| ProGlyProt ID | BC116 |
| Organism Information |
| Organism Name | Campylobacter jejuni serotype O:23/36 (strain 81-176) |
| Domain | Bacteria |
| Classification | Family: Campylobacteraceae
Order: Campylobacterales
Class: Epsilonproteobacteria
Division or phylum: "Proteobacteria" |
| Taxonomic ID (NCBI) | 354242 |
| Genome Sequence (s) |
| GeneBank | CP000550.1 |
| EMBL | CP000550 |
| Gene Information |
| Gene Name | Cjp3 (CJJ81176_pVir0003) |
| NCBI Gene ID | 1343085 |
| GenBank Gene Sequence | 1343085 |
| Protein Information |
| Protein Name | VirB10 protein |
| UniProtKB/SwissProt ID | Q9KIS0 |
| NCBI RefSeq | NP_863300.1 |
| EMBL-CDS | EAQ71778.1 |
| UniProtKB Sequence | >tr|Q9KIS0|Q9KIS0_CAMJJ VirB10 OS=Campylobacter jejuni subsp. jejuni serotype O:23/36 (strain 81-176) GN=virB10 PE=4 SV=1
MKKSFLSLSLATILLSNNLFAEDIFDQTSEENVSKNISKKDNQSQNLLNKEDLDYLFKNS
DTKFPVTDYIYKGGFKEPNEDIKIHSEEKPKKEEDNNITKLAKIEEKKQELIQKNQQIAK
EIHQDNISSQERKIKKLIRDSILADRGNTQIYFQENSSKYGVDGFSNQKSIDVSTNEHRL
YRMIRAGRLIPALLTSAISSDLQGIVTAQIEQDIYASMGNAVLIPRGSKAIGFYKNDNKI
GQNRLEIQWREIITPQGVNILLTNALASDNMGMAGAVGSVNNKYLERYGMPYALSTISNV
LLLSIASKAGNNNYAQEVYSQSKNDVSTIVDDIIQQQSQIKPTIEIKSGSRIFIVPTNHM
WFAKPKNNEVLIQYFQDN
|
| Sequence length | 378 AA |
| Subcellular Location | Periplasmic or on surface |
| Function | Type IV secretion (TFSS) homolog. The TFSS genes present on pVir have been proposed to encode proteins that form a functional secretion channel that appears to affect both intestinal epithelial cell invasion and natural competence. |
| Protein Structure |
| PDB ID | |
| Glycosylation Status |
| Glycosylation Type | N (Asn) linked |
| Experimentally Validated Glycosite(s) in Full Length Protein | N32, N97 |
| Experimentally Validated Glycosite(s ) in Mature Protein | N32, N97 |
| Glycosite(s) Annotated Protein Sequence | >tr|Q9KIS0|Q9KIS0_CAMJJ VirB10 OS=Campylobacter jejuni subsp. jejuni serotype O:23/36 (strain 81-176) GN=virB10 PE=4 SV=1
MKKSFLSLSLATILLSNNLFAEDIFDQTSEEN*(32)VSKNISKKDNQSQNLLNKEDLDYLFKNS
DTKFPVTDYIYKGGFKEPNEDIKIHSEEKPKKEEDNN*(97)ITKLAKIEEKKQELIQKNQQIAK
EIHQDNISSQERKIKKLIRDSILADRGNTQIYFQENSSKYGVDGFSNQKSIDVSTNEHRL
YRMIRAGRLIPALLTSAISSDLQGIVTAQIEQDIYASMGNAVLIPRGSKAIGFYKNDNKI
GQNRLEIQWREIITPQGVNILLTNALASDNMGMAGAVGSVNNKYLERYGMPYALSTISNV
LLLSIASKAGNNNYAQEVYSQSKNDVSTIVDDIIQQQSQIKPTIEIKSGSRIFIVPTNHM
WFAKPKNNEVLIQYFQDN
|
| Sequence Around Glycosites (21 AA) | EDIFDQTSEENVSKNISKKDN
EEKPKKEEDNNITKLAKIEEK
|
| Glycosite Sequence Logo | seqlogo |
| Glycosite Sequence Logo |  |
| Technique(s) used for Glycosylation Detection | Mass shift detected on SDS-polyacrylamide gel and SBA (soybean agglutinin) lectin-agarose affinity chromatography |
| Technique(s) used for Glycosylated Residue(s) Detection | Site-directed mutagenesis |
| Protein Glycosylation- Implication | Glycosylation of VirB10 at N97, but not at N32, is essential for wild-type (full) levels of competence. VirB10 is not detected in the pgl mutants. It reflects instability of the nonglycosylated protein, perhaps a result of an inability to interact with other TFSS proteins. |
| Glycan Information |
| Glycan Annotation | Terminal α1,3-linked GalNAc. |
| Technique(s) used for Glycan Identification | |
| Protein Glycosylation linked (PGL) gene(s) |
| OST Gene Name | pglB |
| OST NCBI Gene ID | 4682880 |
| OST GenBank Gene Sequence | 4682880 |
| OST Protein Name | PglB |
| OST UniProtKB/ SwissProt ID | A1W0B2 |
| OST NCBI RefSeq | YP_001000803.1 |
| OST EMBL-CDS | EAQ72203.1 |
| OST UniProtKB Sequence | >tr|A1W0B2|A1W0B2_CAMJJ General glycosylation pathway protein OS=Campylobacter jejuni subsp. jejuni serotype O:23/36 (strain 81-176) GN=pglB PE=4 SV=1
MLKKEYLKNPYLVLFAMIVLAYVFSVLCRFYWIWWASEFNEYFFNNQLMIISNDGYAFAE
GARDMIAGFHQPNDLSYYGSSLSTLTYWLYKITPFSFESIILYMSTFLSSLVVIPIILLA
NEYKRPLMGFVAALLASIANSYYNRTMSGYYDTDMLVIVLPMFILFFMVRMILKKDFFSL
IALPLFIGIYLWWYPSSYTLNVALIGLFLIYTLIFHRKEKIFYIAVILSSLTLSNIAWFY
QSTIIVILFALFALEQKRLNFVIIGILASVTLIFLILSGGVDPILYQLKFYIFRSDESAN
LTQGFMYFNVNQTIQEVENVDLSEFMRRISGSEIVFLFSLFGFVWLLRKHKSMIMALPIL
VLGFLALKGGLRFTIYSVPVMALGFGFLLSEFKAILVKKYSQLTSNVCIVFATILTLAPV
FIHIYNYKAPTVFSQNEASLLNQLKNIANREDYVVTWWDYGYPVRYYSDVKTLVDGGKHL
GKDNFFPSFALSKDEQAAANMARLSVEYTEKSFYAPQNDILKTDILQAMMKDYNQSNVDL
FLASLSKPDFKIDTPKTRDIYLYMPARMSLIFSTVASFSFINLDTGVLDKPFTFSTAYPL
DVKNGEIYLSNGVVLSDDFRSFKIGDNVVSVNSIVEINSIKQGEYKITPIDDKAQFYIFY
LKDSAIPYAQFILMDKTMFNSAYVQMFFLGNYDKNLFDLVINSRDAKVFKLKI
|
| OST EC Number (BRENDA) | 2.4.1.119 |
| OST Genome Context | A1W0B2 |
| Characterized Accessory Gene(s) | |
| PGL Additional Links | CAZy |
| Literatures |
| Reference(s) | 1) Maita, N., Nyirenda, J., Igura, M., Kamishikiryo, J. and Kohda, D. (2010) Comparative structural biology of eubacterial and archaeal oligosaccharyltransferases. J Biol Chem, 285, 4941-4950. [PubMed: 20007322]
2) Larsen, J.C., Szymanski, C. and Guerry, P. (2004) N-linked protein glycosylation is required for full competence in Campylobacter jejuni 81-176. J Bacteriol, 186, 6508-6514. [PubMed: 15375132] |
| Additional Comments | Sequon feature: The consensus sequence for the C jejuni PglB is D/E-X'-N-X"-S/T (where X' and X" stand for any amino acid except proline). |
| Year of Identification | 2004 |
| Year of Validation | 2004 |
