ProGlyProt ID BC116 Organism Information Organism Name Campylobacter jejuni serotype O:23/36 (strain 81-176) Domain Bacteria Classification Family: Campylobacteraceae Order: Campylobacterales Class: Epsilonproteobacteria Division or phylum: "Proteobacteria" Taxonomic ID (NCBI) 354242 Genome Sequence (s) GeneBank CP000550.1 EMBL CP000550 Gene Information Gene Name Cjp3 (CJJ81176_pVir0003) NCBI Gene ID 1343085 GenBank Gene Sequence 1343085 Protein Information Protein Name VirB10 protein UniProtKB/SwissProt ID Q9KIS0 NCBI RefSeq NP_863300.1 EMBL-CDS EAQ71778.1 UniProtKB Sequence >tr|Q9KIS0|Q9KIS0_CAMJJ VirB10 OS=Campylobacter jejuni subsp. jejuni serotype O:23/36 (strain 81-176) GN=virB10 PE=4 SV=1 MKKSFLSLSLATILLSNNLFAEDIFDQTSEENVSKNISKKDNQSQNLLNKEDLDYLFKNS DTKFPVTDYIYKGGFKEPNEDIKIHSEEKPKKEEDNNITKLAKIEEKKQELIQKNQQIAK EIHQDNISSQERKIKKLIRDSILADRGNTQIYFQENSSKYGVDGFSNQKSIDVSTNEHRL YRMIRAGRLIPALLTSAISSDLQGIVTAQIEQDIYASMGNAVLIPRGSKAIGFYKNDNKI GQNRLEIQWREIITPQGVNILLTNALASDNMGMAGAVGSVNNKYLERYGMPYALSTISNV LLLSIASKAGNNNYAQEVYSQSKNDVSTIVDDIIQQQSQIKPTIEIKSGSRIFIVPTNHM WFAKPKNNEVLIQYFQDN Sequence length 378 AA Subcellular Location Periplasmic or on surface Function Type IV secretion (TFSS) homolog. The TFSS genes present on pVir have been proposed to encode proteins that form a functional secretion channel that appears to affect both intestinal epithelial cell invasion and natural competence. Protein Structure PDB ID Glycosylation Status Glycosylation Type N (Asn) linked Experimentally Validated Glycosite(s) in Full Length Protein N32, N97 Experimentally Validated Glycosite(s ) in Mature Protein N32, N97 Glycosite(s) Annotated Protein Sequence >tr|Q9KIS0|Q9KIS0_CAMJJ VirB10 OS=Campylobacter jejuni subsp. jejuni serotype O:23/36 (strain 81-176) GN=virB10 PE=4 SV=1 MKKSFLSLSLATILLSNNLFAEDIFDQTSEEN*(32)VSKNISKKDNQSQNLLNKEDLDYLFKNS DTKFPVTDYIYKGGFKEPNEDIKIHSEEKPKKEEDNN*(97)ITKLAKIEEKKQELIQKNQQIAK EIHQDNISSQERKIKKLIRDSILADRGNTQIYFQENSSKYGVDGFSNQKSIDVSTNEHRL YRMIRAGRLIPALLTSAISSDLQGIVTAQIEQDIYASMGNAVLIPRGSKAIGFYKNDNKI GQNRLEIQWREIITPQGVNILLTNALASDNMGMAGAVGSVNNKYLERYGMPYALSTISNV LLLSIASKAGNNNYAQEVYSQSKNDVSTIVDDIIQQQSQIKPTIEIKSGSRIFIVPTNHM WFAKPKNNEVLIQYFQDN Sequence Around Glycosites (21 AA) EDIFDQTSEENVSKNISKKDN EEKPKKEEDNNITKLAKIEEK Glycosite Sequence Logo seqlogo Glycosite Sequence Logo Technique(s) used for Glycosylation Detection Mass shift detected on SDS-polyacrylamide gel and SBA (soybean agglutinin) lectin-agarose affinity chromatography Technique(s) used for Glycosylated Residue(s) Detection Site-directed mutagenesis Protein Glycosylation- Implication Glycosylation of VirB10 at N97, but not at N32, is essential for wild-type (full) levels of competence. VirB10 is not detected in the pgl mutants. It reflects instability of the nonglycosylated protein, perhaps a result of an inability to interact with other TFSS proteins. Glycan Information Glycan Annotation Terminal α1,3-linked GalNAc. Technique(s) used for Glycan Identification Protein Glycosylation linked (PGL) gene(s) OST Gene Name pglB OST NCBI Gene ID 4682880 OST GenBank Gene Sequence 4682880 OST Protein Name PglB OST UniProtKB/ SwissProt ID A1W0B2 OST NCBI RefSeq YP_001000803.1 OST EMBL-CDS EAQ72203.1 OST UniProtKB Sequence >tr|A1W0B2|A1W0B2_CAMJJ General glycosylation pathway protein OS=Campylobacter jejuni subsp. jejuni serotype O:23/36 (strain 81-176) GN=pglB PE=4 SV=1 MLKKEYLKNPYLVLFAMIVLAYVFSVLCRFYWIWWASEFNEYFFNNQLMIISNDGYAFAE GARDMIAGFHQPNDLSYYGSSLSTLTYWLYKITPFSFESIILYMSTFLSSLVVIPIILLA NEYKRPLMGFVAALLASIANSYYNRTMSGYYDTDMLVIVLPMFILFFMVRMILKKDFFSL IALPLFIGIYLWWYPSSYTLNVALIGLFLIYTLIFHRKEKIFYIAVILSSLTLSNIAWFY QSTIIVILFALFALEQKRLNFVIIGILASVTLIFLILSGGVDPILYQLKFYIFRSDESAN LTQGFMYFNVNQTIQEVENVDLSEFMRRISGSEIVFLFSLFGFVWLLRKHKSMIMALPIL VLGFLALKGGLRFTIYSVPVMALGFGFLLSEFKAILVKKYSQLTSNVCIVFATILTLAPV FIHIYNYKAPTVFSQNEASLLNQLKNIANREDYVVTWWDYGYPVRYYSDVKTLVDGGKHL GKDNFFPSFALSKDEQAAANMARLSVEYTEKSFYAPQNDILKTDILQAMMKDYNQSNVDL FLASLSKPDFKIDTPKTRDIYLYMPARMSLIFSTVASFSFINLDTGVLDKPFTFSTAYPL DVKNGEIYLSNGVVLSDDFRSFKIGDNVVSVNSIVEINSIKQGEYKITPIDDKAQFYIFY LKDSAIPYAQFILMDKTMFNSAYVQMFFLGNYDKNLFDLVINSRDAKVFKLKI OST EC Number (BRENDA) 2.4.1.119 OST Genome Context A1W0B2 Characterized Accessory Gene(s) PGL Additional Links CAZy Literatures Reference(s) 1) Maita, N., Nyirenda, J., Igura, M., Kamishikiryo, J. and Kohda, D. (2010) Comparative structural biology of eubacterial and archaeal oligosaccharyltransferases. J Biol Chem, 285, 4941-4950. [PubMed: 20007322] 2) Larsen, J.C., Szymanski, C. and Guerry, P. (2004) N-linked protein glycosylation is required for full competence in Campylobacter jejuni 81-176. J Bacteriol, 186, 6508-6514. [PubMed: 15375132] Additional Comments Sequon feature: The consensus sequence for the C jejuni PglB is D/E-X'-N-X"-S/T (where X' and X" stand for any amino acid except proline). Year of Identification 2004 Year of Validation 2004