| ProGlyProt ID | BC109 |
| Organism Information |
| Organism Name | Bacteroides fragilis (strain ATCC 25285 / NCTC 9343) |
| Domain | Bacteria |
| Classification | Family: Bacteroidaceae
Order: "Bacteroidales"
Class: "Bacteroidia"
Division or phylum: "Bacteroidetes" |
| Taxonomic ID (NCBI) | 272559 |
| Genome Sequence (s) |
| GeneBank | CR626927.1 |
| EMBL | CR626927 |
| Gene Information |
| Gene Name | BF2494 |
| NCBI Gene ID | 3287481 |
| GenBank Gene Sequence | 3287481 |
| Protein Information |
| Protein Name | BF2494 |
| UniProtKB/SwissProt ID | Q5LCH1 |
| NCBI RefSeq | YP_212118.1 |
| EMBL-CDS | CAH08194.1 |
| UniProtKB Sequence | >tr|Q5LCH1|Q5LCH1_BACFN Putative uncharacterized protein OS=Bacteroides fragilis (strain ATCC 25285 / NCTC 9343) GN=BF2494 PE=4 SV=1
MVLLMAVSFAFAQEKNVKEAKSIAGEVKPDFAKAEQLINEALTNPETKDNAATWDVAGYI
QKRINEKEMENAYLRKPYDTLKVYNSVLNMYNYYVKCDELAQIPNEKGKIKNKYRSANSK
TILAERPNLINGGIQYFNLNKNEDALKYFAAYVDAATLPMMEKENLLEKDTILPQVAYYA
TLAADRVGDKDAVMKYAQYALKDKENGQFAMQLLTDAYKAKGDTAKWVEKLQEGIVKFPE
NQYFFANLVDYYSSSNQNDKAMQFADDMLAKDPNNKLYLYVKAYLYHNMKDYEKAIEFYK
KTLDIDPAYAEACSNLGLVYLLQAQEYADKAPADINDPNYATAQAEIKKFYEAAKPYYEK
ARELKPDQKDLWLQGLYRVYYNLNMGPEFEEIEKMM
|
| Sequence length | 396 AA |
| Subcellular Location | Periplasm |
| Function | Contains tetratricopeptide repeat (TPR) domains that mediate protein-protein interactions.
|
| Protein Structure |
| PDB ID | |
| Glycosylation Status |
| Glycosylation Type | O (Thr) linked |
| Experimentally Validated Glycosite(s) in Full Length Protein | Translation of the protein seems to occur seven codons upstream of the start codon (Met) with a predicted signal peptide (by LipoP) for periplasmic translocation. Therefore, in full length protein sequence the glycosylated residue position should be T87, T178 and T231.
|
| Experimentally Validated Glycosite(s ) in Mature Protein | T80, T171, T224
|
| Glycosite(s) Annotated Protein Sequence | >tr|Q5LCH1|Q5LCH1_BACFN Putative uncharacterized protein OS=Bacteroides fragilis (strain ATCC 25285 / NCTC 9343) GN=BF2494 PE=4 SV=1
MVLLMAVSFAFAQEKNVKEAKSIAGEVKPDFAKAEQLINEALTNPETKDNAATWDVAGYI
QKRINEKEMENAYLRKPYDT*(80)LKVYNSVLNMYNYYVKCDELAQIPNEKGKIKNKYRSANSK
TILAERPNLINGGIQYFNLNKNEDALKYFAAYVDAATLPMMEKENLLEKDT*(171)ILPQVAYYA
TLAADRVGDKDAVMKYAQYALKDKENGQFAMQLLTDAYKAKGDT*(224)AKWVEKLQEGIVKFPE
NQYFFANLVDYYSSSNQNDKAMQFADDMLAKDPNNKLYLYVKAYLYHNMKDYEKAIEFYK
KTLDIDPAYAEACSNLGLVYLLQAQEYADKAPADINDPNYATAQAEIKKFYEAAKPYYEK
ARELKPDQKDLWLQGLYRVYYNLNMGPEFEEIEKMM
|
| Sequence Around Glycosites (21 AA) | ENAYLRKPYDTLKVYNSVLNM
MEKENLLEKDTILPQVAYYAT
LTDAYKAKGDTAKWVEKLQEG
|
| Glycosite Sequence Logo | seqlogo |
| Glycosite Sequence Logo |  |
| Technique(s) used for Glycosylation Detection | Mass shift detected on SDS-polyacrylamide gel, AAL (Aleuria aurantia lectin) reactivity, Pro-Q Emerald Glycostaining and reactivity towards anti-glycan antiserum |
| Technique(s) used for Glycosylated Residue(s) Detection | Site-directed mutagenesis |
| Protein Glycosylation- Implication | Protein glycosylation is central to the physiology of B. fragilis and is necessary for the organism to competitively colonize the mammalian intestine. Deletion of the lfg (protein glycosylation machinery) region results in a substantial growth deficiency in vitro and a complete inability to compete with wild-type bacteria in the mouse intestine. |
| Glycan Information |
| Glycan Annotation | Exogenous fucose |
| Technique(s) used for Glycan Identification | Lectin (AAL)binding |
| Protein Glycosylation linked (PGL) gene(s) |
| OST Gene Name | wcfB |
| OST NCBI Gene ID | 3287270 |
| OST GenBank Gene Sequence | 3287270 |
| OST Protein Name | Putative fucosyl transferase |
| OST UniProtKB/ SwissProt ID | Q5LGI6 |
| OST NCBI RefSeq | YP_210702.1 |
| OST EMBL-CDS | CAH06753.1 |
| OST UniProtKB Sequence | >tr|Q5LGI6|Q5LGI6_BACFN Putative fucosyl transferase OS=Bacteroides fragilis (strain ATCC 25285 / NCTC 9343) GN=wcfB PE=4 SV=1
MLYVILRGRLGNNLFQIATAASLTQNFIFCTVNKDQERQVLLYKDSFFKNIKVMKGVPDG
IPYYKEPFHEFSRIPYEEGKDLIIDGYFQSEKYFKRSVVLDLYRITDELRKKIWNICGNI
LEKGETVSIHVRRGDYLKLPHALPFCGKSYYKNAIQYIGEDKIFIICSDDIDWCKKNFIG
KRYYFIENTTPLLDLYIQSLCTHNIISNSSFSWWGAWLNENSNKIVIAPQMWFGISVKLG
VSDLLPVSWVRLPNNYTLGRYCFALYKVVEDYLLNILRLIWKRKKNM |
| OST EC Number (BRENDA) | |
| OST Genome Context | Q5LGI6 |
| Characterized Accessory Gene(s) | |
| PGL Additional Links | CAZy |
| Literatures |
| Reference(s) | 1) Fletcher, C.M., Coyne, M.J. and Comstock, L.E. (2011) Theoretical and experimental characterization of the scope of protein O-glycosylation in Bacteroides fragilis. J Biol Chem, 286, 3219-3226. [PubMed: 21115495]
2) Fletcher, C.M., Coyne, M.J., Villa, O.F., Chatzidaki-Livanis, M. and Comstock, L.E. (2009) A general O-glycosylation system important to the physiology of a major human intestinal symbiont. Cell, 137, 321-331. [PubMed: 19379697] |
| Additional Comments | Glycosylation sequon features: the sequon has an aspartate (D) preceding the glycosylated T or S which is followed by an amino acid with one or more methyl groups (alanine, isoleucine, or leucine; (D)(S/T)(A/I/L/V/M/T). Moreover, none of the 17 unglycosylated S and T residues examined in of BF2494 (excluding two in the signal peptide) have a preceding D, although seven are followed by A, I, or L and one by V. Non methylated amino acids were not tolerated at third position of sequon in BF2494. Il |
| Year of Identification | 2009 |
| Year of Validation | 2009 |
