ProGlyProt ID BC118 Organism Information Organism Name Campylobacter jejuni NCTC 11168 serotype O:2 Domain Bacteria Classification Family: Campylobacteraceae Order: Campylobacterales Class: Epsilonproteobacteria Division or phylum: "Proteobacteria" Taxonomic ID (NCBI) 197 Genome Sequence (s) GeneBank AL111168.1 EMBL AL111168 Gene Information Gene Name Cj0367c (acrA/cmeA) NCBI Gene ID 904690 GenBank Gene Sequence 904690 Protein Information Protein Name AcrA or CmeA UniProtKB/SwissProt ID Q0PBE3 NCBI RefSeq YP_002343804.1 EMBL-CDS CAL34517.1 UniProtKB Sequence >tr|Q0PBE3|Q0PBE3_CAMJE Periplasmic fusion protein CmeA OS=Campylobacter jejuni GN=cmeA PE=4 SV=1 MKLFQKNTILALGVVLLLTACSKEEAPKIQMPPQPVTTMSAKSEDLPLSFTYPAKLVSDY DVIIKPQVSGVIENKLFKAGDKVKKGQTLFIIEQDKFKASVDSAYGQALMAKATFENASK DFNRSKALFSKSAISQKEYDSSLATFNNSKASLASARAQLANARIDLDHTEIKAPFDGTI GDALVNIGDYVSASTTELVRVTNLNPIYADFFISDTDKLNLVRNTQNGKWDLDSIHANLN LNGETVQGKLYFIDSVIDANSGTVKAKAIFDNNNSTLLPGAFATITSEGFIQKNGFKVPQ IAVKQNQNDVYVLLVKNGKVEKSSVHISYQNNEYAIIDKGLQNGDKIILDNFKKIQVGSE VKEIGAQ Sequence length 367 AA Subcellular Location Periplasm Function It is a fusion lipoprtein involved in antibiotic resistance. Part of the CmeABC multidrug efflux system. Protein Structure PDB ID Glycosylation Status Glycosylation Type N (Asn) linked Experimentally Validated Glycosite(s) in Full Length Protein N123, N273 Experimentally Validated Glycosite(s ) in Mature Protein N123, N273 Glycosite(s) Annotated Protein Sequence >tr|Q0PBE3|Q0PBE3_CAMJE Periplasmic fusion protein CmeA OS=Campylobacter jejuni GN=cmeA PE=4 SV=1 MKLFQKNTILALGVVLLLTACSKEEAPKIQMPPQPVTTMSAKSEDLPLSFTYPAKLVSDY DVIIKPQVSGVIENKLFKAGDKVKKGQTLFIIEQDKFKASVDSAYGQALMAKATFENASK DFN*(123)RSKALFSKSAISQKEYDSSLATFNNSKASLASARAQLANARIDLDHTEIKAPFDGTI GDALVNIGDYVSASTTELVRVTNLNPIYADFFISDTDKLNLVRNTQNGKWDLDSIHANLN LNGETVQGKLYFIDSVIDANSGTVKAKAIFDNN*(273)NSTLLPGAFATITSEGFIQKNGFKVPQ IAVKQNQNDVYVLLVKNGKVEKSSVHISYQNNEYAIIDKGLQNGDKIILDNFKKIQVGSE VKEIGAQ Sequence Around Glycosites (21 AA) ATFENASKDFNRSKALFSKSA TVKAKAIFDNNNSTLLPGAFA Glycosite Sequence Logo seqlogo Glycosite Sequence Logo Technique(s) used for Glycosylation Detection Immunoblot analysis using glycosylation-specific R12 antiserum Technique(s) used for Glycosylated Residue(s) Detection Site-directed mutagenesis Protein Glycosylation- Implication Glycan Information Glycan Annotation Linkage: Bac-Asn. 1406 Da heptasaccharide composed of GalNAc-α1,4-GalNAc-α1,4-[Glcβ1,3-]GalNAc-α1,4-GalNAc-α1,4-GalNAc-α1,3-Bac-β1,N-Asn-Xaa, where Bac is bacillosamine, 2,4-diacetamido-2,4,6-trideoxyglucopyranose. Technique(s) used for Glycan Identification 1H, 13C NMR spectroscopy- one dimensional TOCSY (total correlation spectroscopy) and NOESY (nuclear Overhauser effect spectroscopy); HMBC (heteronuclear multiple bond coherence), HMQC (heteronuclear multiple quantum correlation) spectra. Protein Glycosylation linked (PGL) gene(s) OST Gene Name pglB (Cj1126c) OST NCBI Gene ID 905417 OST GenBank Gene Sequence 905417 OST Protein Name PglB OST UniProtKB/ SwissProt ID Q0P9C8 OST NCBI RefSeq YP_002344519.1 OST EMBL-CDS CAL35243.1 OST UniProtKB Sequence >tr|Q0P9C8|Q0P9C8_CAMJE Oligosaccharide transferase to N-glycosylate proteins OS=Campylobacter jejuni GN=pglB PE=4 SV=1 MLKKEYLKNPYLVLFAMIVLAYVFSVFCRFYWVWWASEFNEYFFNNQLMIISNDGYAFAE GARDMIAGFHQPNDLSYYGSSLSTLTYWLYKITPFSFESIILYMSTFLSSLVVIPIILLA NEYKRPLMGFVAALLASVANSYYNRTMSGYYDTDMLVIVLPMFILFFMVRMILKKDFFSL IALPLFIGIYLWWYPSSYTLNVALIGLFLIYTLIFHRKEKIFYIAVILSSLTLSNIAWFY QSAIIVILFALFALEQKRLNFMIIGILGSATLIFLILSGGVDPILYQLKFYIFRSDESAN LTQGFMYFNVNQTIQEVENVDFSEFMRRISGSEIVFLFSLFGFVWLLRKHKSMIMALPIL VLGFLALKGGLRFTIYSVPVMALGFGFLLSEFKAILVKKYSQLTSNVCIVFATILTLAPV FIHIYNYKAPTVFSQNEASLLNQLKNIANREDYVVTWWDYGYPVRYYSDVKTLVDGGKHL GKDNFFPSFSLSKDEQAAANMARLSVEYTEKSFYAPQNDILKSDILQAMMKDYNQSNVDL FLASLSKPDFKIDTPKTRDIYLYMPARMSLIFSTVASFSFINLDTGVLDKPFTFSTAYPL DVKNGEIYLSNGVVLSDDFRSFKIGDNVVSVNSIVEINSIKQGEYKITPIDDKAQFYIFY LKDSAIPYAQFILMDKTMFNSAYVQMFFLGNYDKNLFDLVINSRDAKVFKLKI OST EC Number (BRENDA) 2.4.1.119 OST Genome Context Q0P9C8 Characterized Accessory Gene(s) PglA, PglJ, PglH, PglI, PglC are glycosyltransferases involved in the heptasaccharide assembly. PglFED are the bacillosamine biosynthetic enzymes. PglK is a flippase. PGL Additional Links CAZy Literatures Reference(s) 1) Maita, N., Nyirenda, J., Igura, M., Kamishikiryo, J. and Kohda, D. (2010) Comparative structural biology of eubacterial and archaeal oligosaccharyltransferases. J Biol Chem, 285, 4941-4950. [PubMed: 20007322] 2) Kowarik, M., Young, N.M., Numao, S., Schulz, B.L., Hug, I., Callewaert, N., Mills, D.C., Watson, D.C., Hernandez, M., Kelly, J.F. et al. (2006) Definition of the bacterial N-glycosylation site consensus sequence. EMBO J, 25, 1957-1966.[PubMed: 16619027] 3) Olivier, N.B., Che Additional Comments AcrA is a native glycoprotein. However, its glycosylation was characterized in E. coli in which C. jejuni glycosylation machinery had been reconstituted. Sequon feature: The consensus sequence for the C jejuni PglB is D/E-X'-N-X"-S/T (where X' and X" stand for any amino acid except proline) which is glycosylated when located in flexible, exposed structural elements. Tripeptide sequence NLT too has been found to be modified in vitro. Year of Identification 2002 Year of Validation 2002