| ProGlyProt ID | BC117 |
| Organism Information |
| Organism Name | Campylobacter jejuni NCTC 11168 |
| Domain | Bacteria |
| Classification | Family: Campylobacteraceae
Order: Campylobacterales
Class: Epsilonproteobacteria
Division or phylum: "Proteobacteria" |
| Taxonomic ID (NCBI) | 197 |
| Genome Sequence (s) |
| EMBL | |
| Gene Information |
| Gene Name | |
| NCBI Gene ID | |
| Protein Information |
| Protein Name | Synthetic Glycopeptide |
| UniProtKB/SwissProt ID | Not Applicable |
| NCBI RefSeq | |
| EMBL-CDS | |
| UniProtKB Sequence | KDFNVSKA |
| Sequence length | 5 AA |
| Subcellular Location | NA |
| Function | NA |
| Protein Structure |
| PDB ID | |
| Glycosylation Status |
| Glycosylation Type | N (Asn) linked |
| Experimentally Validated Glycosite(s) in Full Length Protein | N4 |
| Experimentally Validated Glycosite(s ) in Mature Protein | N4 |
| Glycosite(s) Annotated Protein Sequence | KDFN*(4)VSKA |
| Sequence Around Glycosites (21 AA) | |
| Glycosite Sequence Logo | seqlogo |
| Glycosite Sequence Logo |  |
| Technique(s) used for Glycosylation Detection | In vitro chemo enzymatic glycosylation,HPLC analysis of glycopeptides with reference to known glycopeptide HPLC profiles |
| Technique(s) used for Glycosylated Residue(s) Detection | Not applicable |
| Protein Glycosylation- Implication | Not applicable |
| Glycan Information |
| Glycan Annotation | GalNAc-α1,3-bacillosamine. |
| Technique(s) used for Glycan Identification | |
| Protein Glycosylation linked (PGL) gene(s) |
| OST Gene Name | pglB |
| OST NCBI Gene ID | 4682880 |
| OST GenBank Gene Sequence | 4682880 |
| OST Protein Name | PglB |
| OST UniProtKB/ SwissProt ID | A1W0B2 |
| OST NCBI RefSeq | YP_001000803.1 |
| OST EMBL-CDS | EAQ72203.1 |
| OST UniProtKB Sequence | >tr|A1W0B2|A1W0B2_CAMJJ General glycosylation pathway protein OS=Campylobacter jejuni subsp. jejuni serotype O:23/36 (strain 81-176) GN=pglB PE=4 SV=1
MLKKEYLKNPYLVLFAMIVLAYVFSVLCRFYWIWWASEFNEYFFNNQLMIISNDGYAFAE
GARDMIAGFHQPNDLSYYGSSLSTLTYWLYKITPFSFESIILYMSTFLSSLVVIPIILLA
NEYKRPLMGFVAALLASIANSYYNRTMSGYYDTDMLVIVLPMFILFFMVRMILKKDFFSL
IALPLFIGIYLWWYPSSYTLNVALIGLFLIYTLIFHRKEKIFYIAVILSSLTLSNIAWFY
QSTIIVILFALFALEQKRLNFVIIGILASVTLIFLILSGGVDPILYQLKFYIFRSDESAN
LTQGFMYFNVNQTIQEVENVDLSEFMRRISGSEIVFLFSLFGFVWLLRKHKSMIMALPIL
VLGFLALKGGLRFTIYSVPVMALGFGFLLSEFKAILVKKYSQLTSNVCIVFATILTLAPV
FIHIYNYKAPTVFSQNEASLLNQLKNIANREDYVVTWWDYGYPVRYYSDVKTLVDGGKHL
GKDNFFPSFALSKDEQAAANMARLSVEYTEKSFYAPQNDILKTDILQAMMKDYNQSNVDL
FLASLSKPDFKIDTPKTRDIYLYMPARMSLIFSTVASFSFINLDTGVLDKPFTFSTAYPL
DVKNGEIYLSNGVVLSDDFRSFKIGDNVVSVNSIVEINSIKQGEYKITPIDDKAQFYIFY
LKDSAIPYAQFILMDKTMFNSAYVQMFFLGNYDKNLFDLVINSRDAKVFKLKI
|
| OST EC Number (BRENDA) | 2.4.1.119 |
| OST Genome Context | A1W0B2 |
| Characterized Accessory Gene(s) | |
| PGL Additional Links | CAZy |
| Literatures |
| Reference(s) | 1) Glover, K.J., Weerapana, E., Numao, S. and Imperiali, B. (2005) Chemoenzymatic synthesis of glycopeptides with PglB, a bacterial oligosaccharyl transferase from Campylobacter jejuni. Chem Biol, 12, 1311-1315. [PubMed: 16356848] |
| Additional Comments | Engineered glycopeptide.
In vitro characterization of the oligosaccharyl transferase activity of PglB protein from membrane fraction of Campylobacter jejuni using a synthetic disaccharide glycan donor (GalNAc-α1,3-bacillosamine-pyrophosphate-undecaprenyl) and a peptide acceptor substrate (KDFNVSKA). |
| Year of Identification | 2005 |
| Year of Validation | 2005 |
