ProGlyProt IDBC117
Organism Information
Organism NameCampylobacter jejuni NCTC 11168
DomainBacteria
ClassificationFamily: Campylobacteraceae
Order: Campylobacterales
Class: Epsilonproteobacteria
Division or phylum: "Proteobacteria"
Taxonomic ID (NCBI)197
Genome Sequence (s)
EMBL
Gene Information
Gene Name
NCBI Gene ID
Protein Information
Protein NameSynthetic Glycopeptide
UniProtKB/SwissProt IDNot Applicable
NCBI RefSeq
EMBL-CDS
UniProtKB SequenceKDFNVSKA
Sequence length5 AA
Subcellular LocationNA
FunctionNA
Protein Structure
PDB ID
Glycosylation Status
Glycosylation TypeN (Asn) linked
Experimentally Validated Glycosite(s) in Full Length ProteinN4
Experimentally Validated Glycosite(s ) in Mature ProteinN4
Glycosite(s) Annotated Protein SequenceKDFN*(4)VSKA
Sequence Around Glycosites (21 AA)
Glycosite Sequence Logoseqlogo
Glycosite Sequence Logo
Technique(s) used for Glycosylation DetectionIn vitro chemo enzymatic glycosylation,HPLC analysis of glycopeptides with reference to known glycopeptide HPLC profiles
Technique(s) used for Glycosylated Residue(s) DetectionNot applicable
Protein Glycosylation- ImplicationNot applicable
Glycan Information
Glycan AnnotationGalNAc-α1,3-bacillosamine.
Technique(s) used for Glycan Identification
Protein Glycosylation linked (PGL) gene(s)
OST Gene NamepglB
OST NCBI Gene ID4682880
OST GenBank Gene Sequence4682880
OST Protein NamePglB
OST UniProtKB/ SwissProt IDA1W0B2
OST NCBI RefSeqYP_001000803.1
OST EMBL-CDSEAQ72203.1
OST UniProtKB Sequence>tr|A1W0B2|A1W0B2_CAMJJ General glycosylation pathway protein OS=Campylobacter jejuni subsp. jejuni serotype O:23/36 (strain 81-176) GN=pglB PE=4 SV=1 MLKKEYLKNPYLVLFAMIVLAYVFSVLCRFYWIWWASEFNEYFFNNQLMIISNDGYAFAE GARDMIAGFHQPNDLSYYGSSLSTLTYWLYKITPFSFESIILYMSTFLSSLVVIPIILLA NEYKRPLMGFVAALLASIANSYYNRTMSGYYDTDMLVIVLPMFILFFMVRMILKKDFFSL IALPLFIGIYLWWYPSSYTLNVALIGLFLIYTLIFHRKEKIFYIAVILSSLTLSNIAWFY QSTIIVILFALFALEQKRLNFVIIGILASVTLIFLILSGGVDPILYQLKFYIFRSDESAN LTQGFMYFNVNQTIQEVENVDLSEFMRRISGSEIVFLFSLFGFVWLLRKHKSMIMALPIL VLGFLALKGGLRFTIYSVPVMALGFGFLLSEFKAILVKKYSQLTSNVCIVFATILTLAPV FIHIYNYKAPTVFSQNEASLLNQLKNIANREDYVVTWWDYGYPVRYYSDVKTLVDGGKHL GKDNFFPSFALSKDEQAAANMARLSVEYTEKSFYAPQNDILKTDILQAMMKDYNQSNVDL FLASLSKPDFKIDTPKTRDIYLYMPARMSLIFSTVASFSFINLDTGVLDKPFTFSTAYPL DVKNGEIYLSNGVVLSDDFRSFKIGDNVVSVNSIVEINSIKQGEYKITPIDDKAQFYIFY LKDSAIPYAQFILMDKTMFNSAYVQMFFLGNYDKNLFDLVINSRDAKVFKLKI
OST EC Number (BRENDA)2.4.1.119
OST Genome ContextA1W0B2
Characterized Accessory Gene(s)
PGL Additional LinksCAZy
Literatures
Reference(s)1) Glover, K.J., Weerapana, E., Numao, S. and Imperiali, B. (2005) Chemoenzymatic synthesis of glycopeptides with PglB, a bacterial oligosaccharyl transferase from Campylobacter jejuni. Chem Biol, 12, 1311-1315. [PubMed: 16356848]
Additional CommentsEngineered glycopeptide.
In vitro characterization of the oligosaccharyl transferase activity of PglB protein from membrane fraction of Campylobacter jejuni using a synthetic disaccharide glycan donor (GalNAc-α1,3-bacillosamine-pyrophosphate-undecaprenyl) and a peptide acceptor substrate (KDFNVSKA).
Year of Identification2005
Year of Validation2005