ProGlyProt IDBC109
Organism Information
Organism NameBacteroides fragilis (strain ATCC 25285 / NCTC 9343)
DomainBacteria
ClassificationFamily: Bacteroidaceae
Order: "Bacteroidales"
Class: "Bacteroidia"
Division or phylum: "Bacteroidetes"
Taxonomic ID (NCBI)272559
Genome Sequence (s)
GeneBankCR626927.1
EMBLCR626927
Gene Information
Gene NameBF2494
NCBI Gene ID3287481
GenBank Gene Sequence3287481
Protein Information
Protein NameBF2494
UniProtKB/SwissProt IDQ5LCH1
NCBI RefSeqYP_212118.1
EMBL-CDSCAH08194.1
UniProtKB Sequence>tr|Q5LCH1|Q5LCH1_BACFN Putative uncharacterized protein OS=Bacteroides fragilis (strain ATCC 25285 / NCTC 9343) GN=BF2494 PE=4 SV=1 MVLLMAVSFAFAQEKNVKEAKSIAGEVKPDFAKAEQLINEALTNPETKDNAATWDVAGYI QKRINEKEMENAYLRKPYDTLKVYNSVLNMYNYYVKCDELAQIPNEKGKIKNKYRSANSK TILAERPNLINGGIQYFNLNKNEDALKYFAAYVDAATLPMMEKENLLEKDTILPQVAYYA TLAADRVGDKDAVMKYAQYALKDKENGQFAMQLLTDAYKAKGDTAKWVEKLQEGIVKFPE NQYFFANLVDYYSSSNQNDKAMQFADDMLAKDPNNKLYLYVKAYLYHNMKDYEKAIEFYK KTLDIDPAYAEACSNLGLVYLLQAQEYADKAPADINDPNYATAQAEIKKFYEAAKPYYEK ARELKPDQKDLWLQGLYRVYYNLNMGPEFEEIEKMM
Sequence length 396 AA
Subcellular LocationPeriplasm
FunctionContains tetratricopeptide repeat (TPR) domains that mediate protein-protein interactions.
Protein Structure
PDB ID
Glycosylation Status
Glycosylation TypeO (Thr) linked
Experimentally Validated Glycosite(s) in Full Length ProteinTranslation of the protein seems to occur seven codons upstream of the start codon (Met) with a predicted signal peptide (by LipoP) for periplasmic translocation. Therefore, in full length protein sequence the glycosylated residue position should be T87, T178 and T231.
Experimentally Validated Glycosite(s ) in Mature ProteinT80, T171, T224
Glycosite(s) Annotated Protein Sequence>tr|Q5LCH1|Q5LCH1_BACFN Putative uncharacterized protein OS=Bacteroides fragilis (strain ATCC 25285 / NCTC 9343) GN=BF2494 PE=4 SV=1 MVLLMAVSFAFAQEKNVKEAKSIAGEVKPDFAKAEQLINEALTNPETKDNAATWDVAGYI QKRINEKEMENAYLRKPYDT*(80)LKVYNSVLNMYNYYVKCDELAQIPNEKGKIKNKYRSANSK TILAERPNLINGGIQYFNLNKNEDALKYFAAYVDAATLPMMEKENLLEKDT*(171)ILPQVAYYA TLAADRVGDKDAVMKYAQYALKDKENGQFAMQLLTDAYKAKGDT*(224)AKWVEKLQEGIVKFPE NQYFFANLVDYYSSSNQNDKAMQFADDMLAKDPNNKLYLYVKAYLYHNMKDYEKAIEFYK KTLDIDPAYAEACSNLGLVYLLQAQEYADKAPADINDPNYATAQAEIKKFYEAAKPYYEK ARELKPDQKDLWLQGLYRVYYNLNMGPEFEEIEKMM
Sequence Around Glycosites (21 AA)ENAYLRKPYDTLKVYNSVLNM
MEKENLLEKDTILPQVAYYAT
LTDAYKAKGDTAKWVEKLQEG
Glycosite Sequence Logoseqlogo
Glycosite Sequence Logo
Technique(s) used for Glycosylation DetectionMass shift detected on SDS-polyacrylamide gel, AAL (Aleuria aurantia lectin) reactivity, Pro-Q Emerald Glycostaining and reactivity towards anti-glycan antiserum
Technique(s) used for Glycosylated Residue(s) DetectionSite-directed mutagenesis
Protein Glycosylation- ImplicationProtein glycosylation is central to the physiology of B. fragilis and is necessary for the organism to competitively colonize the mammalian intestine. Deletion of the lfg (protein glycosylation machinery) region results in a substantial growth deficiency in vitro and a complete inability to compete with wild-type bacteria in the mouse intestine.
Glycan Information
Glycan AnnotationExogenous fucose
Technique(s) used for Glycan IdentificationLectin (AAL)binding
Protein Glycosylation linked (PGL) gene(s)
OST Gene NamewcfB
OST NCBI Gene ID3287270
OST GenBank Gene Sequence3287270
OST Protein NamePutative fucosyl transferase
OST UniProtKB/ SwissProt IDQ5LGI6
OST NCBI RefSeqYP_210702.1
OST EMBL-CDSCAH06753.1
OST UniProtKB Sequence>tr|Q5LGI6|Q5LGI6_BACFN Putative fucosyl transferase OS=Bacteroides fragilis (strain ATCC 25285 / NCTC 9343) GN=wcfB PE=4 SV=1 MLYVILRGRLGNNLFQIATAASLTQNFIFCTVNKDQERQVLLYKDSFFKNIKVMKGVPDG IPYYKEPFHEFSRIPYEEGKDLIIDGYFQSEKYFKRSVVLDLYRITDELRKKIWNICGNI LEKGETVSIHVRRGDYLKLPHALPFCGKSYYKNAIQYIGEDKIFIICSDDIDWCKKNFIG KRYYFIENTTPLLDLYIQSLCTHNIISNSSFSWWGAWLNENSNKIVIAPQMWFGISVKLG VSDLLPVSWVRLPNNYTLGRYCFALYKVVEDYLLNILRLIWKRKKNM
OST EC Number (BRENDA)
OST Genome ContextQ5LGI6
Characterized Accessory Gene(s)
PGL Additional LinksCAZy
Literatures
Reference(s)1) Fletcher, C.M., Coyne, M.J. and Comstock, L.E. (2011) Theoretical and experimental characterization of the scope of protein O-glycosylation in Bacteroides fragilis. J Biol Chem, 286, 3219-3226. [PubMed: 21115495]
2) Fletcher, C.M., Coyne, M.J., Villa, O.F., Chatzidaki-Livanis, M. and Comstock, L.E. (2009) A general O-glycosylation system important to the physiology of a major human intestinal symbiont. Cell, 137, 321-331. [PubMed: 19379697]
Additional CommentsGlycosylation sequon features: the sequon has an aspartate (D) preceding the glycosylated T or S which is followed by an amino acid with one or more methyl groups (alanine, isoleucine, or leucine; (D)(S/T)(A/I/L/V/M/T). Moreover, none of the 17 unglycosylated S and T residues examined in of BF2494 (excluding two in the signal peptide) have a preceding D, although seven are followed by A, I, or L and one by V. Non methylated amino acids were not tolerated at third position of sequon in BF2494. Il
Year of Identification2009
Year of Validation2009