/SwissProt ID | P56963 |
| NCBI RefSeq | YP_002344727.1 |
| EMBL-CDS | CAL35451.1 |
| UniProtKB Sequence | >sp|P56963|FLA1_CAMJE Flagellin A OS=Campylobacter jejuni GN=flaA PE=3 SV=2
MGFRINTNVAALNAKANADLNSKSLDASLSRLSSGLRINSAADDASGMAIADSLRSQANT
LGQAISNGNDALGILQTADKAMDEQLKILDTIKTKATQAAQDGQSLKTRTMLQADINRLM
EELDNIANTTSFNGKQLLSGNFINQEFQIGASSNQTVKATIGATQSSKIGLTRFETGGRI
STSGEVQFTLKNYNGIDDFQFQKVVISTSVGTGLGALADEINKNADKTGVRATFTVETRG
IAAVRAGATSDTFAINGVKIGKVDYKDGDANGALVAAINSVKDTTGVEASIDANGQLLLT
SREGRGIKIDGNIGGGAFINADMKENYGRLSLVKNDGKDILISGSNLSSAGFGATQFISQ
ASVSLRESKGQIDANIADAMGFGSANKGVVLGGYSSVSAYMSSAGSGFSSGSGYSVGSGK
NYSTGFANAIAISAASQLSTVYNVSAGSGFSSGSTLSQFATMKTTAFGVKDETAGVTTLK
GAMAVMDIAETAITNLDQIRADIGSVQNQVTSTINNITVTQVNVKAAESQIRDVDFAAES
ANYSKANILAQSGSYAMAQANSVQQNVLRLLQ
|
| Sequence length | 572 AA |
| Subcellular Location | Surface |
| Function | It is the subunit protein forming the flagellar filaments. Implicated in TLR-5-mediated interaction with the innate immune system. |
| Protein Structure |
| PDB ID | |
| Glycosylation Status |
| Glycosylation Type | O (Ser/Thr) linked |
| Experimentally Validated Glycosite(s) in Full Length Protein | S181, S207, and either T464 or T465 or both. |
| Experimentally Validated Glycosite(s ) in Mature Protein | S181, S207, and either T464 or T465 or both. |
| Glycosite(s) Annotated Protein Sequence | >sp|P56963|FLA1_CAMJE Flagellin A OS=Campylobacter jejuni GN=flaA PE=3 SV=2
MGFRINTNVAALNAKANADLNSKSLDASLSRLSSGLRINSAADDASGMAIADSLRSQANT
LGQAISNGNDALGILQTADKAMDEQLKILDTIKTKATQAAQDGQSLKTRTMLQADINRLM
EELDNIANTTSFNGKQLLSGNFINQEFQIGASSNQTVKATIGATQSSKIGLTRFETGGRI
S*(181)TSGEVQFTLKNYNGIDDFQFQKVVIS*(207)TSVGTGLGALADEINKNADKTGVRATFTVETRG
IAAVRAGATSDTFAINGVKIGKVDYKDGDANGALVAAINSVKDTTGVEASIDANGQLLLT
SREGRGIKIDGNIGGGAFINADMKENYGRLSLVKNDGKDILISGSNLSSAGFGATQFISQ
ASVSLRESKGQIDANIADAMGFGSANKGVVLGGYSSVSAYMSSAGSGFSSGSGYSVGSGK
NYSTGFANAIAISAASQLSTVYNVSAGSGFSSGSTLSQFATMKT*(464)T*(465)AFGVKDETAGVTTLK
GAMAVMDIAETAITNLDQIRADIGSVQNQVTSTINNITVTQVNVKAAESQIRDVDFAAES
ANYSKANILAQSGSYAMAQANSVQQNVLRLLQ
|
| Sequence Around Glycosites (21 AA) | LTRFETGGRISTSGEVQFTLK
DDFQFQKVVISTSVGTGLGAL
STLSQFATMKTTAFGVKDETA
TLSQFATMKTTAFGVKDETAG
|
| Glycosite Sequence Logo | seqlogo |
| Glycosite Sequence Logo |  |
| Technique(s) used for Glycosylation Detection | ESMS (electrospray mass spectrometry) giving broad heterogeneous molecular mass envelopes with a few discrete glycoform peaks |
| Technique(s) used for Glycosylated Residue(s) Detection | Liquid chromatography electron capture dissociation (ECD) tandem mass spectrometry (MS/MS). Also β-elimination reactions. |
| Protein Glycosylation- Implication | Glycosylation is required for flagellar filament assembly. |
| Glycan Information |
| Glycan Annotation | Two novel glycans - dimethylglyceric acid derivatives of pseudaminic acid (Pse5Ac7Ac) and 7-acetamidino pseudaminic acid are found. Ser181 and Ser207 were glycosylated by both glycans. Thr464 or Thr465 (or both, but exclusively) have been shown to be glycosylated by first glycan. Pse5Ac7Ac is also written as Pse5NAc7NAc (Pse). |
| Technique(s) used for Glycan Identification | MS/MS (tandem mass spectrometry), precursor ion scanning, and 1H, 13C NMR spectroscopy- HMBC (heteronuclear multiple bond correlation), COSY (correlation spectroscopy) |
| Protein Glycosylation linked (PGL) gene(s) |
| OST Gene Name | |
| OST NCBI Gene ID | |
| OST GenBank Gene Sequence | |
| OST Protein Name | |
| OST UniProtKB/ SwissProt ID | |
| OST NCBI RefSeq | |
| OST EMBL-CDS | |
| OST UniProtKB Sequence | |
| OST EC Number (BRENDA) | |
| OST Genome Context | |
| Characterized Accessory Gene(s) | Information currently not available with us. |
| PGL Additional Links | CAZy |
| Literatures |
| Reference(s) | 1) Zampronio, C.G., Blackwell, G., Penn, C.W. and Cooper, H.J. (2011) Novel Glycosylation Sites Localized in Campylobacter jejuni Flagellin FlaA by Liquid Chromatography Electron Capture Dissociation Tandem Mass Spectrometry. J Proteome Res, 10, 1238-1245. [PubMed: 21158479]
2) Maita, N., Nyirenda, J., Igura, M., Kamishikiryo, J. and Kohda, D. (2010) Comparative structural biology of eubacterial and archaeal oligosaccharyltransferases. J Biol Chem, 285, 4941-4950. [PubMed: 20007322]
3) |
| Additional Comments | Sequon feature: Both S181 and S207 are located within the motif IS*TS pointing towards a probable consensus glycosylation sequence in FlaA.
Some enzymes (e.g., neuB2 gene product) have been implicated in the post translational modification of the flagellin protein (Ref. no. 4). |
| Year of Identification | 2001 |
| Year of Validation | 2011 |
