ProGlyProt ID BC119 Organism Information Organism Name Campylobacter jejuni NCTC 11168 serotype O:2 Domain Bacteria Classification Family: Campylobacteraceae Order: Campylobacterales Class: Epsilonproteobacteria Division or phylum: "Proteobacteria" Taxonomic ID (NCBI) 197 Genome Sequence (s) GeneBank AL111168.1 EMBL AL111168 Gene Information Gene Name cgpA (Cj1670c) NCBI Gene ID 905945 GenBank Gene Sequence 905945 Protein Information Protein Name CgpA UniProtKB/SwissProt ID Q0P7V9 NCBI RefSeq YP_002345038.1 EMBL-CDS CAL35766.1 UniProtKB Sequence >tr|Q0P7V9|Q0P7V9_CAMJE Putative periplasmic protein OS=Campylobacter jejuni GN=cgpA PE=3 SV=1 MKTRILAIFFIFTSLLYADENPFKTDQNITLVAPPEFQKEEVKFNSSARILKSITFNYIN LDGSEDKIDLDVNKSIDWHDTYTISRFKSPDPSKVLDVSVTIPEKNSSKQESNSTANVEI PLQVAKIYDFISYAVYKNKIKLNTSDEMITDFSVGNPSKIVIDFRSKMISPTKNIRLSNS IFKRIDFGSHKGYYRLVIYLDGTYNYNIQKDATGYMINLL Sequence length 220 AA Subcellular Location Putative periplasmic Function Protein Structure PDB ID Glycosylation Status Glycosylation Type N (Asn) linked Experimentally Validated Glycosite(s) in Full Length Protein (Signal peptide: 1-18) N28, N106, N113 Experimentally Validated Glycosite(s ) in Mature Protein N10, N88, N95 Glycosite(s) Annotated Protein Sequence >tr|Q0P7V9|Q0P7V9_CAMJE Putative periplasmic protein OS=Campylobacter jejuni GN=cgpA PE=3 SV=1 MKTRILAIFFIFTSLLYADENPFKTDQN*(28)ITLVAPPEFQKEEVKFNSSARILKSITFNYIN LDGSEDKIDLDVNKSIDWHDTYTISRFKSPDPSKVLDVSVTIPEKN*(106)SSKQESN*(113)STANVEI PLQVAKIYDFISYAVYKNKIKLNTSDEMITDFSVGNPSKIVIDFRSKMISPTKNIRLSNS IFKRIDFGSHKGYYRLVIYLDGTYNYNIQKDATGYMINLL Sequence Around Glycosites (21 AA) ADENPFKTDQNITLVAPPEFQ LDVSVTIPEKNSSKQESNSTA PEKNSSKQESNSTANVEIPLQ Glycosite Sequence Logo seqlogo Glycosite Sequence Logo Technique(s) used for Glycosylation Detection SBA (soybean agglutinin) lectin-agarose affinity chromatography Technique(s) used for Glycosylated Residue(s) Detection CapLC-MS/MS (capillary liquid chromatography-tandem mass spectrometry) Protein Glycosylation- Implication Glycan Information Glycan Annotation Linkage: Bac-Asn. 1406 Da heptasaccharide composed of GalNAc-α1,4-GalNAc-α1,4-[Glcβ1,3-]GalNAc-α1,4-GalNAc-α1,4-GalNAc-α1,3-Bac-β1,N-Asn-Xaa, where Bac is bacillosamine, 2,4-diacetamido-2,4,6-trideoxyglucopyranose. Technique(s) used for Glycan Identification 1H, 13C NMR spectroscopy- one dimensional TOCSY (total correlation spectroscopy) and NOESY (nuclear Overhauser effect spectroscopy); HMBC (heteronuclear multiple bond coherence), HMQC (heteronuclear multiple quantum correlation) spectra. Protein Glycosylation linked (PGL) gene(s) OST Gene Name pglB (Cj1126c) OST NCBI Gene ID 905417 OST GenBank Gene Sequence 905417 OST Protein Name PglB OST UniProtKB/ SwissProt ID Q0P9C8 OST NCBI RefSeq YP_002344519.1 OST EMBL-CDS CAL35243.1 OST UniProtKB Sequence >tr|Q0P9C8|Q0P9C8_CAMJE Oligosaccharide transferase to N-glycosylate proteins OS=Campylobacter jejuni GN=pglB PE=4 SV=1 MLKKEYLKNPYLVLFAMIVLAYVFSVFCRFYWVWWASEFNEYFFNNQLMIISNDGYAFAE GARDMIAGFHQPNDLSYYGSSLSTLTYWLYKITPFSFESIILYMSTFLSSLVVIPIILLA NEYKRPLMGFVAALLASVANSYYNRTMSGYYDTDMLVIVLPMFILFFMVRMILKKDFFSL IALPLFIGIYLWWYPSSYTLNVALIGLFLIYTLIFHRKEKIFYIAVILSSLTLSNIAWFY QSAIIVILFALFALEQKRLNFMIIGILGSATLIFLILSGGVDPILYQLKFYIFRSDESAN LTQGFMYFNVNQTIQEVENVDFSEFMRRISGSEIVFLFSLFGFVWLLRKHKSMIMALPIL VLGFLALKGGLRFTIYSVPVMALGFGFLLSEFKAILVKKYSQLTSNVCIVFATILTLAPV FIHIYNYKAPTVFSQNEASLLNQLKNIANREDYVVTWWDYGYPVRYYSDVKTLVDGGKHL GKDNFFPSFSLSKDEQAAANMARLSVEYTEKSFYAPQNDILKSDILQAMMKDYNQSNVDL FLASLSKPDFKIDTPKTRDIYLYMPARMSLIFSTVASFSFINLDTGVLDKPFTFSTAYPL DVKNGEIYLSNGVVLSDDFRSFKIGDNVVSVNSIVEINSIKQGEYKITPIDDKAQFYIFY LKDSAIPYAQFILMDKTMFNSAYVQMFFLGNYDKNLFDLVINSRDAKVFKLKI OST EC Number (BRENDA) 2.4.1.119 OST Genome Context Q0P9C8 Characterized Accessory Gene(s) PglA, PglJ, PglH, PglI, PglC are glycosyltransferases involved in the heptasaccharide assembly. PglFED are the bacillosamine biosynthetic enzymes. PglK is a flippase. PGL Additional Links CAZy Literatures Reference(s) 1) Maita, N., Nyirenda, J., Igura, M., Kamishikiryo, J. and Kohda, D. (2010) Comparative structural biology of eubacterial and archaeal oligosaccharyltransferases. J Biol Chem, 285, 4941-4950. [PubMed: 20007322] 2) Olivier, N.B., Chen, M.M., Behr, J.R. and Imperiali, B. (2006) In vitro biosynthesis of UDP-N,N'-diacetylbacillosamine by enzymes of the Campylobacter jejuni general protein glycosylation system. Biochemistry, 45, 13659-13669. [PubMed: 17087520] 3) Linton, D., Allan, E., Karl Additional Comments Sequon feature: The consensus sequence for the C jejuni PglB is D/E-X'-N-X"-S/T (where X' and X" stand for any amino acid except proline) which is glycosylated when located in flexible, exposed structural elements. Tripeptide sequence NLT too has been found to be modified in vitro. Year of Identification 2002 Year of Validation 2002