ProGlyProt ID BC122 Organism Information Organism Name Campylobacter jejuni NCTC 11168 serotype O:2 Domain Bacteria Classification Family: Campylobacteraceae Order: Campylobacterales Class: Epsilonproteobacteria Division or phylum: "Proteobacteria" Taxonomic ID (NCBI) 197 Genome Sequence (s) GeneBank AL111168.1 EMBL AL111168 Gene Information Gene Name Cj1496c NCBI Gene ID 906007 GenBank Gene Sequence 906007 Protein Information Protein Name Cj1496c UniProtKB/SwissProt ID Q0P8C1 NCBI RefSeq YP_002344876.1 EMBL-CDS CAL35603.1 UniProtKB Sequence >tr|Q0P8C1|Q0P8C1_CAMJE Putative periplasmic protein OS=Campylobacter jejuni GN=Cj1496c PE=1 SV=1 MIKKFILLVFISSVVFGAEQDCEQYFEARKAQIELQTREFDEARQSLEAYKASFEALQKE RLENLEKKEAEVNATLAKIEELKLENARLVEEQQKILNSINDKTQGRVKEIYSQMKDAAI ADVLSQMDAEDASKIMLSLESRKISGVLSKMDPKKASELTLLLKNLDNNASN Sequence length 172 AA Subcellular Location Priplasm Function Required for the attachment and/or invasion to INT-407 intestinal epithelial cells and the colonization of the chick gastrointestinal tract. Protein Structure PDB ID Glycosylation Status Glycosylation Type N (Asn) linked Experimentally Validated Glycosite(s) in Full Length Protein N73, N169 Experimentally Validated Glycosite(s ) in Mature Protein N73, N169 Glycosite(s) Annotated Protein Sequence >tr|Q0P8C1|Q0P8C1_CAMJE Putative periplasmic protein OS=Campylobacter jejuni GN=Cj1496c PE=1 SV=1 MIKKFILLVFISSVVFGAEQDCEQYFEARKAQIELQTREFDEARQSLEAYKASFEALQKE RLENLEKKEAEVN*(73)ATLAKIEELKLENARLVEEQQKILNSINDKTQGRVKEIYSQMKDAAI ADVLSQMDAEDASKIMLSLESRKISGVLSKMDPKKASELTLLLKNLDNN*(169)ASN Sequence Around Glycosites (21 AA) ENLEKKEAEVNATLAKIEELK LTLLLKNLDNNASN Glycosite Sequence Logo seqlogo Glycosite Sequence Logo Technique(s) used for Glycosylation Detection SBA (soybean agglutinin) lectin-agarose affinity chromatography Technique(s) used for Glycosylated Residue(s) Detection Site-specific mutagenesis combined with Western blot analysis Protein Glycosylation- Implication Glycans are not directly involved in the function of Cj1496c. Glycan Information Glycan Annotation Linkage: Bac-Asn. 1406 Da heptasaccharide composed of GalNAc-α1,4-GalNAc-α1,4-[Glcβ1,3-]GalNAc-α1,4-GalNAc-α1,4-GalNAc-α1,3-Bac-β1,N-Asn-Xaa, where Bac is bacillosamine, 2,4-diacetamido-2,4,6-trideoxyglucopyranose. Technique(s) used for Glycan Identification 1H, 13C NMR spectroscopy- one dimensional TOCSY (total correlation spectroscopy) and NOESY (nuclear Overhauser effect spectroscopy); HMBC (heteronuclear multiple bond coherence), HMQC (heteronuclear multiple quantum correlation) spectra. Protein Glycosylation linked (PGL) gene(s) OST Gene Name pglB (Cj1126c) OST NCBI Gene ID 905417 OST GenBank Gene Sequence 905417 OST Protein Name PglB OST UniProtKB/ SwissProt ID Q0P9C8 OST NCBI RefSeq YP_002344519.1 OST EMBL-CDS CAL35243.1 OST UniProtKB Sequence >tr|Q0P9C8|Q0P9C8_CAMJE Oligosaccharide transferase to N-glycosylate proteins OS=Campylobacter jejuni GN=pglB PE=4 SV=1 MLKKEYLKNPYLVLFAMIVLAYVFSVFCRFYWVWWASEFNEYFFNNQLMIISNDGYAFAE GARDMIAGFHQPNDLSYYGSSLSTLTYWLYKITPFSFESIILYMSTFLSSLVVIPIILLA NEYKRPLMGFVAALLASVANSYYNRTMSGYYDTDMLVIVLPMFILFFMVRMILKKDFFSL IALPLFIGIYLWWYPSSYTLNVALIGLFLIYTLIFHRKEKIFYIAVILSSLTLSNIAWFY QSAIIVILFALFALEQKRLNFMIIGILGSATLIFLILSGGVDPILYQLKFYIFRSDESAN LTQGFMYFNVNQTIQEVENVDFSEFMRRISGSEIVFLFSLFGFVWLLRKHKSMIMALPIL VLGFLALKGGLRFTIYSVPVMALGFGFLLSEFKAILVKKYSQLTSNVCIVFATILTLAPV FIHIYNYKAPTVFSQNEASLLNQLKNIANREDYVVTWWDYGYPVRYYSDVKTLVDGGKHL GKDNFFPSFSLSKDEQAAANMARLSVEYTEKSFYAPQNDILKSDILQAMMKDYNQSNVDL FLASLSKPDFKIDTPKTRDIYLYMPARMSLIFSTVASFSFINLDTGVLDKPFTFSTAYPL DVKNGEIYLSNGVVLSDDFRSFKIGDNVVSVNSIVEINSIKQGEYKITPIDDKAQFYIFY LKDSAIPYAQFILMDKTMFNSAYVQMFFLGNYDKNLFDLVINSRDAKVFKLKI OST EC Number (BRENDA) 2.4.1.119 OST Genome Context Q0P9C8 Characterized Accessory Gene(s) PglA, PglJ, PglH, PglI, PglC are glycosyltransferases involved in the heptasaccharide assembly. PglFED are the bacillosamine biosynthetic enzymes. PglK is a flippase. PGL Additional Links CAZy Literatures Reference(s) 1) Maita, N., Nyirenda, J., Igura, M., Kamishikiryo, J. and Kohda, D. (2010) Comparative structural biology of eubacterial and archaeal oligosaccharyltransferases. J Biol Chem, 285, 4941-4950. [PubMed: 20007322] 2) Kakuda, T. and DiRita, V.J. (2006) Cj1496c encodes a Campylobacter jejuni glycoprotein that influences invasion of human epithelial cells and colonization of the chick gastrointestinal tract. Infect Immun, 74, 4715-4723. [PubMed: 16861659] 3) Olivier, N.B., Chen, M.M., Behr, Additional Comments Sequon feature: The consensus sequence for the C jejuni PglB is D/E-X'-N-X"-S/T (where X' and X" stand for any amino acid except proline) which is glycosylated when located in flexible, exposed structural elements. Tripeptide sequence NLT too has been found to be modified in vitro. Year of Identification 2002 Year of Validation 2006